Ficoll PM 400 density gradient media, 500 kg
For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
Concentrations of up to 5NA% (w/v) covering densities of up to 1.2 g/ml can be achieved.
Better osmotic properties than sucrose.
Preserves functional and morphological integrity.
Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
Does not penetrate biological membranes.
Serves as the raw material for preparation of FicollPaque gradients.Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.Ficoll PM400 is a synthetic neutral, highlybranched hydrophilic polymer of sucrose with an average molecular weight of 400 000. It has long been used to form density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells, as a stabilizing agent, and as a preparation medium for isolating mononuclear cells.