Government Scientific Source

$0~$10(0)
$10~$25(1)
$25~$50(194)
$50~$75(42)
$75~$100(18)
$100~$200(70)
$200~$300(49)
$300~$400(18)
$400~$500(29)
$500~$750(38)
$750~$1000(21)
$1000~$5000(62)
$5000~$10000(2)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

Absorbance

Pass Test

Absorbance

0.02 AU max. at 330nm

Additives

Without: Calcium, magnesium, phenol red

Without: Phenol red

Without: Calcium, magnesium

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Aluminum Al

20 ppb max.

Application

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

For the removal and/or purification of serine proteases e.g. thrombin

Application_4

Cell isolation

Chromatography system|Manual

Assay Percent Range

>=99 %

99.8% min by GC

>=97 %

Autoignition Temp

800 °F

Bed Height

100 mm

30 mm

600 mm

25 mm

Bed Dimensions

16 x 25 mm

7 x 25 mm

16 x 100 mm

16 x 30 mm

Bed Volume

5 mL

320

1 mL

Application_5

Chromatography system|Manual

Assay

=99%

Assay Percent Notes

99% min

Bead type

Mag Sepharose Magnetic Beads

Calcium Ca

50 ppb max.

Capacity

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

0.8 meq/mL by wetted bed volume

3.8 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

1.7 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

4.7 meq/g by dry weight

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

Binding Capacity/ml Chromatography Medium

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

At least 10 mg histidine-tagged protein

BioProcess resin

yes

Boiling Point

130-132 C (lit.)

189°C

2230 C

45-50 C/14 mmHg (lit.)

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

CAS Registry Number 1

631-61-8

540-69-2

91000-53-2

67-68-5

7631-86-9

9004-34-6

CAS_NO

112926-00-8

1343-88-0

161308-36-7

17287-03-5

2042-27-5

112945-52-5

373-57-9

25561-30-2

7631-86-9

110-85-0

39339-85-0

9003-70-7

9041-35-4

9050-97-9

15461-27-5

7647-01-0

77377-52-7

9004-34-6

9004-54-0

9052-45-3

39389-20-3

63181-98-6

65546-95-4

69011-20-7

75-09-2

11128-95-3

126850-14-4

1344-28-1

24589-78-4

60177-39-1

62610-50-8

65099-79-8

69011-15-0

69011-19-4

Buffer

Running Buffers

ChEBI

CHEBI:28262

CHEBI:30563

CHEBI:62947

CHEBI:63050

Chem AlertStorageSymbol

Gray

Certificate of Analysis

Yes

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chem Alert Storage Symbol

Gray

Chemical Name or Material

Ammonium Acetate

Cellulose

Dimethylsulfoxide

Ammonium Formate

Silica Gel 60

Silica Gel

TAPS buffer soln.

Chemical Stability

Commonly used aqueous buffers

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in all commonly used buffer systems

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Stable in commonly used aqueous buffers, 1 M NaOH

Chemical Substance ID

24852120

24854194

24854190

24857352

24863799

24865203

24848188

24852837

24854191

329750793

24854823

329752001

24857354

329759181

329759188

24867159

329763461

329770161

329752189

24852304

329759183

329759193

24854187

24854645

24857358

329766419

329768201

24898556

329817869

329756032

329759187

329763462

329768868

329817850

Chromatography technique

Ion Exchange Chromatography

Hydrophobic Interaction Chromatography

Histidine-tagged protein purification

Antibody affinity chromatography

Antibody affinity chromatography.

Group specific affinity

GST-tagged protein purification

Chloride

0.0005% max.

0.001% max.

5ppm max.

Color

Colorless

Color

Colorless to Slightly Yellow

Gold/Brown

Column i.d.

6.4

26 mm

Column Type

Silica

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Composition

Disodium Hydrogen Phosphate, Sodium Chloride

nitrogen content, =1.0%

Tris Base, Sodium Chloride

Concentration

~0.25 M in methanol

20 X

3 M in methanol

0.2M buffer soln.

