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Cytiva, now with the life sciences business from Pall Corporation, is a global biotechnology leader dedicated to helping customers discover and commercialize the next generation of therapeutics. Cytiva brings dedicated technical expertise and a broad portfolio of tools, technologies, and services that enable the development, manufacture, and delivery of transformative medicines to patients.

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Chromatography

Chromatography Consumables

Chromatography Equipment

Chromatograph Columns

Reagents and Buffers

Chromatography Sample Preparation Devices

Chromatographs

Equipment Accessories

Liquid chromatography LC columns

Liquid Chromatograph (LC) Modules

Chromatography Software

Chromatography Media

Buffers for Protein Methods

Gas Chromatographs (GC)

Liquid Chromatographs (LC)

Liquid Chromatograph (LC) Fraction Collectors

$0~$10(0)
$10~$25(2)
$25~$50(6)
$50~$75(3)
$75~$100(8)
$100~$200(36)
$200~$300(59)
$300~$400(15)
$400~$500(25)
$500~$750(54)
$750~$1000(34)
$1000~$5000(131)
$5000~$10000(24)
$10000~$50000(12)
$50000~$100000(6)
$100000~$3000000(3)

Additives

Without: Calcium, magnesium, phenol red

Without: Phenol red

Without: Calcium, magnesium

Application

For purification of DNA from small molecules by gel filtration

Designed for use with a syringe, peristaltic pump, or chromatography system

Entry level chromatography solution for the preparative purification of proteins in lab scale.

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function

Flexible and intuitive chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram to gram levels of target product.

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Purification is done under physiological conditions and mild elution preserves the activity of the target protein

Routine UHPLC/HPLC analysis. Composite assays. Content uniformity testing. Protein precipitation. Solubility testing. Dissolution testing. Sample filtration. Use with any compound that requires protection from light, such as catecholamines or vitamins.

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

Tailored and reliable chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram to tens of gram levels of target product.

Affinity Chromatography, Gel Filtration/ Size Exclusion Chromatography, Ion Exchange Chromatography , Hydrophobic Interaction Chromatography and Reversed Phase Chromatography

Highly suitable for preparative purification of histidinetagged recombinant proteins

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

Application_4

Centrifugation

Chromatography system

Cell isolation

Chromatography system|Manual

Avoid Using

Chelating agents such as EDTA, EGTA , citrate

Oxidizing agents, citrate buffers

Not stable below pH 2 nor to strong oxidising agents

Bed Height

300 mm

100 mm

25 mm

600 mm

30 mm

Bed Height With One Adaptor

579 mm-609 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

0 mm-29 mm

85 mm-109 mm

129 mm-159 mm

185 mm-209 mm

825 mm-950 mm

225 mm-350 mm

525 mm-650 mm

Bed Volume

5 mL

120

~ 2.4 mL

1 mL

1 ml TALON® Superflow /column

212 mL

320

Bed Height With Two Adaptors

96 mm-156 mm

380 mm-650 mm

546 mm-606 mm

650 mm-950 mm

58 mm - 106 mm

80 mm-350 mm

0 mm-26 mm

158 mm-206 mm

0 to 200 mm

0-280 mm

680 mm-950 mm

85 mm-350 mm

Application_5

Chromatography system|Manual

Average sample loading capacity

50 mg

400 mg

Bed Support

Net

Frit

Filter

Bead type

Mag Sepharose Magnetic Beads

Binding Capacity/ml Chromatography Medium

Approx. 35 mg human lgG/mL at 2.4 min residence time

Approx. 40 mg histidine-tagged protein

At least 40 mg histidine-tagged protein/mL resin

Up to 20 mg histidine-tagged protein

~ 13 mg ovalbumin

At least 10 mg histidine-tagged protein

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

> 25 mg horse liver GST/mL resin

Bed Dimensions

10 x 100 mm

26 mm x 400 mm

6.4 x 100 mm

16 x 25 mm

26 x 600 mm

16 x 30 mm

3.2 x 300 mm

16 x 100 mm

16 x 600 mm

0.7 x 2.5 cm

1.6 x 2.5 cm

7 x 25 mm

Binding Capacity/Column

At least 10 mg histidine-tagged protein/ml sedimented medium.

