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Chromatography

Chromatography Consumables

Reagents and Buffers

Buffers for Protein Methods

Specialized HPLC Reagents

Chromatography Media

Gas Chromatography Derivatization Reagents

Concentrated Buffers

Silylation Grade Solvents

Silylation Reagents

$0~$10(0)
$10~$25(3)
$25~$50(186)
$50~$75(53)
$75~$100(17)
$100~$200(89)
$200~$300(58)
$300~$400(28)
$400~$500(30)
$500~$750(47)
$750~$1000(31)
$1000~$5000(106)
$5000~$10000(8)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

Absorbance

0.02 AU max. at 330nm

Additives

Without: Calcium, magnesium, phenol red

Without: Phenol red

Without: Calcium, magnesium

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application

For purification of DNA from small molecules by gel filtration

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

Assay Percent Range

99.8% min by GC

Bed Dimensions

16 x 25 mm

26 x 600 mm

7 x 25 mm

16 x 30 mm

16 x 100 mm

10 x 100 mm

6.4 x 100 mm

Application_4

Cell isolation

Chromatography system|Manual

Chromatography system

Applications

ion chromatography: suitable

Assay

99.8%

=99%

Capacity

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

0.8 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

4.7 meq/g by dry weight

=1.1 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

1.7 meq/mL by wetted bed volume

Average sample loading capacity

50 mg

400 mg

Bed Height

100 mm

30 mm

600 mm

25 mm

Bed Volume

320

5 mL

1 mL

BioProcess resin

yes

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

Boiling Point

189°C

2230 C

Autoignition Temp

800 °F

~800 °F

Binding Capacity/ml Chromatography Medium

> 25 mg horse liver GST/mL resin

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

At least 10 mg histidine-tagged protein

Binding Capacity/Column

At least 10 mg histidine-tagged protein/ml sedimented medium.

Approx. 40 mg histidine-tagged protein/ml medium

Bead type

Mag Sepharose Magnetic Beads

Buffer

Running Buffers

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

CAS_NO

112945-52-5

9004-54-0

39339-85-0

104219-63-8

60177-39-1

62610-50-8

65099-79-8

69011-15-0

112926-00-8

1343-88-0

15461-27-5

9003-70-7

9041-35-4

9050-97-9

39389-20-3

63181-98-6

65546-95-4

75-09-2

7631-86-9

69011-20-7

11128-95-3

126850-14-4

1344-28-1

69011-19-4

Chem AlertStorageSymbol

Gray

Chemical Stability

Commonly used aqueous buffers

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Stable in all commonly used aqueous buffers

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Stable in all commonly used buffer systems

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Stable in commonly used aqueous buffers, 1 M NaOH

ChEBI

CHEBI:28262

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

Chemical Substance ID

24854645

24857358

24854191

329752189

24863799

24865203

329759181

24857354

24867159

329763462

24854194

24857352

329759188

24852120

24854190

329763461

24852837

24854823

329759183

329759193

329759187

329768868

329768201

329752001

24852304

24854187

329756032

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Application_5

Chromatography system|Manual

Chromatography technique

Ion Exchange Chromatography

Affinity coupling

Antibody affinity chromatography

GST-tagged protein purification

Histidine-tagged protein purification

Antibody affinity chromatography.

Group specific affinity

Hydrophobic Interaction Chromatography

Column i.d.

6.4

26 mm

16 mm

CAS Registry Number 1

67-68-5

Chemical Name or Material

Dimethylsulfoxide

Color

Colorless to Slightly Yellow

Density

1.100g/cmÂ³

Density Max.

1.135 g/ml|

1.2 g/ml

1.078 g/ml

Composition

Disodium Hydrogen Phosphate, Sodium Chloride

Tris Base, Sodium Chloride

nitrogen content, =1.0%

Concentration

10 X

1 N in NaOH (Regenerant concentration)

20 X

Decomposition Information

>190°C

Color

White

Colorless

Gold/Brown

Column volume

1 mL

8 mL

Content And Storage

Store at room temperature.

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

EC Number

215-681-1

238-878-4

231-545-4

215-710-8

231-717-9

215-691-6

200-838-9

COLOR

Blue

Endotoxin Activity Max.

