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Absorbance

0.02 AU max. at 330nm

Temperature

45°C

60°C

80°C

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Additives

Without: Calcium, magnesium, phenol red

Without: Calcium, magnesium

Application

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Assay Percent Range

99.8% min by GC

Application_4

Cell isolation

Chromatography system|Manual

Average sample loading capacity

50 mg

400 mg

Bed Dimensions

16 x 25 mm

26 x 600 mm

7 x 25 mm

16 x 100 mm

16 x 600 mm

1.6 x 2.5 cm

10 x 100 mm

26 mm x 400 mm

6.4 x 100 mm

Applications

HPLC: suitable

LPLC: suitable

gas chromatography (GC): suitable

solid phase extraction (SPE): suitable

Bed Volume

120

320

5 mL

1 mL

Bed Height

600 mm

100 mm

25 mm

Base Style

Flat Bottom

Insert Vial

Bed Support

Filter

Net

Assay

=99%

Autoignition Temp

800 °F

~800 °F

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

up to 15mg histidine-tagged protein

125 mg human IgG/column

At least 10 mg histidine-tagged protein/ml sedimented medium.

Bed Height With Two Adaptors

0 to 200 mm

58 mm - 106 mm

Capacity

=25 % absorption capacity (water)

0.8 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

200 µL

1.1 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

4.7 meq/g by dry weight

1.8 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

1.7 meq/mL by wetted bed volume

Binding Capacity/ml Chromatography Medium

At least 10 mg histidine-tagged protein

Up to 20 mg histidine-tagged protein

CarbonLoad

11%

20%

18%

15%

Boiling Point

2230 C

189°C

Brand

Axygen®

BioProcess resin

yes

Bore Size (mm)

Buffer

Running Buffers

Bed Height With One Adaptor

0 mm-29 mm

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

CAS_NO

112926-00-8

1343-88-0

69011-20-7

104219-63-8

39389-20-3

9003-70-7

7631-86-9

9052-95-3

CAS Registry Number 1

7631-86-9

67-68-5

ChEBI

CHEBI:28262

Chem AlertStorageSymbol

Gray

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chemical Stability

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Commonly used aqueous buffers

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Chemical Name or Material

Silica Gel

Dimethylsulfoxide

Chemical Substance ID

24852837

24870506

24857358

329759181

329763462

329750331

329763461

329759187

24852304

24854187

329756032

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Chromatography technique

Ion Exchange Chromatography

Hydrophobic Interaction Chromatography

Affinity coupling

Histidine-tagged protein purification

Antibody affinity chromatography

GST-tagged protein purification

Group specific affinity

Chemical/Physical Resistance

Temp. Limits:. -10 C to 180 C isothermal and programmed

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

ClosureType

Screw

Snap Cap

Screw Cap

Screw Top

Crimp

Closure Color

Black

Silver

Closure Type

With Titeseal Open-bottom Plug Style Plastic Closures, Unattached

Crimp Cap

Screw cap

Screw

ClosureMaterial

Polypropylene

Closure Material

Aluminum

Plastic

Polyethylene

Polypropylene

Closure Size

11 mm

13 mm

20 mm

9 mm

Column Type

capillary chiral

capillary extremely polar

capillary polar

Reversed Phase

capillary non-polar

packed GC

Mixed Mode

Column Format

Analytical Column

Analytical Column, EASY-Spray

Color

Colorless to Slightly Yellow

Natural

White

N/A

Color

Grey

Yellow

Gold/Brown

Amber

Black

Clear

Red

Gold

Silver

Colorless

Brass

Column i.d.

26 mm

10 mm

16 mm

50 mm

5 mm

Composition

Discovery ® DSC-18, 400 mg

bed wt., 500 mg

magnesium sulfate, 150 mg

Tris Base, Sodium Chloride

Column Capacity

< 1 mg/mL

Column volume

1 mL

8 mL

Compatibility

Compatible with most QuEChERS shakers.

Concentration

20 X

Density Max.

