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Cytiva, now with the life sciences business from Pall Corporation, is a global biotechnology leader dedicated to helping customers discover and commercialize the next generation of therapeutics. Cytiva brings dedicated technical expertise and a broad portfolio of tools, technologies, and services that enable the development, manufacture, and delivery of transformative medicines to patients.

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Silylation Reagents

Sponges

Tubes for Blood Collection

$0~$10(0)
$10~$25(1)
$25~$50(194)
$50~$75(41)
$75~$100(19)
$100~$200(73)
$200~$300(54)
$300~$400(21)
$400~$500(32)
$500~$750(40)
$750~$1000(29)
$1000~$5000(72)
$5000~$10000(2)
$10000~$50000(2)
$50000~$100000(0)
$100000~$3000000(0)

Absorbance

0.02 AU max. at 330nm

Absorbance

Pass Test

Additives

Without: Calcium, magnesium

Without: Phenol red

Without: Calcium, magnesium, phenol red

Aluminum Al

20 ppb max.

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Amount per Unit

4X4L

Analysis Time

20 to 200 min.

Application_5

Chromatography system|Manual

Application

For the removal and/or purification of serine proteases e.g. thrombin

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Application_4

Cell isolation

Chromatography system|Manual

Assay Percent Range

>=99 %

99.8% min by GC

Applications

thin layer chromatography (TLC): suitable

HPTLC: suitable

Bed Height

100 mm

25 mm

30 mm

600 mm

Autoignition Temp

800 °F

Bed Dimensions

16 x 25 mm

16 x 100 mm

16 x 30 mm

7 x 25 mm

Bed Volume

5 mL

1 mL

320

Assay

=99%

Assay Percent Notes

99% min

Binding Capacity/Column

At least 10 mg histidine-tagged protein/ml sedimented medium.

Approx. 40 mg histidine-tagged protein/ml medium

Capacity

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

0.8 meq/mL by wetted bed volume

3.8 meq/mL by wetted bed volume

1.7 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

4.7 meq/g by dry weight

Bead type

Mag Sepharose Magnetic Beads

Boiling Point

121-122 C (lit.)

130-132 C (lit.)

2230 C

189°C

45-50 C/14 mmHg (lit.)

Binding Capacity/ml Chromatography Medium

At least 10 mg histidine-tagged protein

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

BioProcess resin

yes

Buffer

Running Buffers

Calcium Ca

50 ppb max.

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

CAS Registry Number 1

631-61-8

540-69-2

91000-53-2

67-68-5

7631-86-9

9004-34-6

CAS_NO

112926-00-8

1343-88-0

161308-36-7

17287-03-5

2042-27-5

373-57-9

7631-86-9

9003-70-7

9041-35-4

9050-97-9

104219-63-8

11128-95-3

126850-14-4

110-85-0

1344-28-1

15461-27-5

24589-78-4

39389-20-3

1066-33-7

112945-52-5

60177-39-1

62610-50-8

65099-79-8

685-27-8

69011-15-0

69011-19-4

63181-98-6

65546-95-4

69011-20-7

25561-30-2

39339-85-0

75-09-2

7647-01-0

77377-52-7

9004-34-6

9004-54-0

9052-45-3

ChEBI

CHEBI:28262

CHEBI:62947

CHEBI:63050

CHEBI:30563

Chemical Name or Material

Ammonium Acetate

Silica Gel 60

Ammonium formate

Cellulose

Dimethylsulfoxide

TAPS buffer soln.

Silica Gel

Chemical Stability

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in all commonly used buffer systems

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Commonly used aqueous buffers

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Stable in commonly used aqueous buffers, 1 M NaOH

Certificate of Analysis

Yes

Chemical Substance ID

24852120

24852833

24854190

24857346

24857353

24864382

24896572

329750793

329752001

329759188

24852304

24853619

24854187

24854645

24857347

24857358

24848188

24852837

24854191

24854823

24857354

329817869

24854194

329759187

329763462

329759181

329763461

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chem Alert Storage Symbol

Gray

Chem AlertStorageSymbol

Gray

Chromatography technique

Hydrophobic Interaction Chromatography

Antibody affinity chromatography

Group specific affinity

GST-tagged protein purification

Histidine-tagged protein purification

Ion Exchange Chromatography

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Concentration

20 X

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Copper Cu

10 ppb max.

