Percoll Plus density gradient media, 250 mL
For preparation of cells, subcellular particles and larger viruses. Percoll PLUS has verified low endotoxin level, and is nontoxic and resterilizable, even after adjustment to physiological ionic strength.
Gradients can either be preformed or spontaneously generated by centrifugation at moderate speeds in an anglehead rotor.
Isoosmotic gradients throughout cover a range of densities up to 1.3 g/ml.
Low viscosity allows cell isolations on preformed gradients in only a few minutes using low centrifugal forces (2NANA to 1NANANA x g).
Available in easytoopen, resealable 25NA ml and 1 l bottles.
Percoll PLUS is nontoxic, almost chemically inert, and does not adhere to membranes. Percoll PLUS can be stored unopened for at least five years. Preformed gradients can be stored for weeks without change in gradient shape, provided that the gradient remains unfrozen. Percoll PLUS can be buffered within the pH range 5.5 to 1NA without change in properties. Both concentrated and diluted Percoll PLUS can be resterilized by autoclaving for 3NA min at 12NA°C.