2 EU/ml

< 0.12 EU/ml

0.12 EU/mg

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

Flow Rate

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

>10 ml/min

>60 ml/min

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

< 4 mL/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

2 ml/min - 10 ml/min

< 20 ml/min

< DarcyÂ´s Law

< 5 ml/min

Recommended: 0.5 to 2.0 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 20 mL/min |< 20 mL/min

1-4ml/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 mL/min

> 4 ml/min

Flow Velocity

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

< 500 cm/h

30-150cm/h

‰¤ 600 cm/h|‰¤ 600 cm/h

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

< 150 cm/h

<15 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 450 cm/h

< 450 cm/h|< 450 cm/h

Format_6

Prepacked column

Prepacked columns

Bulk resin

For Use With

GC/MS

Exclusion Limit Small Organics

5 x 10Â³

Flashpoint (°C)

> 240 C

Field14

50Â°-65Â°

For Use With

For all Biacore SPR systems.

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

Form

fine powder

fine crystals

powder

liquid

powder or crystals

beads

Formula

(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃

Al2O3

O2Si

MgO₃Si

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

SiO₂

CH2Cl2

Service & Support

ZrOCl₂·xH₂O

Estimated Shelf Life from Manufacture Date

8 years

3 years

5 years

Gel Compatibility

Novex® Tris-Glycine Gels, Tris-Glycine Gels, Precise Tris-Glycine Gels

Empirical Formula

O2Si

MgO3Si

Feature

ferrule type packed column

Flash Point

87°C (188.6°F)

Exclusion Limit Peptides

4 x 10Â³

Format

matrix material

Liquid

Formula Weight

100.39

60.08

78.13

Gel Type

Tris-Glycine Gel

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Grade

certified reference material

high-purity grade (Davisil Grade 636)

nuclear grade

reagent grade

Standard

high-purity grade (7754)

technical grade

high-purity grade (Davisil Grade 643)

HPLC

For column chromatography

high-purity grade (Davisil Grade 646)

Group To be Coupled

Primary amines

-NHâ‚‚

Grade

High-purity Grade (Davisil Grade 923)

High-purity Grade (Davisil Grade 633)

High-purity Grade

High-purity Grade (Davisil Grade 62)

H-Codes

H372

H319

H373

H371

Ion Exchanger Type

Multimodal weak cation exchanger

Strong cation exchanger

Weak and strong anion

Weak anion exchanger

Weak cation exchanger

Strong anion exchanger

Weak anion

Ionic Capacity

0.13-0.17 mmol/ml

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.15-0.20 mmol H⁺/mlÂ

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.29-0.35 mmol Cl^-/ml medium

0.18-0.25 mmol H⁺/ml medium

Hazard s

Flammable, Irritant

Health Hazard 2

WARNING!

Ligand Coupling Method

Dextran surface extenders

Ether linkages and hydrophilic spacer arm

Reductive Amination

Cyanogen bromide activation

Triazine

Epoxy activation

Ether linkages

Epoxy

Maleimide activation

Ligand Density

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

3 mg Protein A/ml medium

3 mg GammaBind G, type 3/ml medium

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

Approx. 13 Î¼mol/ml

~ 5 mg/mL resin

Ligand_7

Protein A

Protein A, alkali stabilized

Butyl, octyl, phenyl

Protein G

Protein L

Matrix

Rigid, cross-linked agarose, spherical

Highly Cross-Linked Agarose

Identification

Pass Test

MDL Number

MFCD00132718

MFCD00132726

MFCD00145831

MFCD03096474

MFCD00132707

MFCD00132771

MFCD00145841

MFCD00011232

MFCD00145586

MFCD00084428

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00149897

MFCD00211881

MFCD03791384

MFCD00132712

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00132722

MFCD00145842

MFCD00165824

MFCD00166483

Metal Ion Capacity

~15 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

Includes

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Connectors for high- and low-pressure chromatography equipment and instructions.

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand

Diethylaminoethyl

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Porcine heparin

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Cibacron Blue

Human IgG

Quaternary amine

Native protein A

Sulphopropyl

Alkali-stabilized Protein A-derived (E. coli)

Alkaline-stabilized protein A-derived (E.coli).

Carboxymethyl

Ligand Concentration

~45 Âµmol phenyl/mL resin

~4mg heparin/mL resin

4.5-8 mg gelatin/ml drained resin

~25 Âµmol phenyl/mL resin

Matrix

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Highly cross-linked agarose, spherical.

