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Temperature

45°C

Absorbance

0.02 AU max. at 330nm

Additives

Without: Calcium, magnesium, phenol red

Without: Calcium, magnesium

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For the removal and/or purification of serine proteases e.g. thrombin

Designed for use with a syringe, peristaltic pump, or chromatography system

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

For purification of DNA from small molecules by gel filtration

GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Average sample loading capacity

400 mg

50 mg

Assay Percent Range

99.8% min by GC

Bed Dimensions

16 x 100 mm

16 x 600 mm

7 x 25 mm

16 x 25 mm

26 x 600 mm

1.6 x 2.5 cm

10 x 100 mm

26 mm x 400 mm

6.4 x 100 mm

Bed Height With Two Adaptors

0 to 200 mm

58 mm - 106 mm

96 mm-156 mm

380 mm-650 mm

546 mm-606 mm

650 mm-950 mm

Application_4

Cell isolation

Chromatography system|Manual

Centrifugation

Chromatography system

Assay

=99%

Applications

gas chromatography (GC): suitable

LPLC: suitable

HPLC: suitable

Bed Volume

320

120

~ 2.4 mL

1 mL

5 mL

Bed Height

100 mm

600 mm

25 mm

300 mm

Bed Support

Net

Filter

Frit

Base Style

Flat Bottom

Insert Vial

Fused Conical

CarbonLoad

20%

18%

11%

15%

Binding Capacity/ml Chromatography Medium

At least 10 mg histidine-tagged protein

~ 13 mg ovalbumin

Up to 20 mg histidine-tagged protein

At least 40 mg histidine-tagged protein/mL resin

BioProcess resin

yes

Boiling Point

189°C

2230 C

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

At least 10 mg histidine-tagged protein/ml sedimented medium.

Autoclavable

Not Autoclavable

Autoignition Temp

~800 °F

800 °F

Bed Height With One Adaptor

0 mm-29 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

579 mm-609 mm

Application_5

Chromatography system|Manual

Buffer

Running Buffers

Capacity

1.8 meq/mL by wetted bed volume

1.1 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

4.7 meq/g by dry weight

Avoid Using

Chelating agents such as EDTA, EGTA , citrate

BedWeight

500 mg

Bore Size (mm)

CAS_NO

15461-27-5

39389-20-3

112926-00-8

104219-63-8

9003-70-7

69011-20-7

CAS Registry Number 1

7631-86-9

67-68-5

ChEBI

CHEBI:28262

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chemical Name or Material

Dimethylsulfoxide

Silica Gel

Chem AlertStorageSymbol

Gray

Chemical Stability

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Commonly used aqueous buffers

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Chemical Substance ID

329759181

329763462

Closure Color

Silver

Black

Blue

Closure Material

Plastic

Polypropylene

Aluminum

Polyethylene

Color

Colorless to Slightly Yellow

Grey

N/A

Color

Yellow

Amber

Black

Clear

Gold/Brown

Colorless

Gold

Silver

White

Brass

Natural

Red

Chromatography technique

Affinity coupling

Hydrophobic Interaction Chromatography

Ion Exchange Chromatography

Antibody affinity chromatography

GST-tagged protein purification

Histidine-tagged protein purification

Group specific affinity

ClosureType

Screw Top

Screw

Snap Cap

Screw Cap

Column i.d.

26 mm

50 mm

10 mm

16 mm

5 mm

Closure Type

Crimp Cap

Screw Cap

With Titeseal Open-bottom Plug Style Plastic Closures, Unattached

Screw

Density

1.100g/cmÂ³

Density Max.

1.2 g/ml

ClosureMaterial

Polypropylene

ClosureSize

11 mm

9 mm

Column volume

8 mL

1 mL

Closure Size

13 mm

20 mm

11 mm

9 mm

Column Type

packed GC

Reversed Phase

Mixed Mode

Column Capacity

< 1 mg/mL

Column Format

Analytical Column, EASY-Spray

Analytical Column

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Diameter Metric

2.1 mm

75 µm

4.6 mm

Diameter Metric Inner

12 mm

15 mm

6.6 mm

Composition

magnesium sulfate, 150 mg

Tris Base, Sodium Chloride

Decomposition Information

>190°C

Diameter Metric Opening

11 mm

Diameter Metric Outer

12 mm

8 mm

15 mm

Concentration

20 X

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Endotoxin Activity Max.

