Government Scientific Source

$0~$10(80)
$10~$25(189)
$25~$50(699)
$50~$75(595)
$75~$100(505)
$100~$200(1166)
$200~$300(821)
$300~$400(470)
$400~$500(397)
$500~$750(805)
$750~$1000(731)
$1000~$5000(1431)
$5000~$10000(152)
$10000~$50000(165)
$50000~$100000(23)
$100000~$3000000(13)

Temperature

45°C

Absorbance

0.02 AU max. at 330nm

Additives

Without: Calcium, magnesium, phenol red

Without: Calcium, magnesium

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For the removal and/or purification of serine proteases e.g. thrombin

Designed for use with a syringe, peristaltic pump, or chromatography system

Entry level chromatography solution for the preparative purification of proteins in lab scale.

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Average sample loading capacity

400 mg

50 mg

Assay Percent Range

99.8% min by GC

Applications

solid phase extraction (SPE): suitable

gas chromatography (GC): suitable

LPLC: suitable

HPLC: suitable

HPTLC: suitable

thin layer chromatography (TLC): suitable

Bed Dimensions

16 x 100 mm

16 x 600 mm

7 x 25 mm

16 x 25 mm

26 x 600 mm

1.6 x 2.5 cm

Bed Height With Two Adaptors

0 to 200 mm

Application_4

Cell isolation

Chromatography system|Manual

Assay

=99%

Bed Volume

320

120

1 mL

5 mL

Bed Height

100 mm

600 mm

25 mm

Bed Support

Net

Filter

Base Style

Flat Bottom

CarbonLoad

20%

15%

Binding Capacity/ml Chromatography Medium

At least 10 mg histidine-tagged protein

BioProcess resin

yes

Boiling Point

189°C

2230 C

Buffer Selection

2 buffer inlets

Capacity

1.3 meq/mL by wetted bed volume

1.1 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

=25 % absorption capacity (water)

4.7 meq/g by dry weight

1.7 meq/mL by wetted bed volume

Capacity

Flow: 2,500 mL/min @ 29 in. Hg

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

Autoclavable

Not Autoclavable

Application_5

Chromatography system|Manual

Autoignition Temp

800 °F

BedWeight

500 mg

Bore Size (mm)

CAS_NO

39389-20-3

75-09-2

9052-95-3

112926-00-8

104219-63-8

1343-88-0

7631-86-9

67-56-1

9003-70-7

142-82-5

69011-20-7

1344-28-1

CAS Registry Number 1

7631-86-9

67-68-5

ChEBI

CHEBI:28262

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chemical Name or Material

Dimethylsulfoxide

Silica Gel

Chem AlertStorageSymbol

Gray

Chemical Stability

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Commonly used aqueous buffers

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Chemical Substance ID

24857358

329755080

329759181

24852837

329763462

329763461

24870506

329750331

329759187

Chemical/Physical Resistance

Temp. Limits:. -10 C to 180 C isothermal and programmed

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Closure Color

Silver

Black

Blue

Closure Material

Plastic

Polypropylene

Aluminum

Polyethylene

Color

Colorless to Slightly Yellow

Grey

N/A

Color

Yellow

natural

Amber

Black

Clear

Gold/Brown

Green

Tan

Colorless

Blue/Tan

Gold

Silver

red band

White

Brass

Silver/Gray

Natural

Orange

Red

orange

Graphite

Multi

Chromatography technique

Affinity coupling

Hydrophobic Interaction Chromatography

Ion Exchange Chromatography

Antibody affinity chromatography

GST-tagged protein purification

Histidine-tagged protein purification

Group specific affinity

ClosureType

Screw Top

Screw

Snap Cap

Screw Cap

Column i.d.

26 mm

50 mm

10 mm

16 mm

5 mm

Column Type

capillary extremely polar

capillary chiral

capillary non-polar

packed GC

Reversed Phase

SPE

Mixed Mode

capillary polar

Closure Type

Crimp Cap

Screw Cap

With Titeseal Open-bottom Plug Style Plastic Closures, Unattached

Screw

Density

1.100g/cmÂ³

Density Max.