10 X

Concentration

3% in PBS

1 N in NaOH (Regenerant concentration)

COLOR

White

Blue

Content AndStorage

Store between 2 to 25°C in a dry, cool, well-ventilated area, away from incompatible materials.

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.

Copper Cu

10 ppb max.

Decomposition Information

>180°C

>190°C

Density

˜0.25 g/mL (free fall density)

Density

0.871-0.881 g/mL at 20 °C

0.97 g/mL (lit.)

1.100g/cmÂ³

1.075 g/mL at 25 °C (lit.)

Description

1 Chr cellulose chromatography roll is a 0.18 mm paper for general analytical separations.

Content And Storage

Store at room temperature.

Density Max.

1.135 g/ml|

1.2 g/ml

1.078 g/ml

EC Number

200-838-9

203-808-3

215-691-6

215-710-8

231-545-4

231-717-9

215-681-1

238-878-4

232-674-9

EINECS Number

231-545-4

Empirical Formula

C3H10OS

C6H12F3NOSi

MgO3Si

C8H18F3NOSi2

C9H18F3NOSi

O2Si

Endotoxin Activity Max.

0.12 EU/mg

< 0.12 EU/ml

2 EU/ml

Estimated Shelf Life from Manufacture Date

3 years

5 years

Exclusion Limit Peptides

4 x 10Â³

Feature

No Flourescent Indicator

ferrule type packed column

Film Thickness

0.18 mm

Filtration Test

Pass Test

Flashpoint (°C)

> 240 C

45 C - closed cup

11 C

6 C

Flow Rate

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

< 4 mL/min

< 4 mL/min |< 4 mL/min

< 4 ml/min|< 4 ml/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 1 ml/min|< 1 ml/min

< 20 ml/min|< 20 ml/min

> 4 ml/min

>60 ml/min|> 60 ml/min

< 1.0 ml/min

>10 ml/min

< 20 mL/min

< DarcyÂ´s Law

Flash Point

87°C (188.6°F)

Flow Velocity

< 150 cm/h

< 500 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

30-150cm/h

< 130 cm/h

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 450 cm/h

< 450 cm/h|< 450 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 300 cm/h|< 300 cm/h

< 60 cm/hour

<1800 cm/h

For Use With

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

Dimensions LxW

4.0 cm x 100 m (1.57 in. x 328.0 ft.)

For Use With Application

Basic Applications, Polar Compounds

Column Chromatography

Form

fine crystals

neat

powder or crystals

fine powder

powder

Form

Liquid

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Format

matrix material

Format_6

Prepacked column

Prepacked columns

Bulk resin

Formula

(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃

MgO₃Si

Service & Support

O2Si

Format

Liquid

Formula Weight

100.39

78.13

60.08

77.08

Grade

High-purity Grade (Davisil Grade 643)

High-purity Grade (Davisil Grade 923)

633

High-purity Grade (Davisil Grade 62)

Technical Grade

Grade 1 Chr

Nuclear Grade

Reagent Grade

High-purity Grade (7754)

Grade

high-purity grade (Davisil Grade 646)

HPLC

for column chromatography

High-Purity Grade

analytical standard

certified reference material

for GC derivatization

high-purity grade (Davisil Grade 633)

high-purity grade (Davisil Grade 636)

Standard

H-Codes

H225-H314

H226 - H315 - H319 - H335

H319

H373

H225-H301-H311-H315-H319-H331-H370

H371

H372

Health Hazard 2

CAUTION!

GHS H Statement
H335

WARNING!

Health Hazard 1

H373: May cause damage to organs through prolonged or repeated exposure.

H500

Health Hazard 3

GHS P Statement
P261-P271-P304+P340-P312-P405-P403+P233-P501a

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Emergency Overview
May cause skin, eye, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if s

Identification

Pass Test

Heavy MetalsasPb

5ppm max.