COLOR

Blue

White

Color

colorless

Color

Colorless to Slightly Yellow

Column i.d.

Composition

Tris Base, Sodium Chloride

Density

1.100g/cmÂ³

Density Max.

1.2 g/ml

Decomposition Information

>190°C

EC Number

231-545-4

215-609-9

215-681-1

238-878-4

Empirical Formula

MgO3Si

Endotoxin Activity Max.

0.12 EU/mg

Flash Point

87°C (188.6°F)

Flow Rate

< 20 ml/min|< 20 ml/min

< 4 mL/min

< 4 ml/min|< 4 ml/min

< 20 mL/min

< DarcyÂ´s Law

>10 ml/min

Format

matrix material

Estimated Shelf Life from Manufacture Date

5 years

Exclusion Limit Peptides

4 x 10Â³

Format_6

Prepacked columns

Bulk resin

Prepacked column

Formula

(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃

MgO₃Si

O2Si

Service & Support

Feature

No Flourescent Indicator

Fluorescent Indicator

Flow Velocity

< 150 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

<1800 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

Formula Weight

100.39

60.08

78.13

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Form

fine crystals

neat

fine powder

powder

powder or crystals

Form

Liquid

Excitation Wavelength

254 nm

For Use With Application

In-Process Control in Drug Synthesis, Identity and Stability Testing of Drugs, Quality Control of Pharmaceuticals, Food and Environmental Samples, Residue Analysis in Food and Environmental Samples

Format

Liquid

Formula Weight

77.08

Grade

for column chromatography

High-Purity Grade

High-purity Grade (9385)

high-purity grade (Davisil Grade 643)

high-purity grade (Davisil Grade 923)

HPLC

analytical standard

certified reference material

for GC derivatization

high-purity grade (Davisil Grade 633)

High-purity Grade (Davisil Grade 635)

high-purity grade (Davisil Grade 636)

Reagent Grade

Standard

Grade

Technical Grade

High-purity Grade (Davisil Grade 646)

Technical Grade 40

High-purity Grade (7754)

Grade 1 Chr

High-purity Grade (Davisil Grade 12)

Nuclear Grade

Health Hazard 2

WARNING!

CAUTION!

GHS H Statement
H335

H-Codes

H225-H314

H226 - H315 - H319 - H335

H319

H373

H226-H314

H372

Height English

0.0011 in.

Health Hazard 1

H500

H373: May cause damage to organs through prolonged or repeated exposure.

Identification

Pass Test

Ignition Residue

0.01% max.

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Emergency Overview
May cause skin, eye, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if s

GHS P Statement
P261-P271-P304+P340-P312-P405-P403+P233-P501a

Height Metric

30 µm

InChIKey

USFZMSVCRYTOJT-UHFFFAOYSA-N

FEGYIWVHCSRXCG-UHFFFAOYSA-M

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

VZTDIZULWFCMLS-UHFFFAOYSA-N

VYPSYNLAJGMNEJ-UHFFFAOYSA-N

Includes

18 Pc.

Includes

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Connectors for high- and low-pressure chromatography equipment and instructions.

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Insoluble Matter

0.005% max.

Intrinsic Viscosity

~ 17

IOD-Codes

F,T

F,C

Ion Exchanger Type

Strong anion exchanger

Weak anion

Multimodal weak cation exchanger

Strong cation exchanger

Weak anion exchanger

Weak and strong anion

Weak cation exchanger

Ionic Capacity

0.07-0.09 mmol H⁺/ml medium

0.15-0.20 mmol H⁺/mlÂ

0.18-0.25 mmol H⁺/ml medium

0.29-0.35 mmol Cl^-/ml medium

0.13-0.17 mmol/ml

0.18-0.24 mmol Cl^-/ml medium

0.09-0.13 mmol H⁺/ml medium

0.11â€“0.16 mmol (Cl^-)/mL resin

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

3-4 mmol/g

IUPAC Name

azanium;formate

sodium;3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonate

azanium;acetate

dioxosilane

methylsulfinylmethane

Length Metric

100 m

7.5 cm

20 cm

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand

Alkaline-stabilized protein A-derived (E.coli).

