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Application_4
Cell isolation
Chromatography system
Chromatography system|Manual
Assay
=99%
99.8%
Boiling Point
2230 C
Brand
Restek
Axygen®
Capacity
=25 % absorption capacity (water)
1.1 meq/mL by wetted bed volume
1.35 meq/mL by wetted bed volume
3.0 meq/g by dry weight
0.8 meq/mL by wetted bed volume
1.3 meq/mL by wetted bed volume
0.6 meq/mL by wetted bed volume
200 µL
1.7 meq/mL by wetted bed volume
1.8 meq/mL by wetted bed volume
4.7 meq/g by dry weight
0.7 meq/mL by wetted bed volume
1.0 meq/mL by wetted bed volume
1.2 meq/mL by wetted bed volume
Applications
HPLC: suitable
gas chromatography (GC): suitable
solid phase extraction (SPE): suitable
ion chromatography: suitable
LPLC: suitable
Application_5
Chromatography system|Manual
Bead type
Mag Sepharose Magnetic Beads
Autoignition Temp
~800 °F
800 °F
Average sample loading capacity
400 mg
50 mg
CAS_NO
104219-63-8
11128-95-3
126850-14-4
112926-00-8
1343-88-0
112945-52-5
1344-28-1
7631-86-9
39389-20-3
9003-70-7
69011-19-4
9052-95-3
69011-20-7
Chemical Stability (Column Hardware)
Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.
Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons
Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons
Chemical Substance ID
24852837
24857358
24854194
329752001
24865203
24852304
329759188
24870506
329752189
329759193
329759181
24854823
329759183
329756032
329759187
329768201
329768868
329750331
329763462
329763461
Composition
bed wt., 3 g
bed wt., 500 mg
bed A, 1.25 g (Supelclean ™ LC-Florisil ® )
bed wt., 2 g
bed wt., 50 mg/well
magnesium sulfate, 150 mg
nitrogen content, =1.0%
bed wt., 350 mg
magnesium sulfate, 6 g
sodium bicarbonate, 5 g (Cat. No. S6014)
bed wt., 1 g
Discovery ® DSC-18, 400 mg
sodium chloride, 1 g (Cat. No. S7653)
Z-Sep+, 500 mg
bed wt., 50 mg
bed wt., 100 mg/well
bed wt., 100 mg
Supelclean ™ ENVI-Carb, 45 mg (Cat. No. 57210-U)
bed B, 2 g (Supelclean LC-Si)
bed wt., 30 mg
Column volume
8 mL
1 mL
Chromatography technique
Antibody affinity chromatography
Group specific affinity
GST-tagged protein purification
Hydrophobic Interaction Chromatography
Histidine-tagged protein purification
Ion Exchange Chromatography
Affinity coupling
Antibody affinity chromatography.
Multimodal ion exchange chromatography
Chemical/Physical Resistance
Temp. Limits:. -10 C to 180 C isothermal and programmed
Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.
Column Type
packed GC
capillary chiral
capillary intermediate polar
capillary non-polar
capillary extremely polar
capillary polar
Column Capacity
< 1 mg/mL
Df
0.12 µm
85 µm
0.20 µm
0.25 µm
5.00 µm
Compatibility
Compatible with most QuEChERS shakers.
Color
Clear
Amber
White
Diameter
47 mm
12 mm
1/2 in.
