First-Strand cDNA Synthesis Kit, 55 reactions
For synthesis of singlestranded cDNA prior to amplification by PCR.
Features preassembled bulk reaction mixes to save time and minimize potential for pipetting errors.
Allows users to choose primers.
Includes a choice of two primers: pd(N)6 random hexamers and NotI(dT)18.
FirstStrand cDNA Synthesis Kit is designed to generate fulllength firststrand cDNA from mRNA templates. Following firststrand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for secondstrand synthesis using the GublerHoffman method. Primers are not included in the preassembled FirstStrand Reaction Mixes, thus allowing use of a primer of choice. Two primers are included with the kit. pd(N)6 Primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5'ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail.
The NotI(dT)18 Bifunctional Primer can be used to prime selectively on mRNA that has a poly(A) tail. It can also be used as a PCR primer following firststrand synthesis. The NotI restriction site, located at the 5'end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products.
FirstStrand cDNA Synthesis Kit contains the following reagents, sufficient for up to 55 firststrand syntheses, each of which will produce enough material for up to 15 amplification reactions: Bulk FirstStrand cDNA Reaction Mixes, mRNA Standard, DTT Solution, RNaseFree Water, pd(N)6 Primer, NotI(dT)18 Bifunctional Primer, and instruction booklet.