Bovine gelatin

Cibacron Blue

Glutathione and 10-carbon linker arm

Human IgG

Phenyl

Quaternary amine

Carboxymethyl

Porcine heparin

Sulphopropyl

Calmodulin

Native Protein A

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Alkali-stabilized Protein A-derived (E. coli)

Diethylaminoethyl

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Ligand Coupling Method

Cyanogen bromide activation

Epoxy activation

Triazine

Epoxy

Maleimide activation

Dextran surface extenders

Ether linkages

Ether linkages and hydrophilic spacer arm

Reductive Amination

Ligand Density

~ 5 mg/mL resin

4.5-8 mg gelatin/ml drained resin

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

3 mg GammaBind G, type 3/ml medium

3 mg Protein A/ml medium

Approx. 13 Î¼mol/ml

Ligand_7

Butyl, octyl, phenyl

Protein L

Protein A

Protein A, alkali stabilized

Protein G

Linear Formula

(CH3)3S(OH)

C9H18F3NOSi

SiO2

(C10H12 · C10H10 · C8H8 · C3H9N)X

CF3CON(CH3)COCF3

CF3CON(CH3)Si(CH3)3

HCO2NH4

MgO3Si

(C10H12 · C10H10 · C8H8)x

(CH₃)₂SO

CF3C[=NSi(CH3)3]OSi(CH3)3

CH3COONH4

HCl

Material

C 18 -silica gel 60 matrix

Glass Support

Polypropylene

Silica Gel 60 F254

silica gel matrix

Florisil

PEEK

PEI-cellulose, matrix

Plastic/Stainless Steel

Alumina

aluminum support

Cellulose

N/A

Silica gel

silica gel 60 matrix

Matrix

cross-linked agarose, 4%, spherical

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

agarose, 6%, spherical

Composite of cross-linked agarose and dextran

cross-linked, 6% agarose particles

Highly cross-linked 6% agarose

Highly cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Agarose, 4%

Cross-linked agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Cross-linked dextran

MDL Number

MFCD00005953

MFCD00011232

MFCD00075347

MFCD00132705

MFCD00132770

MFCD00145586

MFCD00145842

MFCD00148280

MFCD00149897

MFCD00165824

MFCD00166483

MFCD00211881

MFCD00677124

MFCD03098973

MFCD03791384

MFCD00000412

MFCD00009671

MFCD00132712

MFCD00132722

MFCD00216756

MFCD00011324

MFCD00011332

MFCD00071635

MFCD00084428

MFCD00132707

MFCD00132718

MFCD00132726

MFCD00132771

MFCD00145831

MFCD03096474

MFCD00000411

MFCD00008269

MFCD00078249

MFCD00081512

MFCD00132701

MFCD00135579

MFCD00145841

Melting Point

18.4°C

114°C

115°C

MFCD Number

MFCD00011232

MFCD00013066

MFCD00069903

MFCD00081512

Ligand

Recombinant Protein A (E.coli)

Ligand Concentration

~25 Âµmol phenyl/mL resin

4.5-8 mg gelatin/ml drained resin

~45 Âµmol phenyl/mL resin

~4mg heparin/mL resin

Magnesium Mg

10 ppb max.

Matrix

Highly cross-linked 4% agarose

6% Cross-Linked Agarose

Rigid, cross-linked agarose, spherical

Highly cross-linked agarose, spherical.

Molecular Formula

C2H7NO2

C7H16NNaO6S

CH5NO2

O2Si

C2H6OS

Molecular Weight

150.13

241.33

266.36

36.46

60.08

60.083

94.18

99.85

60.08

0.0

178.13 (anhydrous basis)

265.256

63.056

101.96

79.06

84.93

78.129

100.39

199.25

223.07

257.40

77.083

86.14

L CMSGradientSuitability

Pass Test

Lead Pb

10 ppb max.

Iron Fe

10 ppb max.