EC Number
262-373-8
231-153-3
215-681-1
231-717-9
215-609-9
231-545-4
215-710-8
200-838-9
238-878-4
Concentration
1 N in NaOH (Regenerant concentration)
Feature
swivel design
for cap size 15 mm
glass stopcock plug
KEL-F hub
closure type black phenolic hole cap
3.6 mm orbit
Endcapped
without needle Luer tip
closure type screw top vial
biocompatible
autoclavable
unconditioned
KH (Knurled Hub)
closure type black phenolic solid top cap
septum replaceable Thermogreen LB-2
temp. limit 135 °C
(septa bonded to cap)
vial type headspace
needle type N (cemented)
needle type RN (removable needle)
temperature limit 250 °C
luer lock without slots
precleaned
syringe type Sample Lock
temp. limit 250 °C
screw caps attached
Not Endcapped
septum type Thermogreen ® LB-2
preconditioned
Flow Rate
> 20 ml/min
1 mL/min
< 20 ml/min
< 31 ml/min
1.6 mL/min
5 ml/min - 20 ml/min
0.5 ml/min - 3.0 ml/min
4 ml/min (1 ml), 5 ml/min (5ml)
0.8â€“3.9 mL/min
< 15 ml/min
< 1 ml/min
< 5 ml/min
< DarcyÂ´s Law
Recommended: 0.5 to 2.0 mL/min
< 4 ml/min
0.8-3.9 ml/min
< 10 ml/min
> 4 ml/min
< 2.6 ml/min
2 ml/min - 10 ml/min
5 mL/min
1.2 - 2.3
< 1.0 ml/min
>10 ml/min
>60 ml/min
1-4ml/min
1 ml/min - 4 ml/min
Flow Velocity
< 130 cm/h
< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height
<1800 cm/h
150 cm/h - 600 cm/h
30-150cm/h
<15 cm/h
15 cm/h
< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height
< 300 cm/h
< 450 cm/h
< 500 cm/h
< 50 cm/h
100 cm/h - 300 cm/h 1
< 300 cm/h, using XK 50/30 column with 15 cm bed height
< 60 cm/hour
< 600 cm/h
10 cm/h - 50 cm/h
< 150 cm/h
Up to 40 cm/h
â‰¤ 600 cm/h
Field14
50Â°-65Â°
Format
matrix material
Empirical Formula
O2Si
MgO3Si
Form
powder
powder or crystals
beads
fine powder
fine crystals
Flashpoint (°C)
> 240 C
Density
1.01 g/mL at 25 °C (true wet)(lit.)
For Use With
For all Biacore SPR systems.
For use in all Biacore SPR systems
HiTrap and HiPrep columns (connecting column to AKTAdesign systems).
Connect a syringe to the top of a HiTrap columns.
The volumes are designed for Biacore 8K+, Biacore 8K but the kit can be used with all Biacore SPR systems
Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100
Format_6
Prepacked column
Prepacked columns
Bulk resin
Kits
Exclusion Limit Peptides
4 x 10Â³
Formula
[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄
(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x
Service & Support
ZrOCl₂·xH₂O
MgO₃Si
O2Si
(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃
Exclusion Limit Small Organics
5 x 10Â³
Filtrate Vessel Material
Polycarbonate (PC)
Formula Weight
100.39
For use with_31
AKTA go protein purification system
Grade
high-purity grade (Davisil Grade 643)
high-purity grade (Davisil Grade 646)
high-purity grade (Davisil Grade 923)
high-purity grade (Davisil Grade 636)
reagent grade
certified reference material
high-purity grade (Davisil Grade 62)
high-purity grade (7754)
high-purity grade (Davisil Grade 633)
nuclear grade
standard
for column chromatography
High-purity Grade
technical grade
H-Codes
H315-H319-H335
H373
H351
H371
H317
H319
H372
Group To be Coupled
-NHâ‚‚
Primary amines
Guidance
For AKTAprime
Hold-up Volume of Membrane
<20 Î¼l
<10 Î¼l
<5 Î¼l
HAZ
No
items per unit of measure
400 covers
100 caps
125 mL
1 each
3 columns
5 columns
50 g
2 columns
400 sample pans
100 inserts
Ion Exchanger Type
Weak cation exchanger
Strong anion exchanger
Weak anion
Multimodal weak cation exchanger
Strong cation exchanger
Weak anion exchanger
Weak and strong anion
Ionic Capacity
0.11â€“0.16 mmol (Cl^-)/mL resin
0.14-0.20 mmol Cl^-/ml
0.16-0.22 mmol Cl^-/ml medium
0.09-0.13 mmol H⁺/ml medium
0.13-0.17 mmol/ml
0.15-0.20 mmol H⁺/mlÂ
0.18-0.25 mmol H⁺/ml medium
3-4 mmol/g
0.18-0.24 mmol Cl^-/ml medium
0.10â€“0.14 mmol Cl^- / mL resin
0.29-0.35 mmol Cl^-/ml medium
40-85 Âµmol/ml (Cl^-)
Includes
1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns
Instructions supplied with the concentractors
Connectors for high- and low-pressure chromatography equipment and instructions.
Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.
2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)
Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''
Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.
Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.
Inlet id
1 mm (0.04 in)
1.0 mm (0.04 in)
2 mm (0.08 in)
0.75 mm (0.029 in)
1.55 mm (0.06 in)
Intrinsic Viscosity
~ 17
IOD-Codes
Xi
Xn
T
Ion exchanger
S (cation exchanger)
Q (anion exchanger)
Kit Contents
BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL
RNAspin Lysis Solution (Red sticker): 25 ml, RNAspin Wash Buffer I (Yellow sticker): 15 ml, RNAspin Wash Buffer II (Grey sticker): 12.5 ml Add 50 ml Ethanol; RNAspin Desalting Buffer (Blue sticker); 25 ml, RNAspin Desalting Reaction Buffer (Green sticker)
Aprotinin (10 mg): 6500 M_r
Ribonuclease A (50 mg): 13 700 M_r
Carbonic anhydrase (15 mg): 29 000 M_r
Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Blue Dextran 2000
Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Aldolase (50 mg): 158 000 M_r
Ferritin (15 mg): 440 000 M_r
Thyroglobulin (50 mg): 669 000 M_r
Blue Dextran 2000 (50 mg
Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml
Ligand
Alkali-stabilized Protein A-derived (E. coli)
Cibacron Blue
Glutathione and 10-carbon linker arm
Carboxymethyl
Porcine heparin
Calmodulin
Strep-Tactin XT
Human IgG
Octylamine
Sulphopropyl
Diethylaminoethyl
Native Protein A
Recombinant GammaBind G type 3 lacking albumin-binding region
Bovine gelatin
TALONÂ® Superflow Cobalt-IMAC
Recombinant protein A (E.coli)
Glutathione
Recombinant protein G lacking albumin-binding region, produced in E. coli
Phenyl
Quaternary amine
Ligand Coupling Method
Reductive Amination
Triazine
Epoxy
Maleimide activation
Ether linkages and hydrophilic spacer arm
Cyanogen bromide activation
Epoxy activation
Dextran surface extenders
Ether linkages
Ligand Density
Approx. 13 Î¼mol/ml
~16-23 Âµmol NHS/ml drained medium
6 mg rProtein A/ml medium
4.5-8 mg gelatin/ml drained resin
~ 7 Î¼mol Cibacron Blue/ml medium
2 mg Protein G/ml medium
3 mg GammaBind G, type 3/ml medium
3 mg Protein A/ml medium
~ 5 mg/mL resin
Ligand_7
Butyl, octyl, phenyl
Protein L
Protein A, alkali stablilized
Protein A
Protein A, alkali stabilized
Protein G
2-mercaptopyridine
Ligand Concentration
~25 Âµmol phenyl/mL resin
4.5-8 mg gelatin/ml drained resin
~45 Âµmol phenyl/mL resin
~4mg heparin/mL resin
Linear Formula
(C10H12 · C10H10 · C8H8)x
SiO2
(C10H12 · C10H10 · C8H8 · C3H9N)X
MgO3Si
Inlet Connector
Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing
Male national pipe thread (MNPT)
Material
cone tip, side port stainless steel needle (point style 5)
fused silica
glass
PTFE/silicone septa
PE frit
stainless steel frit
white rubber liner
Polycarbonate (PC)
red PTFE/silicone septum
stainless steel hardware (column)
Polypropylene
PTFE/silicone
silver aluminum seal (pressure release)
Glass Body
PTFE-faced rubber liner
polypropylene hardware
PTFE/red rubber septum
stainless steel column
stainless steel holder
stainless steel TD tube
clear glass insert
PTFE body
white PTFE/silicone
Acetal plastic (POM)
bevel tip stainless steel needle (point style 2)
blue polypropylene cap
PE frit (20/40 µm)
polypropylene material (hardware)
StableFlex fiber
blunt tip stainless steel needle (point style 3)
PEEK
high-density polyethylene vial
PTFE Frit
Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)
stainless steel TD tube (includes screens)
aluminum liner
blunt tip Kel-F needle (point style 3)
clear glass bottle
polypropylene tube
silicone body
(Multi-Layer Foil: (1) 0.002" Polyethylene (2) 0.0003" Aluminum Foil (3) Polyethylene (4) Aluminum (metalized) (5) 60 Gauge Nylon)
black polypropylene cap
clear glass vial
glass TD tube
PTFE/silicone septum
Tedlar ® PVDF film
Cellulose
PE frit (20 µm porosity)
PTFE Luer lock
stainless steel
stainless steel hardware
Polypropylene (PP)
polypropylene material
Matrix
cross-linked, 6% agarose particles
Highly cross-linked 4% agarose
Highly cross-linked agarose
Highly crossed-linked agarose, 6%
Agarose, 4%
6% Cross-Linked Agarose
Composite of cross-linked agarose
Highly cross-linked agarose with dextran surface extender
Rigid, cross-linked agarose, spherical
4% Agarose
4% cross-linked agarose
Highly cross-linked agarose, 6%
agarose, 6%, spherical
2% agarose
Cross-linked agarose, 4%
cross-linked agarose, 6%, spherical
Highly cross-linked agarose, spherical.