1.2 g/ml

1.078 g/ml

ClosureSize

11 mm

9 mm

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Density

1.100g/cmÂ³

Decomposition Information

>190°C

Diameter Metric

75 µm

2.1 mm

Diameter Metric Inner

12 mm

6.6 mm

0.53 mm

15 mm

Diameter MetricOpening

9 mm

11 mm

0.12 µm

0.20 µm

Diameter Metric Opening

11 mm

Diameter Metric Outer

12 mm

15 mm

8 mm

32 mm

Diameter Metric

4.6 mm

EC Number

215-681-1

238-878-4

215-609-9

200-838-9

231-153-3

231-545-4

262-373-8

Exclusion Limit Peptides

4 x 10Â³

Endcapped

Proprietary

Endcapped

Yes

Feature

closure type black phenolic solid top cap

without needle Luer Tip

closure type screw top vial

septum type Thermogreen ® LB-2

Endcapped

syringe type Sample Lock

preconditioned

Not Endcapped

luer lock without slots

needle type N (cemented)

temperature limit 250 °C

Flow Velocity

< 150 cm/h

â‰¤ 600 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 300 cm/h

< 450 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

<1800 cm/h

15 cm/h|15 cm/h

< 600 cm/h

‰¤ 600 cm/h|‰¤ 600 cm/h

Flow Rate

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

>10 ml/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 20 ml/min|< 20 ml/min

1 mL/min - 4 mL/min

< 20 mL/min

< DarcyÂ´s Law

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

1.6 mL/min

< 2.6 ml/min

1.2 - 2.3

2 ml/min - 10 ml/min

Empirical Formula

MgO3Si

Estimated Shelf Life from Manufacture Date

5 years

Endotoxin Activity Max.

0.12 EU/mg

Flash Point

87°C (188.6°F)

FilmThickness

1 µm

For Use With

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

Flow Direction

Supported bidirectionally. Certain applications may require orientation, i.e., vents. Reverse flow only for low pressure applications.

For Use With

ELSD, UV Detector

HPLC

Form

Liquid

fine crystals

powder or crystals

powder

fine powder

Format_6

Prepacked column

Kits

Prepacked columns

Bulk resin

Formula

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

MgO₃Si

Service & Support

O2Si

Exclusion Limit Small Organics

5 x 10Â³

Format

matrix material

Field14

50Â°-65Â°

Format

Liquid

Formula Weight

100.39

78.13

60.08

Application_5

Chromatography system|Manual

H-Codes

H371

H317

H319

H315-H319-H335

H351

H372

Grade

HPLC

Standard

high-purity grade

certified reference material

high-purity grade (Davisil Grade 923)

high-purity grade (7754)

Health Hazard 2

WARNING!

Height Metric

45 mm

32 mm

Height English

1.77 in.

Identification

Pass Test

Grade

Technical Grade

Graduations

0.5 mL

Hold-up Volume of Membrane

<20 Î¼l

<10 Î¼l

<5 Î¼l

COLOR

Blue

Dimensions

H x W x D: 660 x 860 x 710

Filtrate Vessel Material

Polycarbonate (PC)

Finish

Plain top

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Includes

Connectors for high- and low-pressure chromatography equipment and instructions.

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Instructions supplied with the concentractors

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Ionic Capacity

0.29-0.35 mmol Cl^-/ml medium

0.18-0.25 mmol H⁺/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.15-0.20 mmol H⁺/mlÂ

40-85 Âµmol/ml (Cl^-)

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

Inlet Connector

Stepped barb for 10 to 12 mm (3/8 to 1/2") tubing

Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing

Inlet id

0.75 mm (0.029 in)

1 mm (0.04 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

Insert Type

350µL Fused Insert

Fused Insert

250µL Fused Insert

Pre-Slit

IOD-Codes

Includes

Two (0.5 m and 2 m L) 1/16 in. OD PTFE tubing assembly with 1/4-28 UNF PP fitting at one end, a frit kit (contains two each of 10 µm PE, 10 µm PTFE, and 30 µm PTFE frits), and a Click-N-Seal 10-32 PEEK conical port fitting.