5ppm max.

Mesh Size

230-400 Mesh

Metal Ion Capacity

54 to 70 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

Name Note

microcrystalline

Nitrate

0.001% max.

No of Samples

10.0

Operating Flow Rate, Max

< 4 mL/min

1.0 mL/min

2.6 mL/min

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

Operating Flow Rate, Recommended

0.5 mL/min

0.04 mL/min

0.075 mL/min

1.3 mL/min

0.30 mL/min

Operating pH

0 - 14

0 - 7

4 - 14

Optical Activity

~7 (5% in H 2 O, suspension)

7.0 (5% in slurry)

~6.8 (10% in aq. suspension)

6.5 (5% in slurry)

Order code

SH30031.01

SH30237.01

SH30031.02

SH30268.01

SH30588.01

SH30588.02

SH30031.FS

Order Information

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

For research use only.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

Percoll PLUS is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Osmolality

288-310 mOsm/kg

276-298 mOsm/kg

< 30 mOsm/kg

Osmolality

322-344 mOsm/kg

< 25 mOsm/kg

P-CODES

P260

P314

P210-P260-P280-P301 + P310-P305 + P351 + P338-P311

P210-P280-P303 + P361 + P353-P304 + P340 + P310-P305 + P351 + P338 + P310-P370 + P378

P305 + P351 + P338

P210 - P302 + P352 - P305 + P351 + P338

P261 - P305 + P351 + P338

P280-P305 + P351 + P338-P310

P281

Pack size

100 g

2 x 1 ml 10% slurry

5 mL

50 mL

500 mL

1 Ã— 4.7 mL

1 Ã— 5 mL

1 mL

10 mL

150 mL

2 x 1 mL

200 mL

25 mL

250 mL

50 columns

500 g

6 mL

6 x 500 mL

1 L

1.5 g

1000 mL

5 Ã— 1 mL

6 x 1 L

1 Ã— 1 mL

1 piece

1.7 mL

100 mL

125 mL

20 mL

25 g

300 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

Packaging

Plastic Drum

Poly Bottle

Amber Glass

Glass Bottle

Manganese Mn

10 ppb max.

Packaging Layout

5 mL in glass bottle

500 g in poly drum

1 ft3 in fiber drum

100, 500 g in poly bottle

10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles

25 kg in fiber drum

25, 500 g in poly bottle

5, 25 kg in fiber drum

500 g in poly bottle

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

2.5, 5, 25 kg in fiber drum

Fibre case

2.5 kg in poly drum

250 g in poly bottle

5, 25, 100 mL in glass bottle

Partical Size Dry

18 Âµm-111 Âµm

>100 Âµm

>20 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

50 Âµm-150 Âµm

Particle Size

10 to 12 µm

40 to 63 µm

60 Âµm

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

35 to 70 µm

60/325 Mesh

80% between 60 and 100 Mesh

24-44 Âµm

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

60 to 80 mesh

Particle size distribution, dry

50 â€“ 150 Âµm

20 â€“ 80 Âµm

Particle Size in Methanol Max

163

Particle size, d_50V

~90 Âµm

~90 Î¼m

34 Âµm

~75 Âµm

~85 Âµm

~ 8.6 Âµm

~34 Âµm

~34 Î¼m

~50 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

pH stability, CIP

2â€“10

2â€“13

2â€“13.7

3â€“12

3â€“14

1â€“14

2â€“14

3â€“10

4â€“13

2â€“9

4â€“9

3â€“13

pH stability, operational

2â€“10

2â€“13

4â€“12

pH 6 to 10

3â€“10

2â€“9

3 to 12

3â€“11

3â€“13

4â€“13

4â€“9

2â€“12

3â€“12

3â€“9

Physical Form

Microcrystalline

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Liquid

Powder, -230+400 Mesh,S.A. 500-600m

Solid

Physical State

Liquid

liquidSuspension

Solid

Pore Size

0.7-0.85 cm 3 /g pore volume (approx.)

60 Å pore size

~0 cm 3 /g macroporosity

~0.55 mL/g pore volume

0.30 mL/g pore volume

0.8 cm 3 /g pore volume

60 +?