Hydroxypropylated, cross-linked dextran
cross-linked agarose, 4%, spherical
Cross-linked agarose, 6%
Highly cross-linked agarose, spherical
Composite of cross-linked agarose and dextran
Highly cross-linked 6% agarose
Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.
Cross-linked dextran
Licensing
For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements
For licensing information, see the Licensing Statements page in the About Us section.
For licensing information, see the Licensing Statements page in the About Us section.
Housing
Polypropylene (PP)
MDL Number
MFCD00132701
MFCD00135579
MFCD00011232
MFCD00132712
MFCD00132770
MFCD00132771
MFCD00211881
MFCD00148280
MFCD00165824
MFCD00132722
MFCD00084428
MFCD00132726
MFCD00132707
MFCD00132718
MFCD00146764
MFCD00145831
MFCD00149897
MFCD00166483
MFCD03096474
MFCD03791384
MFCD00078249
MFCD00145841
MFCD00146442
Maximum pressure over packed bed
4.0 MPa (40 bar, 580 psi)
Metal Ion Capacity
54 to 70 Âµmol Ni²⁺/ml medium
~15 Âµmol Ni²⁺/ml medium
approx. 15 Âµmol Ni²⁺/ml medium
Molecular Weight
60.08
178.13 (anhydrous basis)
0.0
60.08
101.96
100.39
Operating Flow Rate, Max
0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature
0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature
1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature
1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature
1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature
1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature
1.0 mL/min
2.6 mL/min
Operating pH
2 - 8
3.8 - 6.8
2 - 7.5
1 - 8
0 - 14
0 - 9
2 - 9
0 - 7
1 - 9
3.5 - 7.0
Operating Flow Rate, Recommended
0.75 mL/min
0.04 mL/min
0.30 mL/min
0.5 mL/min
0.50 mL/min
1.3 mL/min
0.075 mL/min
0.80 mL/min
Order Information
HiLoad columns are prepacked XK columns.
Not suitable for use with AKTAprime plus system.
This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery
This product is produced based on a customer order. Delivery time may be longer than for standard products.
Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction
Columns included in HiTrap HIC Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.
HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.
HiLoad columns are prepacked XK columns
Allows safe, reliable storage and transport of self-packed Tricorn columns.
Percoll PLUS is for research use only.
Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow. PD- 10 Buffer reservoir has to be ordered separately For smaller s
Percoll is for research use only.
Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions
Use 20-40 mM imidazole in sample and binding buffers for highest purity.
Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.
Not for use with ultrasalt-sensitive automated DNA Sequencers (e.g. ALFexpress II and ABI 377 model.)
For research use only.
The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.