Fritted disc, 2mm bore PTFE stopcock plug and 24/40 standard taper upper joint

100 vials and 100 seals with septa

Vials and Closures

3.5 in. Coil

Length Metric

250 mm

150 mm

100 mm

500 mm

50 mm

Ligand

Sulphopropyl

Alkaline-stabilized protein A-derived (E.coli).

Octylamine

Carboxymethyl

Calmodulin

Strep-Tactin XT

Alkali-stabilized Protein A-derived (E. coli)

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Native protein A

TALONÂ® Superflow Cobalt-IMAC

Diethylaminoethyl

Cibacron Blue

Human IgG

Quaternary amine

Porcine heparin

Recombinant protein A (E.coli)

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Ligand Concentration

4.5-8 mg gelatin/ml drained resin

~25 Âµmol phenyl/mL resin

~45 Âµmol phenyl/mL resin

~4mg heparin/mL resin

Ligand Coupling Method

Epoxy

Maleimide activation

Ether linkages and hydrophilic spacer arm

Cyanogen bromide activation

Triazine

Ether linkages

Epoxy activation

Reductive Amination

Ligand_7

2-mercaptopyridine

Protein L

Protein G

Butyl, octyl, phenyl

Protein A, alkali stablilized

Protein A

Protein A, alkali stabilized

Ion Exchanger Type

Weak anion exchanger

Weak and strong anion

Strong anion exchanger

Weak anion

Weak cation exchanger

Strong cation exchanger

Linear Formula

SiO2

MgO3Si

(CH₃)₂SO

(C10H12 · C10H10 · C8H8 · C3H9N)X

(C10H12 · C10H10 · C8H8)x

Material

clear glass vial

Glutathione Sepharose 4 B

Mixed

PTFE body

PTFE/silicone septum

polypropylene body

red PTFE/silicone septum

Stainless Steel Hardware (Column)

Tedlar ® PVDF film

white PTFE/silicone

white rubber liner

Clear Glass

Plastic/Stainless Steel

PTFE

silicone body

stainless steel TD tube (includes screens)

bevel tip stainless steel needle (point style 2)

Cellulose

polypropylene material (hardware)

PTFE-faced Rubber Liner

PTFE/silicone septa

Silica Gel

Stainless Steel

Stainless Steel Hardware

VG2, 60% Vespel/40% Graphite

Borosilicate Glass

Polypropylene

Polypropylene (PP)

PTFE/red rubber septum

stainless steel TD tube

Brass

Amber Glass (Type 51A)

PEEK

Phenolic

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

polypropylene tube

Glass

Glass Body

PE frit (20 µm porosity)

PE frit (20/40 µm)

PTFE Luer lock

high-density polyethylene vial

Metal

PTFE/silicone

Rubber

stainless steel column

stainless steel holder

(Multi-Layer Foil: (1) 0.002" Polyethylene (2) 0.0003" Aluminum Foil (3) Polyethylene (4) Aluminum (metalized) (5) 60 Gauge Nylon)

black polypropylene cap

clear glass insert

glass TD tube

PE frit

Polycarbonate (PC)

SilTite

stainless steel frit

Acetal plastic (POM)

blue polypropylene cap

cone tip, side port stainless steel needle (point style 5)

N/A

polypropylene hardware

Material Septa

PTFE/Silicone/PTFE

PTFE/Silicone

Matrix

Composite of cross-linked agarose and dextran

Highly cross-linked 6% agarose

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

agarose, 6%, spherical

Composite of cross-linked agarose

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Cross-linked agarose, 4%

cross-linked agarose, 4%, spherical

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

cross-linked, 6% agarose particles

Highly cross-linked agarose

2% agarose

Cross-linked dextran

Rigid, cross-linked agarose, spherical

Matrix

Highly cross-linked 4% agarose

6% Cross-Linked Agarose

Highly cross-linked agarose with dextran surface extender

4% Agarose

4% Cross-Linked Agarose

Highly cross-linked agarose, spherical.

