HisTrap HP His tag protein purification columns, 5 x 5 ml
High binding capacity, at least 4NA mg/ml chromatography medium.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Negligible Ni2+ leakage
Simple manual operation with a syringe, pump, or chromatography system such as ÄKTA design
HisTrap HP 1 ml and 5 ml columns are designed for simple, onestep purification of histidinetagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.
HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)1NA. The shorter (histidine)4 will bind more weakly and the longer (histidine)1NA will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)1NA binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be copurified with the tagged target protein.
The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions. For convenient scaling up of histidinetagged protein purification, use 2NA ml HisPrep FF 16/1NA columns. Ni Sepharose 6 Fast Flow, the medium prepacked in HisTrap FF and HisPrep FF 16/1NA columns, allows high flow rates, which facilitates scaleup of histidinetagged protein purification.