1.2 g/ml

ClosureMaterial

Polypropylene

ClosureSize

11 mm

9 mm

Composition

Discovery ® DSC-18, 400 mg

bed wt., 500 mg

magnesium sulfate, 150 mg

Tris Base, Sodium Chloride

Column volume

8 mL

1 mL

Description

For gas: Inert carrier, He, H2, N2, AR, Air

Closure Size

13 mm

20 mm

11 mm

9 mm

Column Capacity

< 1 mg/mL

Column Format

Analytical Column, EASY-Spray

Analytical Column

Diameter

11 mm

Diameter Metric

2.1 mm

75 µm

4.6 mm

Diameter Metric Inner

12 mm

15 mm

6.6 mm

Column Phase

ENVI-Carb

Decomposition Information

>190°C

Diameter Metric Opening

11 mm

Diameter Metric Outer

12 mm

8 mm

15 mm

Compatibility

Compatible with most QuEChERS shakers.

Concentration

20 X

EC Number

215-681-1

238-878-4

215-691-6

215-609-9

200-659-6

266-046-0

231-545-4

262-373-8

200-838-9

231-153-3

205-563-8

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Endotoxin Activity Max.

0.12 EU/mg

Feature

No Flourescent Indicator

Fluorescent Indicator

septum type Thermogreen ® LB-2

closure type black phenolic solid top cap

without needle Luer tip

closure type screw top vial

needle type N (cemented)

Not Endcapped

Endcapped

syringe type Sample Lock

preconditioned

Flow Velocity

< 450 cm/h

< 150 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 300 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

<1800 cm/h

15 cm/h|15 cm/h

â‰¤ 600 cm/h

Flow Rate

< 4 mL/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

>10 ml/min

0.001 to 25 mL/min (normal range), 0.01 to 50 mL/min (column packing flow)

1.6 mL/min

2 ml/min - 10 ml/min

< 20 mL/min

< DarcyÂ´s Law

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

< 20 ml/min|< 20 ml/min

1 ml/min - 4 ml/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

COLOR

Blue

0.20 µm

0.12 µm

For Use With Application

GC/LC

Food and Beverages

TLC

Exclusion Limit Peptides

4 x 10Â³

Endcapped

Proprietary

Yes

For Use With

For Use With AKTA start

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

For Use With Equipment

Click-On Inline Filter

Inert Carrier, He, H2, N2, AR

Inert Carrier, He, H2, N2, AR, Air

Form

fine crystals

powder or crystals

Liquid

fine powder

powder

Format_6

Prepacked column

Bulk resin

Prepacked columns

Kits

For use with1

AKTA avant and AKTA pure systems

Formula

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

MgO₃Si

CH₃(CH₂)₅CH₃

CH₃OH

O2Si

Service & Support

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

Diameter MetricOpening

9 mm

Dimensions_29

300 Ã— 360 Ã— 575 mm (W Ã— H Ã— D)

Filtrate Vessel Material

Polycarbonate (PC)

Finish

Plain top

Formula Weight

78.13

Flow Cell Path Length

2 mm (optional 0.5 mm and 10 mm)

2 mm (optional 5 mm)

For Use With

ELSD, UV Detector

Estimated Shelf Life from Manufacture Date

5 years

FilmThickness

1 µm

Flow Direction

Supported bidirectionally. Certain applications may require orientation, i.e., vents. Reverse flow only for low pressure applications.

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Empirical Formula

MgO3Si

Flash Point

87°C (188.6°F)

Format

Liquid

matrix material

Formula Weight

100.39

60.08

Grade

Standard

Certified Reference Material

Puriss. p.a.

technical grade

high-purity grade (Davisil Grade 643)

HPLC

Trace CERT ®

HPLC Gradient

high-purity grade

H-Codes

H371

H315-H319-H335

H317

H319

H373

H372

H351

Grade

High-purity Grade (Davisil Grade 646)

High-purity Grade (Davisil Grade 633)

High-purity Grade (Davisil Grade 923)

Guidance

The ends: Plug FCC - RJ45 round.

For AKTAprime

The kit contains 1 x Fill port 6 x Needle 1 x Syringe holder *The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100 % Ethyl acetate, 100 % Hexane and 100 % Tetrahydrofuran.

Tubing and connectors have to be ordered separately.

The kit contains 1 x Inlet check valve 1 x Outlet check valve *The wetted parts in the check valv kit are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100 % Ethyl acetate, 100 % Hexane and 100 %

To connect the air sensor use: Tubing i.d. 0.5 mm o.d. 1/16" Fingertight connector 1/16" Adapter for air sensor

To connect the air sensor use: Tubing i.d. 1.6 mm o.d. 1/8" Tubing connector 1/8" with Ferrule 1/8" Adapter for air sensor

Lamp and aperture are supplied with Hg lamp and housing

The tubing is fitted with 2 x Nut UNF 5/16" male 2 x Ferrule *The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100 % Ethyl acetate, 100 % Hexane and 100 % Tetrahydrofuran.