Ignition Residue

0.01% max.

InChIKey

FEGYIWVHCSRXCG-UHFFFAOYSA-M

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

USFZMSVCRYTOJT-UHFFFAOYSA-N

VYPSYNLAJGMNEJ-UHFFFAOYSA-N

VZTDIZULWFCMLS-UHFFFAOYSA-N

Includes

Connectors for high- and low-pressure chromatography equipment and instructions.

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Ionic Capacity

0.15-0.20 mmol H⁺/mlÂ

0.18-0.25 mmol H⁺/ml medium

0.29-0.35 mmol Cl^-/ml medium

0.07-0.09 mmol H⁺/ml medium

0.09-0.13 mmol H⁺/ml medium

0.11â€“0.16 mmol (Cl^-)/mL resin

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

3-4 mmol/g

0.13-0.17 mmol/ml

0.18-0.24 mmol Cl^-/ml medium

Includes

18 Pc.

IUPAC Name

azanium;acetate

azanium;formate

sodium;3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonate

dioxosilane

methylsulfinylmethane

Insoluble Matter

0.005% max.

Intrinsic Viscosity

~ 17

IOD-Codes

F,T

F,C

Ion Exchanger Type

Strong anion exchanger

Weak anion

Weak and strong anion

Weak cation exchanger

Multimodal weak cation exchanger

Strong cation exchanger

Weak anion exchanger

Ligand

Carboxymethyl

Porcine heparin

Sulphopropyl

Alkaline-stabilized protein A-derived (E.coli).

Bovine gelatin

Cibacron Blue

Glutathione and 10-carbon linker arm

Human IgG

Phenyl

Quaternary amine

Alkali-stabilized Protein A-derived (E. coli)

Diethylaminoethyl

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Calmodulin

Native Protein A

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Ligand

Recombinant Protein A (E.coli)

Ligand Concentration

~25 Âµmol phenyl/mL resin

4.5-8 mg gelatin/ml drained resin

~45 Âµmol phenyl/mL resin

~4mg heparin/mL resin

Ligand Coupling Method

Epoxy

Maleimide activation

Cyanogen bromide activation

Epoxy activation

Triazine

Reductive Amination

Dextran surface extenders

Ether linkages

Ether linkages and hydrophilic spacer arm

Ligand Density

4.5-8 mg gelatin/ml drained resin

~ 5 mg/mL resin

3 mg Protein A/ml medium

Approx. 13 Î¼mol/ml

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

3 mg GammaBind G, type 3/ml medium

Ligand_7

Protein L

Butyl, octyl, phenyl

Protein A

Protein A, alkali stabilized

Protein G

Linear Formula

SiO2

(CH3)3S(OH)

C9H18F3NOSi

(C10H12 · C10H10 · C8H8)x

(CH₃)₂SO

CF3C[=NSi(CH3)3]OSi(CH3)3

CH3COONH4

HCl

(C10H12 · C10H10 · C8H8 · C3H9N)X

CF3CON(CH3)Si(CH3)3

HCO2NH4

MgO3Si

Magnesium Mg

10 ppb max.

Iron Fe

10 ppb max.

5ppm max.

Matrix

agarose, 6%, spherical

Composite of cross-linked agarose and dextran

cross-linked, 6% agarose particles

Highly cross-linked 6% agarose

Highly cross-linked agarose

cross-linked agarose, 4%, spherical

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

Agarose, 4%

Cross-linked agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Cross-linked dextran

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Matrix

Highly cross-linked 4% agarose

Highly cross-linked agarose, spherical.