0.12 EU/mg

Flow Velocity

< 450 cm/h

< 150 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 300 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

<1800 cm/h

15 cm/h|15 cm/h

â‰¤ 600 cm/h

Flow Rate

< 4 mL/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

>10 ml/min

1.6 mL/min

2 ml/min - 10 ml/min

< 20 mL/min

< DarcyÂ´s Law

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

< 20 ml/min|< 20 ml/min

1 ml/min - 4 ml/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

COLOR

Blue

0.20 µm

Exclusion Limit Peptides

4 x 10Â³

Endcapped

Proprietary

Yes

Format_6

Prepacked column

Bulk resin

Prepacked columns

Kits

Form

powder or crystals

Liquid

powder

Diameter MetricOpening

9 mm

Filtrate Vessel Material

Polycarbonate (PC)

Finish

Plain top

Formula Weight

78.13

EC Number

231-545-4

200-838-9

231-153-3

For Use With

ELSD, UV Detector

Estimated Shelf Life from Manufacture Date

5 years

Feature

Endcapped

FilmThickness

1 µm

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Flash Point

87°C (188.6°F)

For Use With

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

Format

Liquid

matrix material

Formula

Service & Support

Formula Weight

60.08

Grade

High-purity Grade (Davisil Grade 646)

High-purity Grade (Davisil Grade 923)

H-Codes

H315-H319-H335

H317

H319

H373

H372

H351

Grade

technical grade

HPLC

high-purity grade

high-purity grade (Davisil Grade 62)

Graduated

Group To be Coupled

-NHâ‚‚

Guidance

For AKTAprime

Health Hazard 2

WARNING!

Height English

1.69 in.

1.57 in.

1.25 in.

Height English

1.77 in.

Graduations

0.5, 1, 1.5 mL

Graduations

0.5 mL

Identification

Pass Test

Height Metric

43 mm

45 mm

32 mm

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Hold-up Volume of Membrane

<5 Î¼l

<10 Î¼l

<20 Î¼l

Housing

Polypropylene (PP)

Height Metric

40 mm

Inlet id

2 mm (0.08 in)

0.75 mm (0.029 in)

1 mm (0.04 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

Ion Exchanger Type

Multimodal weak cation exchanger

Strong cation exchanger

Weak cation exchanger

Weak and strong anion

Weak anion exchanger

Strong anion exchanger

Weak anion

Ionic Capacity

0.13-0.17 mmol/ml

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.29-0.35 mmol Cl^-/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.18-0.25 mmol H⁺/ml medium

0.15-0.20 mmol H⁺/mlÂ

40-85 Âµmol/ml (Cl^-)

Length Metric

50 mm

51 mm

60 m

750 mm

250 mm

30 m

400 mm

150 mm

20 mm

Ligand Coupling Method

Dextran surface extenders

Reductive Amination

Ether linkages

Ether linkages and hydrophilic spacer arm

Epoxy

Maleimide activation

Cyanogen bromide activation

Triazine

Epoxy activation

Ligand Density

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

6 mg rProtein A/ml medium

3 mg Protein A/ml medium

~ 5 mg/mL resin

Approx. 13 Î¼mol/ml

3 mg GammaBind G, type 3/ml medium

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

Ligand_7

Protein A

Protein A, alkali stabilized

Protein G

2-mercaptopyridine

Protein L

Protein A, alkali stablilized

Liner Material

Aluminum/Silicone

PTFE/Rubber

PTFE/Silicone/PTFE

None

PTFE/Silicone

Polypropylene

Marking Spot

Yes

Material

aluminum liner

blunt tip Kel-F needle (point style 3)

polypropylene hardware

Acetal plastic (POM)

blue polypropylene cap

Brass

PEEK

Phenolic

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

polypropylene tube

Amber Glass (Type 51A)