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

cross-linked, 6% agarose particles

2% agarose

Cross-linked dextran

4% Agarose

4% cross-linked agarose

cross-linked agarose, 4%, spherical

Composite of cross-linked agarose and dextran

Highly cross-linked 4% agarose

Highly cross-linked 6% agarose

Cross-linked agarose, 4%

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

agarose, 6%, spherical

IUPAC Name

methylsulfinylmethane

Ligand

Recombinant Protein A (E.coli)

Intrinsic Viscosity

~ 17

IOD-Codes

Linear Formula

MgO3Si

(C10H12 · C10H10 · C8H8)x

(C10H12 · C10H10 · C8H8 · C3H9N)X

SiO2

(CH₃)₂SO

Material

Plastic/Stainless Steel

N/A

PEEK

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Melting Point

18.4°C

Molecular Weight

100.39

101.96

0.0

60.08

178.13 (anhydrous basis)

60.08

84.93

78.129

Order Information

Percoll PLUS is for research use only.

For research use only.

HiLoad columns are prepacked XK columns.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

Pack size

1.7 mL

100 mL

125 mL

25 g

1 L

1 Ã— 4.7 mL

10 g

2 x 1 mL

1 piece

20 mL

300 mL

100 g

5 mL

1000 mL

15 g

500 mL

10 mL

200 mL

25 mL

4 x 50 mL

500 g

6 x 500 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 columns

6 mL

2 x 1 ml 10% slurry

50 mL

1.5 g

5 Ã— 1 mL

6 x 1 L

Packaging Layout

2.5 kg in poly drum

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

1 ft3 in fiber drum

25 kg in fiber drum

25, 500 g in poly bottle

2.5, 5, 25 kg in fiber drum

500 g in poly drum

100, 500 g in poly bottle

5, 25 kg in fiber drum

10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles

500 g in poly bottle

Particle Size

24-44 Âµm

60 Î¼m-200 Î¼m

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

60 Âµm

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

Particle size distribution, wet

15â€“88 Âµm

100â€“300 Âµm

20â€“80 Âµm

90â€“280 Âµm

55â€“165 Âµm

87â€“510 Âµm

pH Range

8.5â€“9.5

5.5â€“7.5

8.9â€“9.9

pH stability, CIP

3â€“13

3â€“10

4â€“13

2â€“10

3â€“14

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

2â€“13

2â€“13.7

3â€“12

1â€“14

2â€“14

2â€“12

pH stability, operational

2â€“12

3â€“12

3â€“9

2â€“13

4â€“12

2â€“9

4â€“13

4â€“9

3â€“10

2â€“10

pH 6 to 10

3 to 12

3â€“11

3â€“13

Kit Contents

BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL

Pore Size

0.5 mL/g pore volume

0.7-0.9 cm 3 /g pore volume

30 Å

110 Å pore size

~0.55 mL/g pore volume

150 Å

90 Å mean pore size

0.75 cm 3 /g pore volume

0.7-0.85 cm 3 /g pore volume (approx.)

60 Å mean pore size

0.30 mL/g pore volume

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

~0.8 cm 3 /g pore volume

>1600 C

Operating Flow Rate, Max

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

1.0 mL/min

2.6 mL/min

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

< 4 mL/min

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

Operating Flow Rate, Recommended

0.50 mL/min

0.75 mL/min

0.30 mL/min

1.3 mL/min

0.5 mL/min

0.04 mL/min

0.075 mL/min

0.80 mL/min

Operating pH

0 - 7

0 - 14

Osmolality

< 25 mOsm/kg

322-344 mOsm/kg

288-310 mOsm/kg

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Partical Size Dry

20 Âµm-50 Âµm

40 Âµm-120 Âµm

>100 Âµm

>20 Âµm

18 Âµm-111 Âµm

50 Âµm-150 Âµm

Particle size, d_50V

~100 Î¼m

~34 Âµm

~34 Î¼m

~75 Âµm

~90 Î¼m

~85 Âµm

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~90 Âµm

34 Âµm

~ 8.6 Âµm

~50 Âµm

pH ligand fully charged

Above 6

< 9

Entire pH range

Below 9

Pictogram

GHS07

GHS08

Physical State

Solid

liquidSuspension

liquid

Pressure/Flow Specification

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

Pressure/flow characteristics

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Product Line

Pierce, Tween

Pierce

Pierce™

Pierce™, Tween™

Recommended maximum operational flow velocity

300 cm/h

Residue After Evaporation

5ppm max.