60 Å average pore size (52-73 Å)

60 Å mean pore size

60 Å medium pore diameter

0.75 cm 3 /g pore volume

0.75 mL/g pore volume

110 Å pore size

~0.8 cm 3 /g pore volume

0.43 cm 3 /g pore volume

0.5 mL/g pore volume

0.7-0.9 cm 3 /g pore volume

150 Å

30 Å

40 Å mean pore size

60 Å

90 Å mean pore size

Pressure/Flow Specification

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

Odor

Odorless

p H

7.5 pH

8.5 pH

Merck Index Reference

14,1965

Packaging

Bottle

Particle size distribution, wet

90â€“280 Âµm

100â€“300 Âµm

20â€“80 Âµm

15â€“88 Âµm

55â€“165 Âµm

87â€“510 Âµm

Particle Size in Methanol Min

ParticleSize

80/200 Mesh

Percent Purity

≥99.9%

>=97%

>=99%

7.0 to 7.4

7.2 - 7.5

7.2 pH

7.0

7.5 pH

6.0 to 7.0

8.5

pH ligand fully charged

Entire pH range

Above 6

Below 9

pH Range

5.5â€“7.5

8.5â€“9.5

8.9â€“9.9

Pictogram

GHS02, GHS05

GHS02, GHS06, GHS08

GHS07

GHS08

GHS02,GHS07

Pore Volume

0.8 mL/g

Potassium K

20 ppb max.

Pressure/flow characteristics

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Product Line

Pierce™

Pierce™, Tween™

Pierce, Tween

Tenax

Pierce

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Protease

Protease free

Pub Chem CID

24261

2723923

517165

23667519

679

>1600 C

Packaging Control

dry ice

PurityGrade Notes

Mobile phase for HPLC applications

Quality

regenerated

wet

monodispersed

dry

contains 1% TMCS

LiChropur ™

with fluorescent indicator 254 nm

pH Stability Cleaning-in-Place (CIP)

1 to 14

Nickel Ni

10 ppb max.

Quantity

1 L

10 g

100mL

200

100

100 g

50 g

75 cc

1/Pk.

Purity

99.8%

>=99%

>=97.0% (GC)

>=98.5% (excluding chlorotrimethylsilane, GC); 99% (excluding TMCS)

Purity Grade Notes

Also meets ACS specifications

Output

8 W

Particle SizeDistribution

5 to 20 µm

Quantity

100/Pk.

10 × 1 mL

2.5 kg

250 mL

5 × 1 mL

500 g

500g

1 kg

10 × 1.2 mL

500 mL

250g

5 kg

50g

Product Type

Blocking Buffer

R-CODES

36/37/38

11-23/24/25-39/23/24/25

10-36/37/38

11-34

48/20

10-34

Recommended Flow Rate

5 ml/min

1 ml/min

Recommended Storage

Ambient temperatures

Store at -20°C

Refractive Index

1.4770 to 1.4800 (at 25°C)

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Residue after Ignition

0.005% max.

Resin Type

MabSelect

Sepharose CL-4B

Sepharose High Performance

Capto

Sepharose Fast Flow

RIDADR

UN 2920 3(8) / PGII

UN 2924 8(3) / PGII

UN 3286 8(6.1)(3) / PGII

NONH for all modes of transport

UN 1993C 3 / PGIII

RTECS Number

FF5800000

VV7310000

FJ5691460

VV7315000

VV7565000

S-Codes

26-36

26-36/37/39-45

36/37

16-26-36/37/39-45

16-36/37-45

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Sample Volume

<= 13 ml

4 to 50 ÂµL

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

400 to 1000 Î¼l

<= 5 ml

100 to 300 Î¼l

Shelf Life

12 months

24 months

Signal Word

Warning

Danger

Silver Ag

10 ppb max.

SMILES

C(CNC(CO)(CO)CO)CS(=O)(=O)[O-].[Na+]

CC(=O)[O-].[NH4+]

C(=O)[O-].[NH4+]

CS(=O)C

O=[Si]=O

Residueafter Evaporation

5ppm max.