Glass

glass body

PTFE Luer lock

bevel tip stainless steel needle (point style 2)

Cellulose

Fused Silica

PTFE/silicone septa

Silica Gel

VG2, 60% Vespel/40% Graphite

Mixed

PTFE/silicone septum

Clear Glass

Plastic/Stainless Steel

PTFE

red PTFE/silicone septum

Tedlar ® PVDF film

white PTFE/silicone

Borosilicate Glass

Polypropylene

Polypropylene (PP)

stainless steel TD tube

Polycarbonate (PC)

SilTite

stainless steel column

Material Septa

PTFE/Silicone

PTFE/Silicone/PTFE

Matrix

Rigid, cross-linked agarose, spherical

2% agarose

Cross-linked dextran

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Highly cross-linked agarose, spherical.

Cross-linked agarose, 4%

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

Composite of cross-linked agarose and dextran

Highly cross-linked 4% agarose

Highly cross-linked 6% agarose

Composite of cross-linked agarose

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

agarose, 6%, spherical

4% Agarose

4% cross-linked agarose

cross-linked agarose, 4%, spherical

cross-linked, 6% agarose particles

Max Pressure

17400 psi (1200 bar)

4500 psi (310 bar)

900 psi

14500 psi (1000 bar)

11600 psi (800 bar)

2000 psi

4000 psi (275 bar)

5800 psi (400 bar)

MDL Number

MFCD00132726

MFCD00145831

MFCD00145841

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00166483

MFCD00132712

MFCD00132707

MFCD00132771

MFCD00132722

MFCD00011232

Metal Ion Capacity

~15 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

Molecular Formula

O2Si

C2H6OS

Molecular Weight

84.93

100.39

0.0

60.08

Molecular Weight

78.129

Operating Flow Rate, Max

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

1.0 mL/min

2.6 mL/min

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

Operating Pressure Max

4.1 bar (60 psi)

Order Information

Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Allows safe, reliable storage and transport of self-packed Tricorn columns.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

HiLoad columns are prepacked XK columns

Percoll PLUS is for research use only.

For research use only.

HiLoad columns are prepacked XK columns.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Osmolality