Resin Type

Capto

Sepharose Fast Flow

Sepharose High Performance

MabSelect

Sepharose CL-4B

RIDADR

NONH for all modes of transport

Order code

SH30237.01

SH30588.02

SH30031.02

SH30268.01

SH30588.01

SH30031.FS

SH30031.01

Optical Activity

6.5 (5% in slurry)

7.0 (5% in slurry)

~7 (5% in H 2 O, suspension)

P-CODES

P260

P314

P305 + P351 + P338

Particle size distribution, dry

50 â€“ 150 Âµm

20 â€“ 80 Âµm

Particle Size in Methanol Max

163

pH Stability Cleaning-in-Place (CIP)

1 to 14

Quantity

500 g

10 x 1 mL ampules

50 g

100 g

500 mL

1 L

Molecular Formula

C2H6OS

R-CODES

36/37/38

48/20

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Sample Volume

400 to 1000 Î¼l

<= 5 ml

100 to 300 Î¼l

25 to 500 ÂµL

<= 13 ml

4 to 50 ÂµL

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Scale

Lab

Lab|Process development|Process

Lab|Process development

Lab|Process

Sterilization

0.1 Âµm sterile filtered

Storage

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2-8C

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

2Â° to 8Â° C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol

2 to 8Â°C, 100% Isopropanol

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

Storage Conditions

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

At room temperature. Precipitation can occur at low temperature.

4â€“30Â°C

20% ethanol at 4Â°C to 30Â°C

Store at room temperature.

Surface Area

175-225 m 2 /g

550 m 2 /g

380 m 2 /g

500 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

300 m 2 /g

470-530 m 2 /g

120 m 2 /g

289 m 2 /g

480 m 2 /g

~30 m 2 /g

250-350 m 2 /g

530 m 2 /g

=480 m 2 /g (approx.)

430-530 m 2 /g

~500 m 2 /g

p H

8.5 pH

7.5 pH

Particle Size in Methanol Min

PurityGrade Notes

Mobile phase for HPLC applications

Quality

regenerated

monodispersed

dry

wet

Purity

>=99%

99.8%

Stability

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

StandardComponents

MSTFA (N-Methyl-N-trimethylsilyltrifluoroacetamide)

Osmolality

< 30 mOsm/kg

276-298 mOsm/kg

Residueafter Evaporation

5ppm max.

S-Codes

26-36

Shelf Life

24 months

12 Months

Pack Size

Packaging

Amber glass

Percent Purity

≥99.9%

7.0 to 7.4

7.2 - 7.5

7.2 pH

7.5 pH

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Liquid

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Signal Word

Danger

Warning

SMILES

CS(=O)C

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Quantity

RTECS Number

VV7315000

VV7310000

Standard Concentration

98 to 100%

Pub Chem CID

679

Stokes Radius

~ 10

Recommended Flow Rate

5 ml/min

1 ml/min

Purity GradeNotes

Mobile phase for HPLC applications

Refractive Index

1.4770 to 1.4800 (at 25°C)

Swelling factor

~1.7

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

WGK

nwg

Synonyms

Amberlite(R) IR120 hydrogen form

Activated magnesium silicate; Magnesium silicate

Silica gel

Silica gel 110 NH2

Dowex(R) 1X8-200 Ion Exchange Resin

XAD16N

Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous

Synonym

DMSO

Theoretical Plates

> 48 000 m^-1

> 43 000 m^-1

5000 m-1

> 42 000 m^-1

> 13000 m-1

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

Titratable Acid

0.001mEq/g max.

Storage Spec

2°C to 8°C

System

Sepax C-Pro|Sefia|Sepax 2

Type of ion exchanger

Strong anion exchanger (quaternary ammonium)

Strong cation exchanger (methyl sulfonate)

Vapor Density

<1 (vs air)

>1 (vs air)

Viscosity Max

Water

0.04% max.

Viscosity

1.98 mPa/s at 25Â°C

Wetted Part

Well Volume

8.5

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