Approx. 40 mg histidine-tagged protein/ml medium

up to 15mg histidine-tagged protein

125 mg human IgG/column

Autosampler Injection

No (optional)

Buffer Selection

2A + 2B inlets in one valve

Up to 14 inlets

Upgradeable to 14 inlets

2 buffer inlets

BioProcess resin

yes

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

Chemical Stability

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to acetone, ketones, chlorinated hydrocarbons, aliphatic esters, phenol, NaOH, >10% HCl, >5% acetic acid, or strong mineral acid.

Stable in all commonly used buffer systems

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea

Stable in commonly used aqueous buffers - 70% ethanol, 6 M guanidine hydrochloric, 8 M urea

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Commonly used aqueous buffers

Commonly used aqueous buffers,1 M sodium hydroxide6, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Conditions where the resin may be operated without significant change in function:
in¢ Trifluoroacetic acid, up to 0.2%
in¢ Isopropanol, up to 5%
in¢ Dithiothreitol (DTT), up to 5 mM
in¢ Acetonitrile, up to 70%
in¢ Formic acid, up to 1

Stable in all commonly used aqueous buffers

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules

For use with all aqueous solutions and organic solvents commonly employed in chromatography

Stable in aqueous buffers commonly used in Protein A Chromatography

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

For Ni²⁺-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Stable in commonly used aqueous buffers, 1 M NaOH

Certificate of Analysis

Yes

Chromatography technique

Hydrophobic Interaction Chromatography

Ion Exchange Chromatography

Antibody affinity chromatography

GST-tagged protein purification

Affinity coupling

Histidine-tagged protein purification

Antibody affinity chromatography.

Group specific affinity

Multimodal ion exchange chromatography

Column i.d.

5 mm

3.2

50 mm

6.4

26 mm

10 mm

16 mm

Cooling capacity

not applicable

Minimum: 4 Â°C Â± 2 Â°C. Maximum: ambient temp -3 Â°C. Measured as air temperature in sample compartment for maximum ambient temperature 25Â°C and maximum humidity 80%.

Minimum: 4 Â°C Â± 2 Â°C. Maximum: ambient temp -3 Â°C. Measured as air temperature in sample compartment for maximum ambient temperature 25 Â°C and maximum humidity 80%.

Density Max.

1.078 g/ml

1.135 g/ml|

1.2 g/ml

Depth

470 mm

280 mm

120 mm

Column volume

8 mL

1 mL

Connections

1/16" (eg Valco)

UNF 10-32 2B fingertights for capillary tubing o.d. 1/16"

Color

Colorless to slightly yellow

White

Color_20

Grey

Column Capacity

< 1 mg/mL

Conductivity monitoring

0.01 to 999.99 mS/cm

Dimensions_29

20 x 40 cm

36 mm

300 Ã— 360 Ã— 575 mm (W Ã— H Ã— D)

300 Ã— 360 Ã— 510 mm (W Ã— H Ã— D)

75 mm

Dimensions_33

75 mm

36 mm

20 x 40 cm

Dimensions

H x W x D: 660 x 860 x 710

Endotoxin Activity Max.

0.12 EU/mg

< 0.12 EU/ml

2 EU/ml

Flow Rate

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 mL/min

> 4 mL/min

< 20 mL/min |< 20 mL/min

> 20 ml/min|> 20 ml/min

1-4ml/min

1.6 mL/min

< 2.6 ml/min

1.2 - 2.3

2 ml/min - 10 ml/min

< 20 mL/min

< DarcyÂ´s Law

4 mL/min (1 mL), 5 mL/min (5ml)

< 15 ml/min

< 15 ml/min|< 15 ml/min

0.01 to 150 mL/min (normal range), 0.01 to 300 mL/min (column packing flow)

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

5 ml/min - 20 ml/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

< 100 ml/min

0.001 to 25 mL/min (normal range), 0.01 to 50 mL/min (column packing flow)

0.01-150 ml/min

0.8-3.9 ml/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

>10 ml/min

>60 ml/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

0.01 ml/min - 50 ml/min (AccuracyÂ± 2%)

0.01 ml/min-150 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.5-100 ml/min