Outlet id
1.55 mm (0.06 in)
1.0 mm (0.04 in)
0.75 mm (0.029 in)
Operating Pressure Max
4.1 bar (60 psi)
Optical Activity
6.5 (5% in slurry)
~7 (5% in H 2 O, suspension)
7.0 (5% in slurry)
Pack size
1 Ã— 4.7 mL
1 Ã— 5 mL
1 mL
150 mL
6 mL
10 mL
200 mL
25 mL
250 mL
4 x 50 mL
50 columns
500 g
20 mL
25 g
250 preps
50 preps
6 x 100 mL
2 x 1 ml 10% slurry
5 mL
50 mL
1 Ã— 1 mL
1 Ã— 120 mL
100 mL
75 mL
1
2 x 1 mL
4 x 53 mL
50 Ã— 100 ÂµL
6 x 500 mL
7 Ã— 1 mL
1 piece
4.7 mL
30 columns
500 mL
53 mL
1 L
1000 mL
15 g
5
10 Ã— 2 mL
5 Ã— 1 mL
1 kit
1.5 g
2
6 x 1 L
8
1 Ã— 320 mL
10 g
5 Ã— 5 mL
100 g
Packaging Layout
1 ft3 in fiber drum
10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles
1, 6x1, 2.5 kg in poly bottle
500 g in poly bottle
25, 500 g in poly bottle
2.5, 5, 25 kg in fiber drum
500 g in poly drum
100, 500 g in poly bottle
5, 25 kg in fiber drum
2.5 kg in poly drum
1 kg in poly bottle
10, 50, 100, 500 g in poly bottle
25 kg in fiber drum
Vials with caps attached are packed in individual cells in trays of 100.
P-CODES
P260 - P264 - P270 - P308 + P311 - P405 - P501
P280
P260
P305 + P351 + P338
P314
pH ligand fully charged
< 9
Entire pH range
Above 6
Below 9
pH Range
5.5â€“7.5
8.5â€“9.5
8.9â€“9.9
pH stability, CIP
2â€“14
2â€“10.5
2â€“13.7
1â€“14
2â€“10
2â€“13
2â€“9
4â€“9
2 (ligand dependent)â€“13 (ligand dependent)
2â€“12
3â€“10
4â€“13
2â€“14Co²⁺-stripped medium
3â€“12
3â€“14
Particle Size
60 Î¼m-200 Î¼m
24-44 Âµm
45 Âµm-165 Î¼m
45 Î¼m-165 Î¼m
60 Âµm
30 Âµm-40 Âµm
25 Âµm-75 Âµm
60 Î¼m-160 Î¼m
24 Âµm-44 Âµm
60 Âµm-165 Âµm
45 Âµm-165 Âµm
Particle size, d_50V
~85 Âµm
~100 Î¼m
~34 Î¼m
~90 Î¼m
34 Âµm
~90 Âµm
~ 9 Âµm
ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm
~50 Âµm
~ 8.6 Âµm
~34 Âµm
~75 Âµm
pH Stability Working Range
3â€“12
Physical Form
Spray-dried powder
Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)
Colloidal solution of silica particles coated with silane
Pore Size
120 Å
60 Å mean pore size
70 Å pore size
1 Âµm
110 Å pore size
~0.8 cm 3 /g pore volume
100 Å
60 Å pore size
0.7-0.85 cm 3 /g pore volume (approx.)
160 Å
0.2 Âµm
30 Å
0.7-0.9 cm 3 /g pore volume
~1.30 mL/g pore volume
0.30 mL/g pore volume
60 Å average pore size (52-73 Å)
~0.65 mL/g pore volume
110 Å pore diameter
5 Âµm
0.45 Âµm
0.75 cm 3 /g pore volume
180 Å
1000 Å
~0.55 mL/g pore volume
0.45
0.8 cm 3 /g pore volume
90 Å
150 Å
300 Å
90 Å mean pore size
90 Å pore size
Porosity
20 Î¼m
10 or 80 Âµm
7 Î¼m
10 Î¼m
Pressure/Flow Specification
0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height
300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height
150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)
500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar
250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
min 90 cm/h, bed height 10 cm, column 5 cm i.d.
>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.
base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.
base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.
300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.
24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d
Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.
>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.