Graduated

Group To be Coupled

-NHâ‚‚

Health Hazard 2

WARNING!

Height

210 mm

457 mm (approx.)

660 mm

629 mm

Height English

1.69 in.

23.62 in.

1.57 in.

1.25 in.

Height English

1.77 in.

Graduations

0.5, 1, 1.5 mL

Graduations

0.5 mL

Handle Material

Stainless steel

Identification

Pass Test

Height Metric

43 mm

45 mm

32 mm

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Hold-up Volume of Membrane

<5 Î¼l

<10 Î¼l

<20 Î¼l

Housing

Polypropylene (PP)

Ion Exchanger Type

Strong cation exchanger

Weak cation exchanger

Weak anion exchanger

Strong anion exchanger

Weak anion

Length Metric

50 mm

250 mm

30 m

150 mm

Ligand Density

2 mg Protein G/ml medium

6 mg rProtein A/ml medium

~ 5 mg/mL resin

3 mg GammaBind G, type 3/ml medium

Ligand_7

Protein A

Protein A, alkali stabilized

Protein G

Protein L

Material

polypropylene hardware

Graphite

Brass

PEEK

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

polypropylene tube

VG1, 85% Vespel/15% Graphite

Glass

glass body

PE frit (20 µm porosity)

Aluminum Support

bevel tip stainless steel needle (point style 2)

Fused Silica

Silica Gel

silica gel 60 matrix

Vespel

VG2, 60% Vespel/40% Graphite

Aluminum

glass support

clear glass vial

Mixed

PTFE/silicone septum

Plastic/Stainless Steel

Polyetheretherketone (PEEK)

PTFE

Tedlar ® PVDF film

Borosilicate Glass

Polypropylene

Polypropylene (PP)

PTFE frit

glass TD tube

Polycarbonate (PC)

SilTite

Vespel/Graphite, VG1

Metal

stainless steel column

Steel

Steel/Plastic

Vespel/Graphite

Material Septa

PTFE/Silicone

PTFE/Silicone/PTFE

Matrix

Rigid, cross-linked agarose, spherical

Cross-linked dextran

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

Composite of cross-linked agarose and dextran

Highly cross-linked 4% agarose

Composite of cross-linked agarose

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

cross-linked agarose, 4%, spherical

cross-linked, 6% agarose particles

Max Pressure

17400 psi (1200 bar)

4500 psi (310 bar)

900 psi

11600 psi (800 bar)

MDL Number

MFCD00132726

MFCD00145831

MFCD00145841

MFCD00009544

MFCD00078249

MFCD00146442

MFCD00004595

MFCD00132707

MFCD00132722

MFCD00011232

Metal Ion Capacity

~15 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

Molecular Formula

O2Si

C2H6OS

Molecular Weight

78.129

Operating Pressure Max

4.1 bar (60 psi)

5 MPa (725 psi)

20 MPa (2900 psi)

10 bar (1 MPa)

P-CODES

P305 + P351 + P338

P280

P314

Pack Size

Pack size

5 Ã— 1 mL

1000 mL

1 piece

20 mL

300 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

10 mL

200 mL

25 mL

500 g

6 x 500 mL

100 mL

100 g

5 mL

50 mL

1 L

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 Ã— 100 ÂµL

50 columns

6 mL

7 Ã— 1 mL

1 Ã— 4.7 mL

2 x 1 mL

500 mL

Packaging Layout

fibre case

2.5, 5, 25 kg in fiber drum

100, 500 g in poly bottle

1, 6x1, 2.5 kg in poly bottle

1 kg in poly bottle

1 ft3 in fiber drum

25, 500 g in poly bottle

Partical Size Dry

50 Âµm-150 Âµm

18 Âµm-111 Âµm

40 Âµm-120 Âµm

Particle Size

60 Î¼m-160 Î¼m

24 Âµm-44 Âµm

60 Âµm-165 Âµm

2 µm

45 Âµm-165 Âµm

45 Î¼m-165 Î¼m

24-44 Âµm

Particle size, d_50V

~ 8.6 Âµm

~ 9 Âµm

~85 Âµm

~90 Âµm

34 Âµm

~34 Âµm

~34 Î¼m

~75 Âµm

~90 Î¼m

pH ligand fully charged

Below 9

Above 6

Entire pH range

pH stability, operational

3 to 12

3â€“13

2â€“9

4â€“13

4â€“9

2 to 12

3â€“12

3â€“9

2â€“13

4â€“12

2â€“12

2â€“10

pH 6 to 10

3â€“10

Physical Form

Liquid

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Pore Size

1000 Å

0.75 cm 3 /g pore volume

180 Å

100 Å

150 Å

90 Å mean pore size

60 Å

60 +?