0.8â€“3.9 mL/min

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

0.001 ml/min-25 ml/min

1 ml/min - 4 ml/min

< 1 mL/min

< 5 mL/min

0.5 - 5 ml/min

0.01 to 25 mL/min

0.5 ml/min - 2.5 ml/min

1 mL/min|1 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

Flow Velocity

< 450 cm/h

< 450 cm/h|< 450 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 600 cm/h

‰¤ 600 cm/h|‰¤ 600 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

100 cm/h - 300 cm/h 1

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

< 150 cm/h

<15 cm/h

Up to 40 cm/h

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

< 500 cm/h

30-150cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 500 cm/h|< 500 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

For Use With

AKTA lab protein purification system such as AKTA go, AKTA pure and AKTA avant

Connect a syringe to the top of a HiTrap columns.

Biacore 8K+, Biacore 8K, Biacore T200, Biacore S200 and Biacore 4000 SPR systems.

C 10 columns

The volumes are designed for Biacore 8K+, Biacore 8K but the kit can be used with all Biacore SPR systems

The new multi-functional V9-series valves are designed to be used together with AKTA avant

Tubing o.d. 3/16"

For Use With AKTA start

HiTrap and HiPrep columns (connecting column to AKTAdesign systems).

Tubing o.d. 1/16"

PD-10 Desalting Columns

AKTA avant and AKTA pure systems, and HiScreen or HiPrep columns of new design

For all Biacore SPR systems.

AKTAexplorer, AKTApurifier, AKTAbasic, AKTAFPLC

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

Filtrate Vessel Material

Polycarbonate (PC)

Flow Cell Path Length

2 mm (optional 0.5 mm and 10 mm)

2 mm (optional 10 mm)

2 mm (optional 10 mm and 0.5 mm)

2 mm (optional 5 mm)

Estimated Shelf Life from Manufacture Date

8 years

5 years

3 years

Exclusion Limit Peptides

4 x 10Â³

Dimensions_1

36 mm

75 mm

20 x 40 cm

Format_6

Bulk resin

Kits

Prepacked column

Prepacked columns

For use with1

AKTA avant and AKTA pure systems

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

Flow Channel id

0.8 mm

For use with_31

AKTA lab protein purification system such as AKTA go, AKTA pure and AKTA avant

AKTA go protein purification system

Format

Liquid

Flow Direction

Supported bidirectionally. Certain applications may require orientation, i.e., vents. Reverse flow only for low pressure applications.

Flow should follow arrows

Guidance

For one column

FR-902 is pre-set to 0.2 MPa and is used when using low pressure columns and/or when the pH electrode is mounted in the pH flow cell.

The kit contains:
1 x 25 Âµl sample loop i.d. 0.5 mm
1 x 50 Âµl sample loop i.d. 0.5 mm
1 x 100 Âµl sample loop i.d. 0.5 mm
1 x 200 Âµl sample loop i.d. 0.8 mm
1 x 500 Âµl sample loop i.d. 0.8 mm
Each sample loop consists of tubing

The kit contains 1 x Fill port 6 x Needle 1 x Syringe holder *The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100 % Ethyl acetate, 100 % Hexane and 100 % Tetrahydrofuran.

To connect the air sensor use: Tubing i.d. 1.6 mm o.d. 1/8" Tubing connector 1/8" with Ferrule 1/8" Adapter for air sensor

Lamp and aperture are supplied with Hg lamp and housing

The tubing is fitted with 2 x Nut UNF 5/16" male 2 x Ferrule *The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100 % Ethyl acetate, 100 % Hexane and 100 % Tetrahydrofuran.

Tubing and connectors have to be ordered separately.

The kit contains 1 x Inlet check valve 1 x Outlet check valve *The wetted parts in the check valv kit are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100 % Ethyl acetate, 100 % Hexane and 100 %

To connect the air sensor use: Tubing i.d. 0.5 mm o.d. 1/16" Fingertight connector 1/16" Adapter for air sensor

For AKTAprime

*The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100 % Ethyl acetate, 100 % Hexane and 100 % Tetrahydrofuran.

When ordered separately the connectors are black.

Fitting 1/16" in PEEK

Max flow rate: 100 ml/min Max pressure: 25 MPa The kit contains 1 x Channel plate 1 x Distribution plate 1 x Allen key *The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100 %

The kit contains 3 x distributor block, 3 x channel plate, 1 x Allen key 3 mm, Instructions.