Resolution

40 to 63 µm

Scale

Lab|Process development

Lab

Lab|Process

Lab|Process development|Process

Stability

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Storage

-20C

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2 C -8 C

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 30Â°C, 0.2 M sodium acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

2-8C

2Â° to 8Â° C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°C to 8Â°C in 20% ethanol

Storage Conditions

At room temperature. Precipitation can occur at low temperature.

20% ethanol at 4Â°C to 30Â°C

4â€“30Â°C

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

Storage Requirements

-30°C to -10°C

Purity GradeNotes

Mobile phase for HPLC applications

ReagentType

Solvent (Pure)

Surface Area

~30 m 2 /g

250-350 m 2 /g

530 m 2 /g

550 m 2 /g

~500 m 2 /g

526 m 2 /g

120 m 2 /g

289 m 2 /g

380 m 2 /g

480 m 2 /g

500 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

BET surf. area ~500 m 2 /g

=480 m 2 /g (approx.)

~240 m 2 /g

175-225 m 2 /g

300 m 2 /g

430-530 m 2 /g

470-530 m 2 /g

Swelling factor

~1.7

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Synonym

azanium formate

ch3coonh4

DMSO

acetic acid, ammonium salt

aerosil

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Recommended maximum operational flow velocity

300 cm/h

Solubility Information

Soluble in hydrofluoric acid. Insoluble in water.

Soluble in water (333.33mg/ml).

Slightly soluble in sodium hydroxide. Insoluble in water,ethanol,ether and dilute mineral acids.

Solubility aq. soln.: insoluble.

Sorbent

Silica Gel 60 F254

Residue After Evaporation

5ppm max.

Residueafter Ignition

0.01% max.

Specific Surface

520 m2/g

Synonyms

Dowex(R) 1X8-200 Ion Exchange Resin

HPTLC (High Performance TLC) Aluminum Sheets

N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethylchlorosilane; N-Methyl-N-tert-butyldimethylsilyltrifluoroacetamide; MTBSTFA + 1% TBDMSCl; Silylating mixture VI

Silica gel 110 NH2

TLC Silica gel 60 W; TLC Silica gel 60 W, 25 Aluminium sheets 20 x 20 cm

XAD16N

MBTFA (N-methyl-bis(trifluoroacetamide)or BTFA (bis(TFA)); MBTFA

N-Methyl-N-(trimethylsilyl)trifluoroacetamide; N-Trimethylsilyl-N-methyl trifluoroacetamide; MSTFA

Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous

Silylating mixture V; BSTFA + TMCS

Amberlite(R) IR120 hydrogen form

Methanolic HCl

PLC Silica gel 60 F254, 1 mm; PLC Silica gel 60 F254, 1 mm, 15 Glass plates 20 x 20 cm

Activated magnesium silicate; Magnesium silicate

Silica gel

TLC cards Silica gel

TLC Silica gel 60 RP-18 F254s; TLC Silica gel 60 RP-18 F254s, 25 Glass plates 20 x 20 cm

TMSH

System

Sepax C-Pro|Sefia|Sepax 2

Sodium Na

50 ppb max.

Stokes Radius

~ 10

Size Metric TubeRack

12 g

Surface Area Metric

˜35 m2/g

Theoretical Plates

> 13000 m-1

5000 m-1

> 42 000 m^-1

> 48 000 m^-1

Stability

Stable

Thickness Metric

250 µm

200 µm

Sterilization

0.1 Âµm sterile filtered

TSCA

Yes

Sulfate

0.001% max.

Sulfate

0.005% max.

Target Organs

Respiratory system

Vapor Density

>1 (vs air)

<1 (vs air)

Vapor Pressure

8.8 mmHg ( 27 °C)

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

Viscosity

1.98 mPa/s at 25Â°C

Water

0.04% max.

2% max.

1% max.

Type

Classical Silica TLC Plate

Silica

Titratable Acid

0.001mEq/g max.

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Well Volume

8.5

WGK

nwg

Viscosity Max

Width Metric

2.5 cm

5 cm

Width Metric

4 cm

Wetted Part

Width English

1.57 in.

Zinc Zn

20 ppb max.

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