6% Cross-Linked Agarose

Rigid, cross-linked agarose, spherical

MDL Number

MFCD00009671

MFCD00132712

MFCD00132722

MFCD00216756

MFCD00005953

MFCD00011232

MFCD00075347

MFCD00132770

MFCD00145586

MFCD00145842

MFCD00148280

MFCD00149897

MFCD00165824

MFCD00166483

MFCD00211881

MFCD00677124

MFCD03098973

MFCD00000411

MFCD03791384

MFCD00008269

MFCD00078249

MFCD00081512

MFCD00132701

MFCD00135579

MFCD00145841

MFCD00011324

MFCD00071635

MFCD00084428

MFCD00132707

MFCD00132718

MFCD00132726

MFCD00132771

MFCD03096474

Length Metric

100 m

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

Material

Glass Support

Polypropylene

Alumina

Cellulose

N/A

Silica gel

Florisil

PEEK

Plastic/Stainless Steel

Melting Point

114°C

18.4°C

115°C

L CMSGradientSuitability

Pass Test

Lead Pb

10 ppb max.

Manganese Mn

10 ppb max.

Mesh Size

230-400 Mesh

Metal Ion Capacity

54 to 70 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

MFCD Number

MFCD00081512

MFCD00011232

MFCD00013066

MFCD00069903

Molecular Formula

C7H16NNaO6S

CH5NO2

C2H7NO2

C2H6OS

O2Si

Molecular Weight

60.08

0.0

178.13 (anhydrous basis)