Use: Connection of syringe to top of column When ordered separately the connectors are black.

Water-resistant phosphorimaging plate, 20x40 cm, multipurpose

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Height

210 mm

482 mm

135 mm

660 mm

320 mm

629 mm

Filter Media

PES filter

Group To be Coupled

Primary amines

-NHâ‚‚

Injection valve

1/16â€ PEEK valve 6 port 2 position with 0.25 mm i.d. bore

1/16â€ PEEK Valcoâ„¢ valve, 6 port 2 position, with 0.75 mm i.d. bore

Injections per vial/well

Max. 9

Inlet Connector

Stepped barb for 10 to 12 mm (3/8 to 1/2") tubing

Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing

Inlet id

0.75 mm (0.029 in)

1 mm (0.04 in)

2 mm (0.08 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

Ligand

Alkaline-stabilized protein A-derived (E.coli).

Octylamine

Diethylaminoethyl

Carboxymethyl

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Calmodulin

Strep-Tactin XT

Sulphopropyl

Alkali-stabilized Protein A-derived (E. coli)

Porcine heparin

Recombinant protein A (E.coli)

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Native Protein A

TALONÂ® Superflow Cobalt-IMAC

Cibacron Blue

Human IgG

Quaternary amine

Ligand Density

~ 5 mg/mL resin

3 mg Protein A/ml medium

6 mg rProtein A/ml medium

3 mg GammaBind G, type 3/ml medium

Approx. 13 Î¼mol/ml

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

Matrix

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Highly cross-linked agarose, spherical.

agarose, 6%, spherical

2% agarose

Cross-linked dextran

Composite of cross-linked agarose

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Composite of cross-linked agarose and dextran

Highly cross-linked 6% agarose

Cross-linked agarose, 4%

Rigid, cross-linked agarose, spherical

cross-linked, 6% agarose particles

Highly cross-linked agarose

4% Agarose

4% cross-linked agarose

cross-linked agarose, 4%, spherical

Matrix

Highly crossed-linked agarose, 6%

6% Cross-Linked Agarose

Highly Cross-Linked Agarose with Dextran surface extender

Highly cross-linked 4% agarose

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Hold-up Volume of Membrane

<5 Î¼l

<10 Î¼l

<20 Î¼l

Housing

Polypropylene (PP)

Multi-Step Purification

Yes, (optional)

Multiple Sample Injection

Yes, up to 7 samples

Upgradeable to 5 samples

Multiple Wavelength Detection

Up to 3 wavelengths

Yes, up to 3 wavelengths

Fixed wavelength

Operating Flow Rate, Max

< 4 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.35 mL/min water at low temperature;
0.17 mL/min 20% ethanol or 10% glycerol at low temperature

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

1.0 mL/min

2.6 mL/min

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

Operating Pressure Max

5 MPa

10 bar (1 MPa)

10 MPa (1450 psi)

20 MPa (2900 psi)

4.1 bar (60 psi)

5 Mpa (725 psi)

250 bar (25 MPa) (3626 psi)

5 MPa (750 psi)

Order code

SH30031.01

SH30031.02

SH30268.01

SH30588.01

SH30031.FS

SH30237.01

SH30588.02

Order Information

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

Includes six default cassettes for fractionation: four cassettes for 50 mL tubes and two cassettes for 15 mL tubes. Also comes with three different mixers ( 1.4, 5 and 15 mL) and a complete set of inlet and outlet tubing.

Percoll PLUS is for research use only.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

For research use only.

Not for use with ultrasalt-sensitive automated DNA Sequencers (e.g. ALFexpress II and ABI 377 model.)

HiLoad columns are prepacked XK columns

Allows safe, reliable storage and transport of self-packed Tricorn columns.

Includes two default cassettes for fractionation. Up to six cassettes can be placed in the fraction collector for full capacity. Note, AKTA avant is not for use with fraction collector Frac-950. For ordering information, please contact your local GE Healt

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.

HiLoad columns are prepacked XK columns.