300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height
Partical Size Dry
20 Âµm-50 Âµm
50 Âµm-150 Âµm
18 Âµm-111 Âµm
>20 Âµm
40 Âµm-120 Âµm
>100 Âµm
Particle size distribution, wet
100â€“300 Âµm
20â€“80 Âµm
55â€“165 Âµm
15â€“88 Âµm
90â€“280 Âµm
pH stability, operational
3 to 12
4â€“9
3â€“9
2â€“10
2â€“13
pH 6 to 10
4â€“12
2â€“12
3â€“10
4â€“13
3â€“11
3â€“13
2â€“9
3â€“12
Physical State
solid
liquidSuspension
liquid
Properties
Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.
Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been
Solution
Outlet Connector
Stepped barb for 10 to 12 mm (3/8 to 1/2") tubing
Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing
Osmolality
322-344 mOsm/kg
288-310 mOsm/kg
< 25 mOsm/kg
276-298 mOsm/kg
< 30 mOsm/kg
Purity
99.8%
>=99%
Quantity
1 × 100
36
108
500
5
100
24
50 g
500 g
25
30
50
200
20
10
3
1
100 g
6
2
54
Pressure/flow characteristics
250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).
150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)
Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).
250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)
Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).
Particle size distribution, dry
50 â€“ 150 Âµm
Reactivity and Stability
The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate
Prefilter
No
Glass microfiber double laminated with polyolefin monofilament non-woven
R-CODES
48/20
36
36/37/38
45-36/38
Sample Volume
<= 1.5 ml
<= 13 ml
600 Âµl
0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)
2.5 ml
4 to 50 ÂµL
100 to 300 Î¼l
< 150 Âµl
<= 5 ml
Up to 8.5 mL
0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)
400 to 1000 Î¼l
< 0.5 mL
< 5 mL
25 to 500 ÂµL
<= 15 ml
Sealing
Heat-fused
Operating Temperature
4Â°C-60Â°C
4Â°C-35Â°C
Outer Diameter
12 or 18 mm
Product Family
Axygen® Gel Loading Tips
Particle Size in Methanol Min
27
Resin Type
Sepharose CL-4B
Sepharose High Performance
Capto
Sepharose Fast Flow
Superdex 200 Increase
MabSelect
Storage Conditions
At room temperature. Precipitation can occur at low temperature.
20% ethanol at 4Â°C to 30Â°C
4 - 30Â°C
4 - 8Â°C, 20% Ethanol, 10% medium slurry
4ËšC to 30ËšC, 20% ethanol
4â€“30Â°C
RIDADR
NONH for all modes of transport
Surface Area
550 m 2 /g
800 m 2 /g
=480 m 2 /g (approx.)
~100 m 2 /g
300 m 2 /g
450 m 2 /g
~30 m 2 /g
~300 m 2 /g
250-350 m 2 /g
170 m 2 /g
~500 m 2 /g
200 m 2 /g
22 m 2 /g
470-530 m 2 /g
320 m 2 /g
430-530 m 2 /g
475 m 2 /g
120 m 2 /g
289 m 2 /g
500 m 2 /g
500 m 2 /g (approx.)
380 m 2 /g
450 m 2 /g
100 m 2 /g
480 m 2 /g
530 m 2 /g
80 m 2 /g
RTECS Number
VV7310000
VV7315000
S-Codes
22
53.1-26-45
26
45
26-36
Signal Word
Warning
Danger
Synonyms
CELLULOSE THIMBLE 43 X 123,PK25
Crimp Seal with Red Rubber/PTFE Septum
Preppy(TM) 12 PORT RACK PLATE, 1 & 4ML VIALS
Silica gel 110 NH2
Tedlar(R) Gas Sampling Bag
XAD16N
Amberlite(R) IR120 hydrogen form
xad-2
Activated magnesium silicate; Magnesium silicate
Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous
Dowex(R) 1X8-200 Ion Exchange Resin
QuEChERS; QuEChERS
SEPARATORY FUNNEL 250ML W/GLASS STOPCOCK, EA
HybridSPE(R)-PLus
QuEChERS
Supel(TM)-Inert Multi-Layer Foil
Silica gel
Sterile
no
Storage
2-8C
4 to 30Â°C, 0.15% Kathon CG/ICP Biocide
2 to 8Â°C, 20% Ethanol
2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0
4 to 30Â°C, 0.15% Kathon CG
4 to 30Â°C, 20% Ethanol or 70% Acetonitrile
2ËšC to 8ËšC, 20% ethanol
4 to 30Â°C, 0.05 M Sodium Acetate containing 20% Ethanol
4 to 8Â°C, 100% Isopropanol
4 to 8Â°C, 20% Ethanol
4 to 30Â°C
2 to 8Â°C
2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol
20 to 25Â°C, TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.