Max Pressure

11600 psi (800 bar)

4000 psi (275 bar)

900 psi

14500 psi (1000 bar)

5800 psi (400 bar)

4500 psi (310 bar)

17400 psi (1200 bar)

Ligand Density

~ 5 mg/mL resin

3 mg GammaBind G, type 3/ml medium

Approx. 13 Î¼mol/ml

3 mg Protein A/ml medium

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

6 mg rProtein A/ml medium

2 mg Protein G/ml medium

MDL Number

MFCD00132712

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00132722

MFCD00132707

MFCD00132771

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00145586

MFCD00011232

MFCD00084428

MFCD03791384

MFCD00146442

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00149897

MFCD00211881

MFCD00145841

MFCD00132726

MFCD00145831

Kit Contents

Aprotinin (10 mg): 6500 M_r
Ribonuclease A (50 mg): 13 700 M_r
Carbonic anhydrase (15 mg): 29 000 M_r
Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Blue Dextran 2000

BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL

RNAspin Lysis Solution (Red sticker): 25 ml, RNAspin Wash Buffer I (Yellow sticker): 15 ml, RNAspin Wash Buffer II (Grey sticker): 12.5 ml Add 50 ml Ethanol; RNAspin Desalting Buffer (Blue sticker); 25 ml, RNAspin Desalting Reaction Buffer (Green sticker)

RNAspin Lysis Solution (Red sticker): 125 ml, RNAspin Wash Buffer I (Yellow sticker): 80 ml, RNAspin Wash Buffer II (Grey sticker): 3 Ã— 25 ml Add 100 ml Ethanol to each bottle; RNAspin Desalting Buffer (Blue sticker); 125 ml, RNAspin Desalting Reaction B

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Aldolase (50 mg): 158 000 M_r
Ferritin (15 mg): 440 000 M_r
Thyroglobulin (50 mg): 669 000 M_r
Blue Dextran 2000 (50 mg

MarkingSpot

Yes

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Liner Material

PTFE/Silicone

None

Polypropylene

PTFE/Rubber

Marking Spot

Max Temperature

330/350°C for Standard Columns; 430°C for Metal Columns

340/360°C

40°C

Licensing

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand

Recombinant GammaBind G type 3 lacking albumin-binding region

Ion exchanger

S (cation exchanger)

Q (anion exchanger)

Material

100% Dimethyl Polysiloxane

Fused Silica

Borosilicate Glass, PTFE, Polyethylene, FKM/FPM

PTFE Frit

StableFlex Fiber

Intrinsic Viscosity

~ 17

Length Metric

60 m

750 mm

30 m

400 mm

457 mm

20 mm

Molecular Weight

0.0

60.08

178.13 (anhydrous basis)

100.39

78.129

Material Coating

Polypropylene

IUPAC Name

methylsulfinylmethane

Max Temperature

445°C

Length English

18 in.

Ligand Type

Monomeric C18

No per Pack

100

Pack size

10 mL

200 mL

25 mL

500 g

6 x 500 mL

50 mL

1000 mL

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 Ã— 100 ÂµL

50 columns

6 mL

7 Ã— 1 mL

1 L

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

100 mL

100 g

5 mL

500 mL

1 piece

20 mL

300 mL

1 Ã— 4.7 mL

2 x 1 mL

5 Ã— 1 mL

Packaging Layout

100, 500 g in poly bottle

2.5, 5, 25 kg in fiber drum

1, 6x1, 2.5 kg in poly bottle

1 kg in poly bottle

1 ft3 in fiber drum

25, 500 g in poly bottle

Particle Shape

Spherical

Particle Size in Methanol Min

Particle size, d_50V

~85 Âµm

~90 Âµm

34 Âµm

~34 Âµm

~34 Î¼m

~ 9 Âµm

~90 Î¼m

~75 Âµm

~ 8.6 Âµm

ParticleSize

5 µm

3 µm

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

pH stability, CIP

1â€“14

2â€“14

2â€“9

2â€“10

3â€“14

2â€“13

2â€“13.7

3â€“12

2â€“14Co²⁺-stripped medium

3â€“10

4â€“13

Pore Size

60 +?