Not suitable for use with AKTAprime plus system.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

Columns included in HiTrap HIC Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow. PD- 10 Buffer reservoir has to be ordered separately For smaller s

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Pack size

2 x 1 ml 10% slurry

50 mL

1.7 mL

1/pk

100 mL

125 mL

25 g

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 Ã— 100 ÂµL

50 columns

6 mL

7 Ã— 1 mL

1 Ã— 120 mL

1 piece

20 mL

250 preps

300 mL

1 L

10 mL

200 mL

25 mL

4 x 50 mL

4 x 53 mL

500 g

6 x 500 mL

1 Ã— 1 mL

4.7 mL

5 Ã— 5 mL

50 preps

6 x 100 mL

75 mL

1 kit

1.5 g

10 Ã— 2 mL

5 Ã— 1 mL

6 x 1 L

1 Ã— 320 mL

1 Ã— 4.7 mL

10 g

2 x 1 mL

100 g

20 columns

30 columns

5 mL

1000 mL

15 g

250 columns

500 mL

53 mL

Partical Size Dry

18 Âµm-111 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

50 Âµm-150 Âµm

>100 Âµm

>20 Âµm

Particle size, d_50V

~90 Âµm

34 Âµm

~100 Î¼m

~34 Âµm

~34 Î¼m

~85 Âµm

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~ 8.6 Âµm

~50 Âµm

~75 Âµm

~90 Î¼m

pH Stability Short Term

1â€“14

pH stability, operational

2 to 12Â

2â€“10

pH 6 to 10

2â€“12

3â€“10

2 to 12

3â€“12

3â€“9

3 to 12

3â€“11

3â€“13

2â€“13

4â€“12

2â€“9

4â€“13

4â€“9

Physical State

Liquid

liquidSuspension

solid

Power Consumption

800 VA

Rated max. 300 VA; Max. with all options 150 W; Typical 100 W; Power-save < 20 W

300 VA

1.1 VA

8 VA

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

Includes

Installation instructions, PEEK tubing

Installation instructions and port positioning label

10 x Net ring 10 x Support screen

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Connectors for high- and low-pressure chromatography equipment and instructions.

Inlet Valve V9H-IA
FEP Tubing ID 2.9 mm
Connector 5/16" male for 3/16" o.d. tubing
Blue ferrule

Loop valve kit V9H-L
PEEK tubing i.d. 0.75 mm/o.d. 1/16''
PEEK tubing i.d. 1.0 mm/o.d. 1/16''
Fingertight connectors 1/16''

Loop valve kit V9-L
PEEK tubing i.d. 0.5 mm/o.d. 1/16''
Fingertight connectors 1/16''

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

Fill port, needle and syringe holder.

Inlet Valve V9H-IB
FEP Tubing ID 2.9 mm
Connector 5/16" male for 3/16" o.d. tubing
Blue ferrule

One fill port

UniNet cable.

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Column valve V9H-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 1.0 mm/o.d. 1/16''

No keyboard included â€“ please order separately. Country specific keyboards available.

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Instructions supplied with the concentractors

Outlet Valve V9H-O
FEP Tubing ID1.6mm
Connector 5/16" male for 1/8" o.d. tubing
Yellow ferrule

pH Valve V9H-pH
Tubing kit for V9H-pH
Dummy pH electrode

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Ion Exchanger Type

Weak and strong anion

Weak cation exchanger

Weak anion exchanger

Multimodal weak cation exchanger

Strong cation exchanger

Strong anion exchanger

Weak anion

Ionic Capacity

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.18-0.25 mmol H⁺/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.29-0.35 mmol Cl^-/ml medium

0.13-0.17 mmol/ml

0.15-0.20 mmol H⁺/mlÂ

40-85 Âµmol/ml (Cl^-)

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

Recommended Flow Rate

5 ml/min

1 ml/min

Safety certifications1

CE, cMETus, EU ROHS, China ROHS

Sample Volume

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

< 100 Âµl

<= 15 ml

<= 5 ml

100 to 300 Î¼l

Up to 8.5 mL

<= 13 ml

4 to 50 ÂµL

600 Âµl

2.5 ml

400 to 1000 Î¼l

0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)

25 to 500 ÂµL

< 150 Âµl

100-180 Î¼l

<= 1.5 ml

< 0.5 mL

< 5 mL

Kit Contents

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Aprotinin (10 mg): 6500 M_r
Ribonuclease A (50 mg): 13 700 M_r
Carbonic anhydrase (15 mg): 29 000 M_r
Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Blue Dextran 2000

1 sensor chip for maintenance procedures.

BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL

RNAspin Lysis Solution (Red sticker): 25 ml, RNAspin Wash Buffer I (Yellow sticker): 15 ml, RNAspin Wash Buffer II (Grey sticker): 12.5 ml Add 50 ml Ethanol; RNAspin Desalting Buffer (Blue sticker); 25 ml, RNAspin Desalting Reaction Buffer (Green sticker)

RNAspin Lysis Solution (Red sticker): 125 ml, RNAspin Wash Buffer I (Yellow sticker): 80 ml, RNAspin Wash Buffer II (Grey sticker): 3 Ã— 25 ml Add 100 ml Ethanol to each bottle; RNAspin Desalting Buffer (Blue sticker); 125 ml, RNAspin Desalting Reaction B

Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Aldolase (50 mg): 158 000 M_r
Ferritin (15 mg): 440 000 M_r
Thyroglobulin (50 mg): 669 000 M_r
Blue Dextran 2000 (50 mg

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand Concentration

~45 Âµmol phenyl/mL resin

~25 Âµmol phenyl/mL resin

~4mg heparin/mL resin

4.5-8 mg gelatin/ml drained resin

Injection volume1

Programmable from 0 to 5000 ÂµL 1 ÂµL increments. Maximum injection volume depends on installed sample loop and injection mode

Programmable from 0 to 19 999 ÂµL, 1 ÂµL increments. Injection volume depends on sample loop.

Length

3 m

2 m

Ligand_7

Protein L

2-mercaptopyridine

Protein A

Protein A, alkali stabilized

Protein A, alkali stablilized

Protein G

Butyl, octyl, phenyl

Material

Distributor block: Polyetheretherketone (PEEK)

Perfluorrubber (PFR) Polyetheretherketone (PEEK) Stainless steel Titanium (Ti) Polytetrafluoroethene (PTFE)

Acetal plastic (POM)

Ethylenetetra fluorethylene (ETFE)

Perfluoroethylene-propylene copolymer (FEP)

Polyetheretherketone (PEEK)

Acetal plastic (POM) Fluor carbon rubber (FPM) Polypropylene (PP)

FFKM, Fully fluorinated perfluoroelastomer PEEK, Polyetheretherketone Stainless steel

Polyoxymethylene (POM-C)

Ethylene propylene diene (EPDM) Polyetheretherketone (PEEK)

Injection fillingport:Ethylenetetra fluoroethylene (ETFE) Titanium (Ti)

Polycarbonate (PC)

Polypropylene (PP)

Borosilicate glass Ethylene propylene diene (EPDM) Polyetheretherketone (PEEK) Polytetrafluoroethylene (PTFE)

Ethylenetetra fluoroethylene (ETFE), Titanium (Ti)

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

Ethylenetetra fluoroethylene (ETFE)

Etylenetetra fluoroethylene(ETFE) Polypropylene (PP)

Ligand Coupling Method

Reductive Amination

Ether linkages and hydrophilic spacer arm

Epoxy

Maleimide activation

Dextran surface extenders

Cyanogen bromide activation

Triazine

Ether linkages

Epoxy activation

Ligand

Recombinant GammaBind G type 3 lacking albumin-binding region

Inner Diameter

1 mm (0.04 in)

0.3 mm (0.011 in)

0.75 mm (0.029 in)

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Sterilization

May be autoclaved at 121Â°C for 20 minutes. Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed after autoclavin

0.1 Âµm sterile filtered

Storage

+4Â°C to +8Â°C

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

20 to 25Â°C, Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative

20 to 25Â°C, TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.

4 to 30Â°C

4 to 30Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

4 to 30Â°C, 0,05% Kathon CG/ICP Biocide

4 to 30Â°C, 0.15% Kathon CG

4 to 30Â°C, 20% Ethanol (Q, DEAE), 20% Ethanol, 0.2 M Sodium Acetate (S)

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.05 M Sodium Acetate containing 20% Ethanol

4â€“30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

4 to 30ËšC

4Â°C to 8Â°C

4 to 30Â°C, in 20% Ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP)

