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Cytiva, now with the life sciences business from Pall Corporation, is a global biotechnology leader dedicated to helping customers discover and commercialize the next generation of therapeutics. Cytiva brings dedicated technical expertise and a broad portfolio of tools, technologies, and services that enable the development, manufacture, and delivery of transformative medicines to patients.

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Chromatography

Chromatography Consumables

Reagents and Buffers

Chromatography Media

$0~$10(0)
$10~$25(0)
$25~$50(0)
$50~$75(0)
$75~$100(1)
$100~$200(5)
$200~$300(18)
$300~$400(7)
$400~$500(13)
$500~$750(26)
$750~$1000(13)
$1000~$5000(54)
$5000~$10000(7)
$10000~$50000(0)
$50000~$100000(0)
$100000~$3000000(0)

Application

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

For purification of DNA from small molecules by gel filtration

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Application_4

Chromatography system

Cell isolation

Chromatography system|Manual

Bed Volume

5 mL

320

1 mL

Bed Dimensions

10 x 100 mm

6.4 x 100 mm

7 x 25 mm

16 x 100 mm

16 x 25 mm

26 x 600 mm

16 x 30 mm

Average sample loading capacity

50 mg

400 mg

Bead type

Mag Sepharose Magnetic Beads

Bed Height

600 mm

100 mm

30 mm

25 mm

Binding Capacity/Column

At least 10 mg histidine-tagged protein/ml sedimented medium.

Approx. 40 mg histidine-tagged protein/ml medium

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

BioProcess resin

yes

Binding Capacity/ml Chromatography Medium

> 25 mg horse liver GST/mL resin

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

At least 10 mg histidine-tagged protein

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

Application_5

Chromatography system|Manual

Certificate of Analysis

Yes

Chemical Stability

Stable in all commonly used aqueous buffers

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Commonly used aqueous buffers

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Stable in all commonly used buffer systems

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Stable in commonly used aqueous buffers, 1 M NaOH

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Chromatography technique

Affinity coupling

Ion Exchange Chromatography

Histidine-tagged protein purification

Antibody affinity chromatography.

Group specific affinity

Antibody affinity chromatography

GST-tagged protein purification

Hydrophobic Interaction Chromatography

Column i.d.

26 mm

6.4

16 mm

Density Max.

1.2 g/ml

1.135 g/ml|

1.078 g/ml

Color

White

Column volume

1 mL

8 mL

Color

Colorless to Slightly Yellow

Exclusion Limit Peptides

4 x 10Â³

Flow Rate

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

2 ml/min - 10 ml/min

< 20 mL/min

< DarcyÂ´s Law

Recommended: 0.5 to 2.0 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

< 20 mL/min |< 20 mL/min

1-4ml/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

>10 ml/min

>60 ml/min

< 5 ml/min

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 ml/min

> 4 ml/min

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

Flow Velocity

< 150 cm/h

<15 cm/h

‰¤ 600 cm/h|‰¤ 600 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

< 450 cm/h

< 450 cm/h|< 450 cm/h

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

< 500 cm/h

30-150cm/h

Estimated Shelf Life from Manufacture Date

8 years

5 years

3 years

Endotoxin Activity Max.

2 EU/ml

0.12 EU/mg

< 0.12 EU/ml

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Format_6

Bulk resin

Prepacked column

Prepacked columns

Ion Exchanger Type

Multimodal weak cation exchanger

Strong cation exchanger

Weak anion exchanger

Weak and strong anion

Weak cation exchanger

Strong anion exchanger

Weak anion

Ionic Capacity

0.13-0.17 mmol/ml

0.18-0.25 mmol H⁺/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.29-0.35 mmol Cl^-/ml medium