60 Å mean pore size

70 Å pore size

110 Å pore diameter

60 Å pore size

0.75 cm 3 /g pore volume

180 Å

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

100 Å

0.7-0.9 cm 3 /g pore volume

30 Å

300 Å

90 Å pore size

160 Å

0.7-0.85 cm 3 /g pore volume (approx.)

100 Å

150 Å

90 Å mean pore size

~0.55 mL/g pore volume

120 Å

90 Å

1000 Å

Porosity

20 Î¼m

7 Î¼m

10 Î¼m

10 or 80 Âµm

Melting Point

18.4°C

Pressure/Flow Specification

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

Molecular Formula

C2H6OS

O2Si

Optical Activity

~7 (5% in H 2 O, suspension)

6.5 (5% in slurry)

7.0 (5% in slurry)

Packing Material

Spherical, Fully Porous, Ultrapure Silica

Spherical, Nanopolymer Hybrid Silica

Partical Size Dry

18 Âµm-111 Âµm

40 Âµm-120 Âµm

50 Âµm-150 Âµm

pH stability, operational

2â€“10

pH 6 to 10

2â€“9

4â€“13

4â€“9

3â€“10

2â€“12

2â€“13

4â€“12

3â€“12

3â€“9

3 to 12

3â€“13

Physical State

liquid

liquidSuspension

solid

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Operating Flow Rate, Recommended

1.3 mL/min

0.075 mL/min

0.80 mL/min

0.5 mL/min

Operating pH

1 - 9

2 - 9

3.8 - 6.8

2 - 7.5

0 - 14

2 - 8

Particle Size

24 Âµm-44 Âµm

60 Âµm-165 Âµm

45 Âµm-165 Âµm

45 Î¼m-165 Î¼m

24-44 Âµm

2 µm

60 Î¼m-160 Î¼m

pH Stability Cleaning-in-Place (CIP)

1 to 14

Pictogram

GHS08

GHS07

PoreSize

300 Å

p H

4 to 13

7.5 pH

2.5 to 7

2 to 8

Pack Size

Packaging

Amber Glass

No per Pack

100

Percent Purity

≥99.9%

Operating Flow Rate, Max

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

1.0 mL/min

2.6 mL/min

7.0 to 7.4

pH Range

5.5â€“7.5

8.9â€“9.9

8.5â€“9.5

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Liquid

Order Information

For research use only.

Percoll PLUS is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

HiLoad columns are prepacked XK columns.

Osmolality

< 25 mOsm/kg

< 30 mOsm/kg

pH ligand fully charged

Above 6

Entire pH range

Below 9

Purity

>=99%

Quantity

1000

144 Pack

200

Quantity

1000/Cs.

100

1000/Pk.

100 g

100 Pack

100/Pk.

500 mL

Preslit

Yes

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Phase

Reversed Phase and Ion Exchange

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Refractive Index

1.4770 to 1.4800 (at 25°C)

>1600 C

Outlet id

0.75 mm (0.029 in)

1.0 mm (0.04 in)

1.55 mm (0.06 in)

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Height English

1.25 in.

Housing

Polypropylene (PP)

Osmolality

288-310 mOsm/kg

Product Line

Easy Spray

Pierce

Sample Volume

< 0.5 mL

< 5 mL

600 Âµl

<= 15 ml

<= 5 ml

<= 13 ml

4 to 50 ÂµL

<= 1.5 ml

25 to 500 ÂµL

S-Codes

26-36

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

P-CODES

P280

P314

P305 + P351 + P338

Particle Size in Methanol Max

163

Noper Pack

100

Phase

Reversed Phase

Signal Word

Warning

Danger

Preslit

Pressure Limit

20 bar (2 MPa, 290 psi)

Recommended maximum operational flow velocity

300 cm/h

Residue After Evaporation

5ppm max.