276-298 mOsm/kg

288-310 mOsm/kg

P-CODES

P305 + P351 + P338

P280

P314

Pack size

1 kit

1.5 g

10 Ã— 2 mL

5 Ã— 1 mL

6 x 1 L

1000 mL

15 g

1 Ã— 120 mL

1 piece

20 mL

250 preps

300 mL

1 Ã— 1 mL

4.7 mL

5 Ã— 5 mL

50 preps

6 x 100 mL

75 mL

10 mL

200 mL

25 mL

4 x 50 mL

4 x 53 mL

500 g

6 x 500 mL

1.7 mL

100 mL

125 mL

25 g

100 g

5 mL

50 mL

1 L

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 Ã— 100 ÂµL

50 columns

6 mL

7 Ã— 1 mL

1 Ã— 4.7 mL

2 x 1 mL

500 mL

Pack Size

PackingMaterial

Mxt-502.2

Forte BP1

Partical Size Dry

50 Âµm-150 Âµm

>20 Âµm

18 Âµm-111 Âµm

40 Âµm-120 Âµm

Particle Size

40 to 60 µm

60 Î¼m-160 Î¼m

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

60 Âµm-165 Âµm

2 µm

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

24-44 Âµm

Particle size, d_50V

~ 8.6 Âµm

~50 Âµm

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~85 Âµm

~90 Âµm

34 Âµm

~34 Âµm

~34 Î¼m

~75 Âµm

~90 Î¼m

7.5 pH

7.0 to 7.4

pH ligand fully charged

Below 9

Above 6

Entire pH range

pH stability, CIP

2â€“12

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

2â€“14Co²⁺-stripped medium

1â€“14

2â€“14

2â€“10

3â€“14

3â€“10

4â€“13

2â€“13

2â€“13.7

3â€“12

pH stability, operational

3 to 12

3â€“11

3â€“13

2â€“9

4â€“13

4â€“9

3â€“12

3â€“9

2â€“13

4â€“12

2â€“12

2â€“10

pH 6 to 10

3â€“10

Physical Form

Liquid

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Polarity

Non-Polar

Non Polar

PoreSize

10 µm and 30 µm

130 Å

300 Å

40 to 60 µm

Pressure/Flow Specification

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

Product Line

nanoLC

20 mm Crimp Seal

Pierce

TraceGOLD

Easy Spray

ProductLine

Acclaim

Visiprep

Properties

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Quality

dry

Quantity

1/Pk.

500 mL

100

1000/Pk.

100/Pk.

Quantity

10 x 1 mL ampules

144 Pack

100 Pack

1000

1000/Cs.

Each

R-CODES

48/20

45-36/38

36/37/38

Resin Type

Superdex 200 Increase

Capto

Sepharose Fast Flow

MabSelect

Sepharose High Performance

RTECS Number

VV7315000

S-Codes

26-36

Sample Volume

0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)

25 to 500 ÂµL

< 0.5 mL

< 5 mL

< 100 Âµl

<= 15 ml

<= 5 ml

100 to 300 Î¼l

Up to 8.5 mL

600 Âµl

<= 13 ml

4 to 50 ÂµL

<= 1.5 ml

Septum

PTFE/Silicone/PTFE

None

Includes

3.5 in. Coil

Vials and Closures

Fritted disc, 2mm bore PTFE stopcock plug and 24/40 standard taper upper joint

Two (0.5 m and 2 m L) 1/16 in. OD PTFE tubing assembly with 1/4-28 UNF PP fitting at one end, a frit kit (contains two each of 10 µm PE, 10 µm PTFE, and 30 µm PTFE frits), and a Click-N-Seal 10-32 PEEK conical port fitting.

100 vials and 100 seals with septa

IUPAC Name

methylsulfinylmethane

Insert Type

250µL Fused Insert

Pre-Slit

350µL Fused Insert

Fused Insert

Ligand

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Native Protein A

TALONÂ® Superflow Cobalt-IMAC

Diethylaminoethyl

Alkaline-stabilized protein A-derived (E.coli).

Octylamine

Alkali-stabilized Protein A-derived (E. coli)

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Sulphopropyl

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Carboxymethyl

Porcine heparin

Cibacron Blue

Quaternary amine

Ligand Concentration

~4mg heparin/mL resin

~45 Âµmol phenyl/mL resin

4.5-8 mg gelatin/ml drained resin

~25 Âµmol phenyl/mL resin

Linear Formula

(C10H12 · C10H10 · C8H8)x

(CH₃)₂SO

SiO2

Length Metric

100 mm

457 mm

Material

N/A

Stainless Steel

100% Dimethyl Polysiloxane

Borosilicate Glass, PTFE, Polyethylene, FKM/FPM

Stainless steel Frit

Max Temperature

445°C

Sorbent

HyperSep Florisil

Includes

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Connectors for high- and low-pressure chromatography equipment and instructions.

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Instructions supplied with the concentractors

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Standard Concentration

98 to 100%

Surface Area

450 m 2 /g

480 m 2 /g

800 m 2 /g

300 m 2 /g

320 m 2 /g

=480 m 2 /g (approx.)