60 Å mean pore size

70 Å pore size

0.7-0.9 cm 3 /g pore volume

30 Å

300 Å

90 Å pore size

160 Å

110 Å pore diameter

60 Å pore size

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

100 Å

~0.55 mL/g pore volume

120 Å

60 Å medium pore diameter

90 Å

0.7-0.85 cm 3 /g pore volume (approx.)

ProductLine

Acclaim

Properties

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Protection Class

IP 21

Quality

dry

with fluorescent indicator 254 nm

monodispersed

with moisture indicator free of heavy metals

Quantity

1/Pk.

500 mL

100

1000/Pk.

100/Pk.

100 g

30/Pk.

Quantity

144 Pack

50 g

10 g

100 Pack

1000

1000/Cs.

Each

200

R-CODES

48/20

36/37/38

Relative Humidity

20% to 95% (noncondensing)

20%-95% (non condensing)

RTECS Number

VV7315000

S-Codes

26-36

Sample Volume

25 to 500 ÂµL

< 0.5 mL

< 5 mL

<= 15 ml

<= 5 ml

600 Âµl

<= 13 ml

4 to 50 ÂµL

<= 1.5 ml

Ionic Capacity

0.11â€“0.16 mmol (Cl^-)/mL resin

0.09-0.13 mmol H⁺/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.18-0.25 mmol H⁺/ml medium

IUPAC Name

methylsulfinylmethane

Ligand

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

TALONÂ® Superflow Cobalt-IMAC

Diethylaminoethyl

Alkali-stabilized Protein A-derived (E. coli)

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Sulphopropyl

Glutathione and 10-carbon linker arm

Phenyl

Carboxymethyl

Cibacron Blue

Quaternary amine

Linear Formula

(C10H12 · C10H10 · C8H8)x

(CH₃)₂SO

MgO3Si

Length Metric

100 mm

Ligand Coupling Method

Ether linkages

Maleimide activation

Cyanogen bromide activation

Epoxy activation

Material

N/A

Multiple

Stainless Steel

Stainless Steel Hardware

Borosilicate Glass, PTFE, Polyethylene, FKM/FPM

Injection volume1

Programmable from 0 to 5000 ÂµL 1 ÂµL increments. Maximum injection volume depends on installed sample loop and injection mode

Surface Area

289 m 2 /g

450 m 2 /g

480 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

300 m 2 /g

470-530 m 2 /g

22 m 2 /g

320 m 2 /g

450 m 2 /g

~500 m 2 /g

=480 m 2 /g (approx.)

200 m 2 /g

430-530 m 2 /g

475 m 2 /g

80 m 2 /g

450 m²/g

170 m 2 /g

100 m 2 /g

500 m 2 /g

250-350 m 2 /g

530 m 2 /g

300 m²/g

100 m²/g

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand Concentration

~45 Âµmol phenyl/mL resin

Includes

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Connectors for high- and low-pressure chromatography equipment and instructions.

Instructions supplied with the concentractors

Injection valve

1/16â€ PEEK valve 6 port 2 position with 0.25 mm i.d. bore

Injections per vial/well

Max. 9

Inlet id

0.75 mm (0.029 in)

1 mm (0.04 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

IOD-Codes

Molecular Weight

100.20

100.39

0.0

60.08

Max Temperature

40°C

Multiple Sample Injection

Upgradeable to 5 samples

Yes, up to 7 samples

MarkingSpot

Yes

Ion exchanger

S (cation exchanger)

Q (anion exchanger)

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Includes

Two (0.5 m and 2 m L) 1/16 in. OD PTFE tubing assembly with 1/4-28 UNF PP fitting at one end, a frit kit (contains two each of 10 µm PE, 10 µm PTFE, and 30 µm PTFE frits), and a Click-N-Seal 10-32 PEEK conical port fitting.