4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol
4 to 30Â°C, 20% Ethanol
4 to 30Â°C, in 20% Ethanol
2Â°C to 8Â°C in 20% ethanol
2Â°Câ€“8Â°C, 20% ethanol
4 to 30Â°C, 0,05% Kathon CG/ICP Biocide
4 to 30Â°C, 20% Ethanol (Q, DEAE), 20% Ethanol, 0.2 M Sodium Acetate (S)
4 to 30ËšC
4Â°C to 8Â°C
2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol
4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate
4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH
2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)
4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate
4Â°C to 30Â°C, 20% Ethanol
2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol
Size
250 mL
43 mm × 123 mm
1.25 X 1.25 X 3.5
3 X 6.5 X 6
System
Sepax C-Pro|Sefia|Sepax 2
Qty/CS
400 / Cs
Quality
with moisture indicator free of heavy metals
wet
dry
monodispersed
Theoretical Plates
> 43 000 m^-1
5000 m-1
> 42 000 m^-1
> 13000 m-1
> 48 000 m^-1
Pressure Limit
20 bar (2 MPa, 290 psi)
2 MPa
Recommended Flow Rate
5 ml/min
1 ml/min
Relative Humidity
20%-95% (non condensing)
UNSPSC Code
41115705
41115703
41121710
41115709
41115710
41115808
41113009
Relative Viscosity
5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)
Vapor Density
>1 (vs air)
<1 (vs air)
Volume
12 mL
250 µL
100 µL
2 mL
400 Âµl - 2 ml
5-20 ml
0.3 mL
1 mL
25 mL
22 mL
50 mL
0.5 mL
100-500 Âµl
2-6 ml
3 mL
6 mL
Qty/Pk
200 / Pk
Recommended Cleaning-in-Place Reagent
2 M NaCl solution
Recommended maximum operational flow velocity
300 cm/h
Stability
Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat
Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C
Stable
Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color
Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C
Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Scale
Lab|Process
Lab
Lab|Process development|Process
Lab|Process development
Volume_28
5-20 ml
400 Âµl - 2 ml
100-500 Âµl
2-6 ml
Pictogram
GHS07
GHS08
Solubility
It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be
pH Stability Cleaning-in-Place (CIP)
1 to 14
Wetted Materials
Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)
Stokes Radius
~ 10
Swelling factor
~1.7
Suitability
Autosampler compatibility:Agilent Agilent 1050/1090A Agilent 1100/1200 Agilent 7673A, 7683 Agilent 5890/7985A/6890Hitachi AS-1000, AS-2000, AS-4000 L7200/L7250LEAP Technologies CTC LC PAL, GC PAL CombiPAL HTC PAL/HTS PALPerkinElmer Autosystem/AS-2000/XL
Suitable for extracting 10-15 g food/agricultural sample. The high level of salts (e.g. magnesium sulfate) is to induce phase separation between the acetonitrile and aqueous sample layer.
Suitable for extracting ~10 g food/agricultural sample. The citrate is to buffer the extraction to pH 5.0 - 5.5. At this pH, most acid and base labile pesticides are stable.
Sanitization Conditions
2% Hibitane/20% Ethanol or with 70% Ethanol
Well Volume
8.5
Wetted Part
yes
no
Total binding capacity
~50 mg hIgG/mL resin
Type of ion exchanger
Strong anion exchanger (quaternary ammonium)
Strong cation exchanger (methyl sulfonate)
Typical Pressure Drop Over the Packed Bed
~ 2 MPa
~ 3 MPa
MP
>1600 C
Vapor Pressure
17 mmHg ( 20 °C)
Sample Loading Volume
Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]
WGK
1
3
nwg
2
Viscosity Max
15
Total Time of Preparation
< 30 min/6 preps
Particle Size in Methanol Max
163

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