450 m²/g

170 m 2 /g

500 m 2 /g

530 m 2 /g

300 m²/g

100 m²/g

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Kit Contents

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL

RNAspin Lysis Solution (Red sticker): 25 ml, RNAspin Wash Buffer I (Yellow sticker): 15 ml, RNAspin Wash Buffer II (Grey sticker): 12.5 ml Add 50 ml Ethanol; RNAspin Desalting Buffer (Blue sticker); 25 ml, RNAspin Desalting Reaction Buffer (Green sticker)

RNAspin Lysis Solution (Red sticker): 125 ml, RNAspin Wash Buffer I (Yellow sticker): 80 ml, RNAspin Wash Buffer II (Grey sticker): 3 Ã— 25 ml Add 100 ml Ethanol to each bottle; RNAspin Desalting Buffer (Blue sticker); 125 ml, RNAspin Desalting Reaction B

Aprotinin (10 mg): 6500 M_r
Ribonuclease A (50 mg): 13 700 M_r
Carbonic anhydrase (15 mg): 29 000 M_r
Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Blue Dextran 2000

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

IOD-Codes

Max Temperature

340/360°C

40°C

330/350°C for Standard Columns; 430°C for Metal Columns

MarkingSpot

Yes

Inlet Connector

Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing

Ion exchanger

S (cation exchanger)

Q (anion exchanger)

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Min Temperature English

50°F

Length English Needle

2 in.

Matrix

6% Cross-Linked Agarose

Highly cross-linked agarose with dextran surface extender

Highly Cross-Linked Agarose

Needle Gauge

22 G

16 g

Operating Flow Rate, Recommended

1.3 mL/min

0.30 mL/min

0.45 mL/min

0.075 mL/min

0.80 mL/min

0.5 mL/min

Operating pH

1 - 9

2 - 9

0 - 14

2 - 7.5

2 - 8

Optical Activity

6.5 (5% in slurry)

Order code

SH30588.02

SH30031.FS

SH30031.02

SH30268.01

SH30588.01

Packaging Layout

2.5, 5, 25 kg in fiber drum

100, 500 g in poly bottle

5, 25 kg in fiber drum

2.5 kg in poly drum

1 kg in poly bottle

Packing Material

Silica

Spherical, Solid Core, Ultrapure Silica

Type B Silica

Spherical, Fully Porous, Ultrapure Silica

Spherical, Nanopolymer Hybrid Silica

Particle size distribution, wet

100â€“300 Âµm

20â€“80 Âµm

55â€“165 Âµm

87â€“510 Âµm

90â€“280 Âµm

15â€“88 Âµm

>1600 C

ParticleSize

3 µm

2.2 µm

5 µm

40 to 60 µm

Material Coating

Polypropylene

Material Plunger

Stainless Steel

Max Temperature Metric

50°C

pH Stability Cleaning-in-Place (CIP)

1 to 14

Max Temperature English

122°F

Pictogram

GHS08

GHS07

Needle PointNumber

Noper Pack

100

Operating Temperature

4Â°C-60Â°C

Pore Size

0.75 cm 3 /g pore volume

100 Å

150 Å

2 µm porosity

60 +?

60 Å mean pore size

70 Å pore size

90 Å pore size

110 Å pore diameter

0.8 cm 3 /g pore volume

100 Å

~0.55 mL/g pore volume

120 Å

90 Å

0.7-0.85 cm 3 /g pore volume (approx.)

Outer Diameter

10-21 mm

12 or 18 mm

p H

2.5 to 7

2.5 to 7.5

8.5 pH

7.5 pH

2 to 8

Porosity

10 Î¼m

20 Î¼m

10 or 80 Âµm

7 Î¼m

Pressure/flow characteristics

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

Synonyms

SUPELCOGEL(TM) 8% Crosslinked HPLC Column

QuEChERS

XAD16N

Temperature Range

-60°C to +340/360°C

Purity

>=99%

Titratable Acid

0.001mEq/g max.

Type

Crimp or Snap Closure

HPLC Column

Liquid Chromatography (LC)

Analytical Column

VialType

9 mm SureStop Vial (AVCS)

11 mm Crimp Top Vial

9 mm Target DP Vial

Volume

6 mL

100-500 Âµl

2 mL

0.5 mL

2-6 ml

400 Âµl - 2 ml

5-20 ml

Volume Metric Working

250 µL

1.8 mL

Volume MetricResidual

<6 µL

<170µL

Volume MetricUsable

4 mL

1.5 mL

Volume Range

0.5 - 200 µL

Volume_28

5-20 ml

400 Âµl - 2 ml

100-500 Âµl

2-6 ml

Water Flow Rate at 10 bar (145 psi)

Wetted Materials

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

WGK

nwg

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Shipping Dimensions - W in

Scale

Lab

Lab|Process development|Process

Lab|Process

Lab|Process development

Length English

18 in.