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.15-0.20 mmol H⁺/mlÂ

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

Ligand Coupling Method

Dextran surface extenders

Ether linkages

Ether linkages and hydrophilic spacer arm

Epoxy

Maleimide activation

Reductive Amination

Epoxy activation

Cyanogen bromide activation

Triazine

Ligand Density

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

~ 5 mg/mL resin

3 mg GammaBind G, type 3/ml medium

Approx. 13 Î¼mol/ml

3 mg Protein A/ml medium

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

Ligand_7

Protein A

Protein A, alkali stabilized

Protein L

Protein G

Butyl, octyl, phenyl

Matrix

Rigid, cross-linked agarose, spherical

Metal Ion Capacity

~15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

Operating Flow Rate, Max

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

< 4 mL/min

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

1.0 mL/min

2.6 mL/min

Osmolality

276-298 mOsm/kg

< 30 mOsm/kg

Pack size

1.5 g

5 Ã— 1 mL

6 x 1 L

2 x 1 ml 10% slurry

50 mL

1 L

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 columns

6 mL

15 g

10 mL

200 mL

25 mL

500 g

6 x 500 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1 Ã— 4.7 mL

10 g

2 x 1 mL

500 mL

1.7 mL

100 mL

125 mL

25 g

20 mL

300 mL

100 g

5 mL

Partical Size Dry

50 Âµm-150 Âµm

18 Âµm-111 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

>20 Âµm

>100 Âµm

Particle size, d_50V

~ 8.6 Âµm

~50 Âµm

~90 Âµm

34 Âµm

~100 Î¼m

~34 Âµm

~34 Î¼m

~85 Âµm

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~75 Âµm

~90 Î¼m

pH ligand fully charged

Below 9

Above 6

< 9

Entire pH range

pH stability, CIP

2â€“12

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

1â€“14

2â€“14

3â€“10

4â€“13

2â€“13

2â€“13.7

3â€“12

3â€“13

2â€“10

3â€“14

pH stability, operational

3 to 12

3â€“11

3â€“13

2â€“10

pH 6 to 10

3â€“10

2â€“9

4â€“13

4â€“9

2â€“12

3â€“12

3â€“9

2â€“13

4â€“12

Pressure/Flow Specification

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

Properties

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Sample Volume

25 to 500 ÂµL

400 to 1000 Î¼l

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

<= 13 ml

4 to 50 ÂµL

<= 5 ml

100 to 300 Î¼l

Group To be Coupled

Primary amines

-NHâ‚‚

Ligand

Alkaline-stabilized protein A-derived (E.coli).

Carboxymethyl

Native protein A

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Alkali-stabilized Protein A-derived (E. coli)

Diethylaminoethyl

Sulphopropyl

Porcine heparin

Recombinant protein A (E.coli)

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Cibacron Blue

Human IgG

Quaternary amine

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Matrix

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

agarose, 6%, spherical

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Cross-linked agarose, 4%

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Highly cross-linked agarose, spherical.

Composite of cross-linked agarose and dextran

Highly cross-linked 4% agarose

Highly cross-linked 6% agarose

cross-linked, 6% agarose particles

Highly cross-linked agarose

2% agarose

Cross-linked dextran

4% Agarose

4% cross-linked agarose

cross-linked agarose, 4%, spherical

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Includes

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Connectors for high- and low-pressure chromatography equipment and instructions.

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Ligand Concentration

~25 Âµmol phenyl/mL resin

~45 Âµmol phenyl/mL resin

4.5-8 mg gelatin/ml drained resin

~4mg heparin/mL resin

Licensing

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Order Information

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

For research use only.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

HiLoad columns are prepacked XK columns.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Percoll PLUS is for research use only.

Physical State

liquid

liquidSuspension

solid

Operating Flow Rate, Recommended

0.50 mL/min

0.04 mL/min

0.075 mL/min

0.80 mL/min

1.3 mL/min

0.75 mL/min

0.5 mL/min

0.30 mL/min

Osmolality

< 25 mOsm/kg

288-310 mOsm/kg

322-344 mOsm/kg

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Intrinsic Viscosity

~ 17

Recommended Flow Rate

5 ml/min

1 ml/min

Pack Size

pH Range

5.5â€“7.5

8.9â€“9.9

8.5â€“9.5

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Pressure/flow characteristics

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Particle Size

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

60 Î¼m-200 Î¼m

60 Âµm

24-44 Âµm

Particle size distribution, wet

100â€“300 Âµm

20â€“80 Âµm

90â€“280 Âµm

55â€“165 Âµm

87â€“510 Âµm

15â€“88 Âµm

pH Stability Cleaning-in-Place (CIP)

1 to 14

Storage

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 20% Ethanol

2 to 8Â°C, 100% Isopropanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

Storage Conditions

4â€“30Â°C

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

20% ethanol at 4Â°C to 30Â°C

Resin Type

MabSelect

Sepharose CL-4B

Capto

Sepharose Fast Flow

Sepharose High Performance

Particle Size in Methanol Min

Particle size distribution, dry

20 â€“ 80 Âµm

50 â€“ 150 Âµm

Stability

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Stable

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Particle Size in Methanol Max

163

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Scale

Lab|Process

Lab

Lab|Process development|Process

Lab|Process development

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Theoretical Plates

> 48 000 m^-1

> 13000 m-1

> 43 000 m^-1

5000 m-1

> 42 000 m^-1

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Recommended maximum operational flow velocity

300 cm/h

Viscosity Max

Wetted Part

Swelling factor

~1.7

Stokes Radius

~ 10

System

Sepax C-Pro|Sefia|Sepax 2

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Well Volume

8.5

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