Length

2 m

Length English Needle

2 in.

Matrix

6% Cross-Linked Agarose

Highly cross-linked agarose with dextran surface extender

Highly Cross-Linked Agarose

Operating Flow Rate, Recommended

1.3 mL/min

0.075 mL/min

0.80 mL/min

0.5 mL/min

Operating pH

1 - 9

2 - 9

0 - 14

2 - 7.5

3.5 - 7.0

3.8 - 6.8

2 - 8

Operating temperature1

4 Â°C to 40 Â°C; indoor use only

Optical Activity

6.5 (5% in slurry)

~7 (5% in H 2 O, suspension)

7.0 (5% in slurry)

>1600 C

Liner Material

PTFE/Silicone

ParticleSize

3 µm

5 µm

pH Monitoring

0 to 14 (optional)

0â€“14

pH Stability Cleaning-in-Place (CIP)

1 to 14

0 to 14

pH stability, CIP

2â€“9

2â€“14Co²⁺-stripped medium

1â€“14

2â€“14

2â€“10

3â€“14

3â€“10

4â€“13

2â€“13

2â€“13.7

3â€“12

Pictogram

GHS08

GHS07

Noper Pack

100

Operating Temperature

<40Â°C

4Â°C-35Â°C

PoreSize

300 Å

Order Information

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Percoll PLUS is for research use only.

For research use only.

HiLoad columns are prepacked XK columns.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

p H

2.5 to 7

7.5 pH

2 to 8

Porosity

10 Î¼m

20 Î¼m

10 or 80 Âµm

7 Î¼m

Pressure/flow characteristics

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

Pressure/Flow Specification

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

Product Line

Pierce

Easy Spray

Supelclean ENVI

Purity

>=99%

Titratable Acid

0.001mEq/g max.

Volume

22 mL

3 mL

50 mL

6 mL

100-500 Âµl

2 mL

25 mL

1 mL

50 µL

0.5 mL

2-6 ml

400 Âµl - 2 ml

2.5-100 µL

5-20 ml

Volume MetricUsable

4 mL

1.5 mL

Volume_28

5-20 ml

400 Âµl - 2 ml

100-500 Âµl

2-6 ml

Wetted Materials

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

Polyetheretherketone (PEEK)

Ethylenetetra fluoroethylene (ETFE)

Polyetheretherketone (PEEK)/ Fluorinated Ethylene Propylene (FEP)/ PolyChloroTriFluoroEthylene (PCTFE)

Polyetheretherketone (PEEK)/Ethylenetetrafluoroethylene (ETFE)

Polyetheretherketone (PEEK)/Fluoroethenepropene (FEP)/Ethylenetetrafluoroethylene (ETFE)

Polyetheretherketone (PEEK)/Polytetrafluoroethene (PTFE)

WGK

nwg

Relative humidity1

20% to 80% RH

Scale

Lab

Lab|Process development|Process

Lab|Process

Lab|Process development

Multiple Wavelength Detection

Yes, up to 3 wavelengths

Up to 3 wavelengths

Ligand

Recombinant Protein A (E.coli)

SMILES

CS(=O)C

No per Pack

100

Osmolality

< 30 mOsm/kg

< 25 mOsm/kg

Multi-Step Purification

Yes, (optional)

Number of Ports

Storage

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

2Â°Câ€“8Â°C, 20% ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2-8C

4Â°C to 8Â°C

2 to 8Â°C, 20% Ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

Particle Shape

Spherical

Particle Size in Methanol Min

pH Range

8.9â€“9.9

8.5â€“9.5

5.5â€“7.5

Phase

Reversed Phase and Ion Exchange

Reversed Phase

Intrinsic Viscosity

~ 17

pH Stability Long Term

43843

Power Consumption

300 VA

800 VA

Power requirements

95 to 240 Volt AC Â± 10%; 50 to 60 Hz; 200 VA

Operating Flow Rate, Max

1.0 mL/min

2.6 mL/min

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

Outlet id

0.75 mm (0.029 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

Packing Material

Spherical, Fully Porous, Ultrapure Silica

Spherical, Nanopolymer Hybrid Silica

pH Stability Short Term

1â€“14

Preslit

Yes

Physical State

liquid

liquidSuspension

Solid

Temperature

60°C

Pub Chem CID

679

Purity GradeNotes

Mobile phase for HPLC applications

No per Pack

100

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Output

4 W

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Needle Point Style

Blunt

Packaging

Amber Glass

Percent Purity

≥99.9%

Signal Word

Warning

Danger

7.0 to 7.4

PurityGrade Notes

Mobile phase for HPLC applications

Recommended maximum operational flow velocity

300 cm/h

Recommended Flow Rate

1 ml/min

Residue After Evaporation

5ppm max.