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% ethanol

2 to 8Â°C, 100% Isopropanol

4 to 30Â°C, 20% Ethanol or 70% Acetonitrile

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

2Â° to 8Â° C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

Number of Ports

Particle Size

24-44 Âµm

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

60 Î¼m-160 Î¼m

60 Î¼m-200 Î¼m

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

60 Âµm-165 Âµm

60 Âµm

Particle size distribution, wet

15â€“88 Âµm

90â€“280 Âµm

55â€“165 Âµm

87â€“510 Âµm

100â€“300 Âµm

20â€“80 Âµm

Operating Flow Rate, Recommended

0.04 mL/min

0.075 mL/min

0.80 mL/min

0.30 mL/min

0.45 mL/min

0.50 mL/min

0.75 mL/min

1.3 mL/min

0.5 mL/min

pH Range

14-Jan

8.5â€“9.5

5.5â€“7.5

8.9â€“9.9

pH stability, CIP

3â€“13

2â€“14Co²⁺-stripped medium

1â€“14

2â€“14

2â€“12

2â€“10

3â€“14

2â€“10.5

3â€“10

4â€“13

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

2â€“13

2â€“13.7

3â€“12

Operating Temperature

<40Â°C

4Â°C-60Â°C

4Â°C-35Â°C

4Â°C-40Â°C

Outer Diameter

10-21 mm

25 mm (0.98 in)

12 or 18 mm

1.58 mm (0.06 in)

1.58 mm (1/16 in)

10 - 50 mm

Pack Size

7.0 to 7.4

7.2 - 7.5

pH Stability Cleaning-in-Place (CIP)

0 to 14

1 to 14

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Pore Size

0.2 Âµm

1 Âµm

0.45

Osmolality

< 25 mOsm/kg

Porosity

10 Î¼m

20 Î¼m

10 or 80 Âµm

7 Î¼m

Pressure/Flow Specification

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

pH ligand fully charged

Above 6

Below 9

< 9

Entire pH range

Particle Size in Methanol Min

pH Stability Long Term

43843

pH Stability Working Range

3â€“12

Power requirements

95 to 240 Volt AC Â± 10%; 50 to 60 Hz; 200 VA

Pressure Limit

2 MPa

20 bar (2 MPa, 290 psi)

Protection Class

IP 20

IP 21, wet side IP 22

IP 21

Osmolality

< 30 mOsm/kg

276-298 mOsm/kg

322-344 mOsm/kg

288-310 mOsm/kg

Particle size distribution, dry

20 â€“ 80 Âµm

50 â€“ 150 Âµm

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Recommended maximum operational flow velocity

300 cm/h

Relative humidity1

20% to 80% RH

Resin Type

Capto

Sepharose Fast Flow

MabSelect

Sepharose CL-4B

Superdex 200 Increase

Sepharose High Performance

Tubing id

0.5 mm

Scale

Lab

Lab|Process development|Process

Lab|Process

Lab|Process development

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

UV Wavelength

190 to 700 nm

190-700 nm

280 nm

Outlet id

1.55 mm (0.06 in)

0.75 mm (0.029 in)

1.0 mm (0.04 in)

Vent

On inlet

Volume_28

400 Âµl - 2 ml

2-6 ml

5-20 ml

100-500 Âµl

Weight

Up to 53

Wetted Materials

Polyetheretherketone (PEEK), polytetrafluoroethene (PTFE)

Polyetheretherketone (PEEK)/Fluoroethenepropene (FEP)

etheretherketone (PEEK)/Ethylenetetrafluoroethylene (ETFE)

Ethylenetetra fluoroethylene (ETFE)

Fully fluorinated perfluoroelastomer (FFKM) Polyetheretherketone (PEEK)

Injection fillingport:Ethylenetetra fluoroethylene (ETFE) Titanium (Ti)

Polyetheretherketone (PEEK)

Fluoroethenepropene (FEP)/Polyetheretherketone (PEEK)/PolyChloroTriFluoroEthylene(PCTFE)

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

Polypropylene (PP)

therketone (PEEK)/Polytetrafluoroethylene (PTFE)

Polypropylene (PP) Fluor rubber (FPM) Acetal Plastic (POM)

Polyetheretherketone (PEEK)/ Fluorinated Ethylene Propylene (FEP)/ PolyChloroTriFluoroEthylene (PCTFE)