265.256

63.056

150.13

241.33

266.36

36.46

60.08

60.083

94.18

99.85

100.39

199.25

101.96

257.40

84.93

77.083

86.14

78.129

Merck Index Reference

14,1965

Odor

Odorless

Operating Flow Rate, Max

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

< 4 mL/min

1.0 mL/min

2.6 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

Operating Flow Rate, Recommended

0.04 mL/min

0.075 mL/min

0.5 mL/min

0.30 mL/min

1.3 mL/min

Order code

SH30031.02

SH30268.01

SH30588.01

SH30031.01

SH30237.01

SH30031.FS

SH30588.02

p H

7.5 pH

8.5 pH

P-CODES

P210-P260-P280-P301 + P310-P305 + P351 + P338-P311

P210-P280-P303 + P361 + P353-P304 + P340 + P310-P305 + P351 + P338 + P310-P370 + P378

P260

P314

P210 - P302 + P352 - P305 + P351 + P338

P261 - P305 + P351 + P338

P305 + P351 + P338

Pack size

1 Ã— 4.7 mL

1 Ã— 5 mL

1 mL

10 mL

150 mL

2 x 1 mL

200 mL

25 mL

250 mL

50 columns

500 g

6 mL

6 x 500 mL

100 g

2 x 1 ml 10% slurry

5 mL

50 mL

500 mL

1 Ã— 1 mL

1 piece

1.7 mL

100 mL

125 mL

20 mL

25 g

300 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1 L

1.5 g

1000 mL

5 Ã— 1 mL

6 x 1 L

Packaging

Amber glass

Bottle

Glass Bottle

Plastic Drum

Poly Bottle

Packaging Layout

1 ft3 in fiber drum

100, 500 g in poly bottle

10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles

25 kg in fiber drum

25, 500 g in poly bottle

5, 25 kg in fiber drum

500 g in poly bottle

500 g in poly drum

2.5 kg in poly drum

5, 25, 100 mL in glass bottle

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

2.5, 5, 25 kg in fiber drum

Partical Size Dry

>100 Âµm

18 Âµm-111 Âµm

>20 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

50 Âµm-150 Âµm

Particle size distribution, wet

90â€“280 Âµm

15â€“88 Âµm

55â€“165 Âµm

87â€“510 Âµm

100â€“300 Âµm

20â€“80 Âµm

Particle Size in Methanol Min

Particle size, d_50V

~75 Âµm

~85 Âµm

~90 Âµm

~90 Î¼m

34 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~ 8.6 Âµm

~34 Âµm

~34 Î¼m

~50 Âµm

ParticleSize

80/200 Mesh

Percent Purity

≥99.9%

>=97%

>=99%

7.0 to 7.4

7.2 - 7.5

7.2 pH

6.0 to 7.0

8.5

7.0

7.5 pH

pH ligand fully charged

Entire pH range

Above 6

Below 9

pH Range

5.5â€“7.5

8.5â€“9.5

8.9â€“9.9

pH stability, CIP

1â€“14

2â€“14

3â€“10

4â€“13

2â€“10

2â€“13

2â€“13.7

3â€“12

3â€“14

3â€“13

2â€“9

4â€“9

pH stability, operational

3â€“10

2â€“10

2â€“13

4â€“12

pH 6 to 10

2â€“12

3â€“12

3â€“9

2â€“9

3 to 12

3â€“11

3â€“13

4â€“13

4â€“9

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Microcrystalline

Solid

Liquid

Powder, -230+400 Mesh,S.A. 500-600m

Physical State

liquidSuspension

Solid

Liquid

Pictogram

GHS02, GHS05

GHS02, GHS06, GHS08

GHS02,GHS07

GHS07

GHS08

>1600 C

Pore Size

0.30 mL/g pore volume

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

60 Å mean pore size

60 Å medium pore diameter

0.7-0.85 cm 3 /g pore volume (approx.)

~0.8 cm 3 /g pore volume

0.7-0.9 cm 3 /g pore volume

150 Å

30 Å

90 Å mean pore size

0.75 cm 3 /g pore volume

110 Å pore size

Potassium K

20 ppb max.

Pressure/flow characteristics

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/Flow Specification

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

Product Line

Pierce, Tween

Pierce

Tenax

Pierce™

Pierce™, Tween™

Name Note

microcrystalline

Nitrate

0.001% max.

Operating pH

0 - 14

4 - 14

0 - 7

Optical Activity

~7 (5% in H 2 O, suspension)

7.0 (5% in slurry)

6.5 (5% in slurry)

Nickel Ni

10 ppb max.

Order Information

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Percoll PLUS is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

For research use only.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

Osmolality

288-310 mOsm/kg

322-344 mOsm/kg

< 25 mOsm/kg

Osmolality

< 30 mOsm/kg

276-298 mOsm/kg

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Protease

Protease free

Particle Size

40 to 63 µm

60 Âµm

24-44 Âµm

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

60 to 80 mesh

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

35 to 70 µm

60/325 Mesh

80% between 60 and 100 Mesh

Particle size distribution, dry

50 â€“ 150 Âµm

20 â€“ 80 Âµm

Particle Size in Methanol Max

163

Pub Chem CID

24261

2723923

517165

23667519

679

Packaging Control

dry ice

Purity

99.8%

>=98.5% (excluding chlorotrimethylsilane, GC); 99% (excluding TMCS)

>=99%

Purity Grade Notes

Also meets ACS specifications

Quality

monodispersed

contains 1% TMCS

LiChropur ™

regenerated

wet

dry

pH Stability Cleaning-in-Place (CIP)

1 to 14

Quantity

100 g

10 g

Quantity

10 × 1 mL

250 mL

5 × 1 mL

50 g

500 g

500g

1 L

250g

5 kg

100mL

2.5 kg

50g

1 kg

10 × 1.2 mL

500 mL

R-CODES

11-23/24/25-39/23/24/25

36/37/38

10-36/37/38

11-34

48/20

Product Size

2.5kg

Product Type

Blocking Buffer

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Residue after Ignition

0.005% max.

Resin Type

MabSelect

Sepharose CL-4B

Sepharose High Performance

Capto

Sepharose Fast Flow

RTECS Number

VV7310000

FJ5691460

VV7315000

VV7565000

S-Codes

26-36

16-26-36/37/39-45

16-36/37-45

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Sample Volume

<= 13 ml

4 to 50 ÂµL

<= 5 ml

100 to 300 Î¼l

400 to 1000 Î¼l

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

Shelf Life

12 months

24 months

Signal Word

Warning

Danger

Silver Ag

10 ppb max.