Resin Type

Capto

Sepharose Fast Flow

MabSelect

Sepharose High Performance

RIDADR

NONH for all modes of transport

Refractive Index

1.4770 to 1.4800 (at 25°C)

Synonyms

Activated magnesium silicate; Magnesium silicate

QuEChERS

Silica gel

TLC cards Silica gel

QuEChERS; QuEChERS

Tedlar(R) Gas Sampling Bag

XAD16N

Safety certifications1

CE, cMETus, EU ROHS, China ROHS

Melting Point

18.4°C

Type

HPLC Column

Liquid Chromatography (LC)

Analytical Column

SPE Column

Ferrule

Vapor Density

<1 (vs air)

Viscosity Max

Sterilization

0.1 Âµm sterile filtered

May be autoclaved at 121Â°C for 20 minutes. Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed after autoclavin

Storage Conditions

4ËšC to 30ËšC, 20% ethanol

Store at room temperature.

4 - 30Â°C

4â€“30Â°C

At room temperature. Precipitation can occur at low temperature.

Volume Metric Usable

1.5 mL

Weight

Up to 53

Wetted Part

yes

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Stability

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Surface Area Metric

100 m2/g

Storage conditions1

-25 Â°C to 60 Â°C

Safety Certifications

2006/42/EC (MD), 2004/108/EC (EMC), 1999/5/EC (R&TTE)

Sampler capacity

Vial rack with 108 positions for (1.5 mL). Microplates according to SBS standards. 96-well (deep-well and standard) as well as 384-well

Sealing

Heat-fused

Septum

None

Septum

Red PTFE/White Silicone

Shape

Round

Support System

Polypropylene (PP)

Swelling factor

~1.7

Stationary Phase

Reversed Phase/Anion Exchange/Cation Exchange

C18

Pump Type

Piston pump, metering type

Osmolality

288-310 mOsm/kg

Solubility Information

Solubility aq. soln.: insoluble.

Particle Size in Methanol Max

163

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Pressure Limit

20 bar (2 MPa, 290 psi)

Voltage

100 to 120/220 to 240 VAC

100-120/220-240 VAC

Volume Metric Residual

<170µL

Width

215

860

535

Residueafter Evaporation

5ppm max.

Sample loop

Default 100 ÂµL

Shelf Life

24 months

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

UV Wavelength

190 to 700 nm

190-700 nm

Vent

On inlet

Volume MetricTotal

2 mL

Syringe volume

500 ÂµL standard; 2500 ÂµL optional

Vapor Pressure

17 mmHg ( 20 °C)

0.55mbar at 20Â°C

Volume English

1 dr.

Total Time of Preparation

< 30 min/6 preps

Stokes Radius

~ 10

Theoretical Plates

> 13000 m-1

> 48 000 m^-1

5000 m-1

> 38 000 m^-1

> 43 000 m^-1

Suitability

Autosampler compatibility:Agilent Agilent 1050/1090A Agilent 1100/1200 Agilent 7673A, 7683 Agilent 5890/7985A/6890Hitachi AS-1000, AS-2000, AS-4000 L7200/L7250LEAP Technologies CTC LC PAL, GC PAL CombiPAL HTC PAL/HTS PALPerkinElmer Autosystem/AS-2000/XL

U SPType

Volume Metric

6 mL

2 mL

2.0 mL

USP Type

Viscosity

1.98 mPa/s at 25Â°C

0.35 to 10.0 cP (system pump)

Volume Metric

4 mL

Volume Metric Total

2 mL

System

Sepax C-Pro|Sefia|Sepax 2

Volume MetricResidual

<170µL

Weight_30

21 kg

Synonym

DMSO

Water

0.04% max.

Thread Size

9 mm

10-425

20 mm

Total binding capacity

~50 mg hIgG/mL resin

VialType

11 mm Crimp Top Vial

9 mm Target DP Vial

Wetted part1

PEEK, PTFE, Tefzelâ„¢, Vespelâ„¢, PCTFE, glass. Needle coated with silica on stainless steel SS316 (inside and outside)

Volume Metric Working

1.8 mL

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