Polyetheretherketone (PEEK)/ Polytetrafluoroethene (PTFE)/ Ethylene TetraFluoroEthylene (EFTE)/ Fully Fluorinated Propylene Monomer (FFPM/FFKM)

Polyetheretherketone (PEEK)/Ethylenetetrafluoroethylene (ETFE)

Polyetheretherketone (PEEK)/Fluorinated Ethylene Propylene (FEP)

Polyetheretherketone (PEEK)/Fluoroethenepropene (FEP)/Ethylenetetrafluoroethylene (ETFE)

Polyetheretherketone (PEEK)/Polytetrafluoroethene (PTFE)

Wetted materials1

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

Polyetheretherketone (PEEK)

Wetting Characteristics

Hydrophilic

Safety Certifications

2006/42/EC (MD), 2004/108/EC (EMC), 1999/5/EC (R&TTE)

Options

Cassette options 50 ml tubes: 6 tubes/cassette 15 ml tubes: 15 tubes/cassette 8 ml tubes: 24 tubes/cassette 3 ml tubes: 40 tubes/cassette One deep well plate (24, 48 or 96 wells) per cassette Rack options 50 ml tubes: 55 tubes per rack 250

Outlet Connector

Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing

Stepped barb for 10 to 12 mm (3/8 to 1/2") tubing

Sampler capacity

Vial rack with 108 positions for (1.5 mL). Microplates according to SBS standards. 96-well (deep-well and standard) as well as 384-well

Two Vial racks of 12 vials each for 10 mL vials. Maximum vial height 47 mm. Minimum vial height 32 mm.

Sealing

Heat-fused

Shelf Life

12 months

24 Months

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

pH Monitoring

0â€“14

0 to 14 (optional)

0 to 14

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Operating temperature1

4 Â°C to 40 Â°C; indoor use only

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Sample Volume Min

1 ml

Power Requirement

32 VDC from Pump P-901

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Storage conditions1

-25 Â°C to 60 Â°C

Swelling factor

~1.7

Stability

Stable

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Storage Conditions

Store at 2Â°C to 8Â°C

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

4â€“30Â°C

20% ethanol at 4Â°C to 30Â°C

4 - 30Â°C

At room temperature. Precipitation can occur at low temperature.

Store at room temperature.

Sample Volume Max

150 ml

Relative Humidity

20%-95% (non condensing)

20% to 95% (noncondensing)

20%-95%

Pump Type

Piston pump, metering type

Sample loop

10 mL

Default 100 ÂµL

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Theoretical Plates

5000 m-1

> 13000 m-1

> 48 000 m^-1

> 43 000 m^-1

> 38 000 m^-1

> 42 000 m^-1

Support System

Polypropylene (PP)

Syringe volume

500 ÂµL standard; 2500 ÂµL optional

2500 ÂµL

Viscosity

0.35 to 10 cP (system pump), 0.7 to 10 cP (sample pump)

0.35 to 10.0 cP (system pump)

0.35 to 5.0 cP (system pump), 0.7 to 10.0 cP (sample pump)

0.35 to 5 cP (system pump), 0.7 to 10 cP (sample pump)

Width

335

860

215

535

Viscosity Max

0.7 to 10

Total Time of Preparation

< 30 min/6 preps

Wetted Part

yes

System

Sepax C-Pro|Sefia|Sepax 2

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Voltage

100 to 120/220 to 240 VAC

100-120/220-240 VAC

Voltage

100 to 240 VAC

Volume

2-6 ml

100-500 Âµl

400 Âµl - 2 ml

5-20 ml

Weight_30

19 kg

21 kg

Water Flow Rate at 10 bar (145 psi)

Type

Fingertight 1/16"

Total binding capacity

~50 mg hIgG/mL resin

Valve Type

Motor valve

Well Volume

8.5

Wetted part1

PEEK, PTFE, Tefzel, Vespel, PCTFE, glass. Needle coated with silica coating on SS316 (inside and outside)

PEEK, PTFE, Tefzelâ„¢, Vespelâ„¢, PCTFE, glass. Needle coated with silica on stainless steel SS316 (inside and outside)

Intrinsic Viscosity

~ 17

Min Pressure for Seal Movement

1 bar [0.1 MPa] (14 psi)

Particle Size in Methanol Max

163

Stokes Radius

~ 10

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