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Pacific Biosciences of California

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Animal Derivative

None

Binding Capacity

12 µg Based on specified 15 µL aliquot of supplied magnetic bead suspension = 1.5 mg beads @ 100 mg/mL

CAS_NO

74299-50-6

Certificate of Analysis

Yes

Animal Origin

Bovine, Porcine

Chemical Substance ID

24894029

Concentration

10 mM

100 mM

25 mM

EC Number

277-809-2

Functional Testing

Each batch of PuReTaq Ready-To-Go PCR Beads is tested in a real-time PCR assay to ensure its ability to generate a specific PCR product with Î» DNA; and with negative control samples (untemplated reactions) to ensure the lowest possible levels of either e

Hazardous

Brand

Life Sciences

Labelor Dye

Unconjugated

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Kit Contents

Room-temperature stable beads containing ~ 2.5 units of recombinant PuReTaq DNA polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction buffer. When a bead is reconstituted to a 25 Î¼L final volume, the concentration of each dNTP is 200 Î¼M in10

Bulk first-strand cDNA reaction mixes (5)DTT solutionpd(N)₆ primerNot I-d(T)18 bifunctional primermRNA standardRNase-free water

Ready-To-Go GenomiPhi V3 cakes, 1 Ã— 96-well plate; 2x Denaturation buffer, 1 Ã— 1.2 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps

MDL Number

MFCD00080335

Label Type

Unlabeled

Molecular Weight

535.15

0.0

Number of Tests

55 reactions

Pack size

55 reactions

96 reactions

Order Information

Custom options of the product, including larger pack sizes, are available. Please contact your local sales representative or Technical Support.

Physical State

Solid

Product Size Avg

> 10 kb

Quantity

0.2mL (Total: 8-µmol)

4 x 0.25mL (4 x 25 -µmol of 100mM solution)

1mL

4 x 1mL (4 x 100 -µmol of 100mM solution)

1mL (Total: 40-µmol)

Quantity

5 x 1mL

5 x 1mL (Total: 200Âµmol)

Size

1,000µl

200µmol

40µmol each

10µmol each

Select Product

Multiwell Plate, 5 x 96 Reactions

0.2 ml hinged tube with cap, 96 Reactions

Sample Weight

10 ng of genomic DNA; or non-purified cell lysates

Storage Temperature

Freezer: - 20 C

Room temperature

Reaction Time

1.5-2 h

Refrigerated/Frozen

Frozen

Shelf Life

120 Days

Storage Conditions

-15 to -30Â°C

Ambient temperature in pouch with desiccant

Store the kit at ambient temperature. Once opened, completely reseal the pouch containing the Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended. Store recons

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