SMILES

C(CNC(CO)(CO)CO)CS(=O)(=O)[O-].[Na+]

CC(=O)[O-].[NH4+]

C(=O)[O-].[NH4+]

O=[Si]=O

CS(=O)C

Purity GradeNotes

Mobile phase for HPLC applications

PurityGrade Notes

Mobile phase for HPLC applications

Stability

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Storage

-20C

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2 C -8 C

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°C to 8Â°C in 20% ethanol

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

2-8C

2Â° to 8Â° C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

4 to 8Â°C, 20% Ethanol

Storage Conditions

At room temperature. Precipitation can occur at low temperature.

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

4â€“30Â°C

20% ethanol at 4Â°C to 30Â°C

Storage Requirements

-30°C to -10°C

Surface Area

~30 m 2 /g

250-350 m 2 /g

530 m 2 /g

550 m 2 /g

=480 m 2 /g (approx.)

175-225 m 2 /g

300 m 2 /g

430-530 m 2 /g

470-530 m 2 /g

120 m 2 /g

289 m 2 /g

480 m 2 /g

500 m 2 /g

500 m 2 /g (approx.)

~500 m 2 /g

Swelling factor

~1.7

Recommended Flow Rate

1 ml/min

5 ml/min

Recommended maximum operational flow velocity

300 cm/h

Recommended Storage

Store at -20°C

Ambient temperatures

Residue After Evaporation

5ppm max.

Residueafter Ignition

0.01% max.

RIDADR

NONH for all modes of transport

UN 1993C 3 / PGIII

UN 3286 8(6.1)(3) / PGII

UN 2924 8(3) / PGII

Refractive Index

1.4770 to 1.4800 (at 25°C)

ReagentType

Solvent (Pure)

Scale

Lab

Lab|Process

Lab|Process development|Process

Lab|Process development

Synonym

azanium formate

acetic acid, ammonium salt

aerosil

ch3coonh4

DMSO

Size Metric TubeRack

12 g

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Residueafter Evaporation

5ppm max.

Resolution

40 to 63 µm

Synonyms

Dowex(R) 1X8-200 Ion Exchange Resin

N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethylchlorosilane; N-Methyl-N-tert-butyldimethylsilyltrifluoroacetamide; MTBSTFA + 1% TBDMSCl; Silylating mixture VI

Silica gel 110 NH2

Activated magnesium silicate; Magnesium silicate

Silica gel

TMSH

Amberlite(R) IR120 hydrogen form

Methanolic HCl

N-Methyl-N-(trimethylsilyl)trifluoroacetamide; N-Trimethylsilyl-N-methyl trifluoroacetamide; MSTFA

Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous

Silylating mixture V; BSTFA + TMCS

System

Sepax C-Pro|Sefia|Sepax 2

Sodium Na

50 ppb max.

Solubility Information

Slightly soluble in sodium hydroxide. Insoluble in water,ethanol,ether and dilute mineral acids.

Solubility aq. soln.: insoluble.

Soluble in hydrofluoric acid. Insoluble in water.

Soluble in water (333.33mg/ml).

Stability

Stable

Sterilization

0.1 Âµm sterile filtered

Sulfate

0.001% max.

0.005% max.

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Theoretical Plates

> 13000 m-1

5000 m-1

> 48 000 m^-1

> 42 000 m^-1

Stokes Radius

~ 10

TSCA

Yes

Surface Area Metric

˜35 m2/g

Vapor Density

>1 (vs air)

<1 (vs air)

Vapor Pressure

8.8 mmHg ( 27 °C)

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

Viscosity

1.98 mPa/s at 25Â°C

Water

0.04% max.

2% max.

1% max.

Target Organs

Respiratory system

Type

Silica

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Well Volume

8.5

WGK

nwg

Width Metric

4 cm

Titratable Acid

0.001mEq/g max.

Viscosity Max

Wetted Part

Width English

1.57 in.

Zinc Zn

20 ppb max.

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