Ligand

Recombinant Protein A (E.coli)

Ligand Type

Monomeric C18

SMILES

CS(=O)C

No per Pack

100

No per Pack

1000

100

Osmolality

< 30 mOsm/kg

< 25 mOsm/kg

Storage

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

2Â° to 8Â° C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

2Â°Câ€“8Â°C, 20% ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

4Â°C to 8Â°C

2 to 8Â°C, 20% Ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

Particle size distribution, dry

20 â€“ 80 Âµm

Particle Shape

Spherical

Particle Size in Methanol Min

pH Range

8.9â€“9.9

8.5â€“9.5

5.5â€“7.5

Phase

Reversed Phase and Ion Exchange

Reversed Phase

Packaging Format

200 Tips/Rack, 2 Racks/Cs

Intrinsic Viscosity

~ 17

pH Stability Working Range

3â€“12

Outlet id

0.75 mm (0.029 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

Preslit

Yes

Physical State

liquid

liquidSuspension

Solid

Temperature

60°C

Pub Chem CID

679

Purity GradeNotes

Mobile phase for HPLC applications

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Needle Point Style

Blunt

Packaging

Amber Glass

Percent Purity

≥99.9%

Signal Word

Warning

PurityGrade Notes

Mobile phase for HPLC applications

Recommended maximum operational flow velocity

300 cm/h

Recommended Flow Rate

1 ml/min

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

Refractive Index

1.4770 to 1.4800 (at 25°C)

Melting Point

18.4°C

Vapor Density

<1 (vs air)

Viscosity Max

Sterilization

0.1 Âµm sterile filtered

Storage Conditions

4ËšC to 30ËšC, 20% ethanol

4 - 30Â°C

4â€“30Â°C

At room temperature. Precipitation can occur at low temperature.

Volume Metric Usable

1.5 mL

Wetted Part

yes

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Stability

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Septum

Red PTFE/White Silicone

Support System

Polypropylene (PP)

Swelling factor

~1.7

Stationary Phase

Reversed Phase/Anion Exchange/Cation Exchange

C18

Solubility Information

Solubility aq. soln.: insoluble.

Particle Size in Methanol Max

163

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Pressure Limit

20 bar (2 MPa, 290 psi)

Volume Metric Residual

<170µL

Residueafter Evaporation

5ppm max.

Shelf Life

24 months

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Vent

On inlet

Volume MetricTotal

2 mL

Vapor Pressure

17 mmHg ( 20 °C)

0.55mbar at 20Â°C

Volume English

1 dr.

Total Time of Preparation

< 30 min/6 preps

Stokes Radius

~ 10

Theoretical Plates

> 13000 m-1

> 48 000 m^-1

5000 m-1

> 38 000 m^-1

> 43 000 m^-1

Suitability

Autosampler compatibility:Agilent Agilent 1050/1090A Agilent 1100/1200 Agilent 7673A, 7683 Agilent 5890/7985A/6890Hitachi AS-1000, AS-2000, AS-4000 L7200/L7250LEAP Technologies CTC LC PAL, GC PAL CombiPAL HTC PAL/HTS PALPerkinElmer Autosystem/AS-2000/XL

U SPType

USP Type

Viscosity

1.98 mPa/s at 25Â°C

Volume Metric

4 mL

Volume Metric Total

2 mL

System

Sepax C-Pro|Sefia|Sepax 2

Synonym

DMSO

Volume Metric

2 mL

2.0 mL

Water

0.04% max.

Thread Size

9 mm

10-425

20 mm

Total binding capacity

~50 mg hIgG/mL resin

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