Resin Type

Capto

Sepharose Fast Flow

MabSelect

Sepharose High Performance

RIDADR

NONH for all modes of transport

Quality

monodispersed

with moisture indicator free of heavy metals

dry

SMILES

CS(=O)C

R-CODES

36/37/38

48/20

PurityGrade Notes

Mobile phase for HPLC applications

Storage

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

2Â°Câ€“8Â°C, 20% ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2-8C

4Â°C to 8Â°C

2 to 8Â°C, 20% Ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

Sterilization

May be autoclaved at 121Â°C for 20 minutes. Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed after autoclavin

0.1 Âµm sterile filtered

Scale

Lab

Lab|Process development|Process

Lab|Process development

Lab|Process

Pub Chem CID

679

Septum

Red PTFE/White Silicone

Sealing

Heat-fused

Surface Area

~500 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

300 m 2 /g

470-530 m 2 /g

22 m 2 /g

320 m 2 /g

450 m 2 /g

170 m 2 /g

=480 m 2 /g (approx.)

200 m 2 /g

430-530 m 2 /g

475 m 2 /g

80 m 2 /g

450 m²/g

100 m 2 /g

500 m 2 /g

100 m²/g

250-350 m 2 /g

530 m 2 /g

300 m²/g

289 m 2 /g

450 m 2 /g

480 m 2 /g

Septum

None

Purity GradeNotes

Mobile phase for HPLC applications

Residueafter Evaporation

5ppm max.

Swelling factor

~1.7

Recommended Flow Rate

1 ml/min

Shelf Life

24 months

Storage Conditions

4ËšC to 30ËšC, 20% ethanol

4 - 30Â°C

4â€“30Â°C

At room temperature. Precipitation can occur at low temperature.

Temperature

60°C

Solubility Information

Solubility aq. soln.: insoluble.

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Stationary Phase

Reversed Phase/Anion Exchange/Cation Exchange

C18

Stability

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stokes Radius

~ 10

Support System

Polypropylene (PP)

Synonyms

QuEChERS; QuEChERS

Tedlar(R) Gas Sampling Bag

XAD16N

Activated magnesium silicate; Magnesium silicate

QuEChERS

Silica gel

Suitability

Autosampler compatibility:Agilent Agilent 1050/1090A Agilent 1100/1200 Agilent 7673A, 7683 Agilent 5890/7985A/6890Hitachi AS-1000, AS-2000, AS-4000 L7200/L7250LEAP Technologies CTC LC PAL, GC PAL CombiPAL HTC PAL/HTS PALPerkinElmer Autosystem/AS-2000/XL

Type

HPLC Column

Liquid Chromatography (LC)

Analytical Column

Vapor Density

<1 (vs air)

Viscosity Max

Volume

100-500 Âµl

1 mL

5-20 ml

2 mL

25 mL

0.5 mL

2-6 ml

400 Âµl - 2 ml

12 ml

22 mL

3 mL

50 mL

6 mL

Volume Metric Usable

1.5 mL

Synonym

DMSO

Wetted Part

yes

Total Time of Preparation

< 30 min/6 preps

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Vent

On inlet

Volume MetricTotal

2 mL

U SPType

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

Theoretical Plates

5000 m-1

> 48 000 m^-1

> 38 000 m^-1

> 13000 m-1

> 43 000 m^-1

Volume_28

400 Âµl - 2 ml

2-6 ml

100-500 Âµl

5-20 ml

Operating Pressure Max

4.1 bar (60 psi)

Thread Size

10-425

20 mm

9 mm

WGK

nwg

Volume Metric

2 mL

Volume Metric

2.0 mL

4 mL

Water

0.04% max.

Volume Metric Working

1.8 mL

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Volume Metric Residual

<170µL

Volume MetricUsable

1.5 mL

4 mL

Volume MetricResidual

<170µL

Volume English

1 dr.

ProductLine

Acclaim

Total binding capacity

~50 mg hIgG/mL resin

VialType

11 mm Crimp Top Vial

9 mm Target DP Vial

RTECS Number

VV7315000

USP Type

Viscosity

1.98 mPa/s at 25Â°C

Volume Metric Total

2 mL

System

Sepax C-Pro|Sefia|Sepax 2

Titratable Acid

0.001mEq/g max.

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