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Additional Information

Vapor Pressure: Negligible

Absorption Ratio

1.21 to 1.7

Bio StainCommCertification

Pass Test

Beilstein Reference

27, V,19, 259

3599847

CAS Registry Number 1

314-13-6

34722-90-2

6104-58-1

6586-05-6

7220-79-3

76-59-5

72-57-1

76-61-9

CAS Registry Number 4

6586-05-6

CAS RN 3 Min Percentage

0.76

CAS RN 4 Max Percentage

0.45

CAS_NO

34722-90-2

115-39-9

76-59-5

620-45-1

76-61-9

7732-18-5

518012-62-9

CAS RN 2 Max Percentage

1.39

CAS RN 2 Min Percentage

1.34

CAS RN 4 Min Percentage

0.43

CAS RN 1 Max Percentage

99.0

Chemical Name or Material

Evans Blue

Thymol Blue

Methylene Blue

Bromothymol Blue

Coomassie Brilliant Blue G-250 [for Electrophoresis]

Methylene Blue trihydrate

Trypan Blue

New Methylene Blue “N”

Chem Alert Storage Symbol

Gray

Chemical Substance ID

24847172

24883329

Color

Brown to Green

Brown-Pink to Purple

Purple-Red

COLOR

Green

Color IndexNo

52015

Concentration

0.04%

Content AndStorage

Store at room temperature.

Density

1.230g/cm-?

13 (Air = 1.0)

Concentrationor CompositionbyAnalyteorComponents

1% (w/v) Aqueous Solution

Detection Location

In-Gel Detection, In-Solution Detection, In-Blot Detection

EINECS Number

(5)-1656

200-973-3

200-515-2

200-971-2

206-242-5

200-786-7

Description

Pierce Coomassie Brilliant Blue G-250 Dye

Dye Content

82% min.

Report %

Detection Method

Colorimetric

Formula Weight

319.85

373.9

960.82

646.37

854.02

466.58

Formula Weight

624.38

373.90 (319.86 Anhydrous)

Formula

C₂₇H₃₀O₅S

C₂₇H₂₈Br₂O₅S

C₂₇H₂₇Br₂NaO₅S

C12H6Cl2NNaO2 * 2 H2O

C₁₉H₁₀Br₄O₅S

Grade

Certified Biological Stain

Pure

ACS Reagent

Health Hazard 2

GHS H Statement:
Harmful if swallowed.

WARNING!

GHS H Statement
H351

GHS H Statement
H302
Harmful if swallowed.

GHS H Statement
H350
May cause cancer.

Health Hazard 1

H302

H350

Warning

Health Hazard 3

Emergency Overview
Harmful if swallowed. Causes eye, skin, and respiratory tract irritation. May cause methemoglobinemia. Ensure adequate ventilation. Use personal protective equipment. Wash off immediately with plenty of water for at least 15

P264b-P270-P301+P312-P330-P501c

GHS P Statement:
IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.

WARNING: The information provided on this web site was developed in compliance with European Union (EU) regulations and is correct to t

P201-P202-P281-P308+P313-P501c

InChIKey

GGKWUSWRGRCLEV-UHFFFAOYSA-K

NMKFVGALBGZKGW-FKWCIMQXSA-M

KBNIFDASRCWYGC-UHFFFAOYSA-J

PRZSXZWFJHEZBJ-UHFFFAOYSA-N

WMYVHJWZUUEZNE-ARWFNKCKSA-J

RWVGQQGBQSJDQV-UHFFFAOYSA-M

NUHCTOLBWMJMLX-UHFFFAOYSA-N

XQAXGZLFSSPBMK-UHFFFAOYSA-M

IUPAC Name

[7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;chloride

[7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;chloride;trihydrate

2-bromo-4-[3-(3-bromo-4-hydroxy-2-methyl-5-propan-2-ylphenyl)-1,1-dioxo-2,1$l^{6}-benzoxathiol-3-yl]-3-methyl-6-propan-2-ylphenol

sodium;2-bromo-4-[(Z)-(3-bromo-2-methyl-4-oxo-5-propan-2-ylcyclohexa-2,5-dien-1-ylidene)-(2-sulfophenyl)methyl]-3-methyl-6-propan-2-ylphenolate

4-[3-(4-hydroxy-2-methyl-5-propan-2-ylphenyl)-1,1-dioxo-2,1$l^{6}-benzoxathiol-3-yl]-5-methyl-2-propan-2-ylphenol

sodium;3-[[4-[(Z)-[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]-2-methylcyclohexa-2,5-dien-1-ylidene]methyl]-N-ethyl-3-methylanilino]methyl]benzenesulfonate

tetrasodium;(3Z)-5-amino-3-[[4-[4-[(2Z)-2-(8-amino-1-oxo-3,6-disulfonatonaphthalen-2-ylidene)hydrazinyl]-3-methylphenyl]-2-methylphenyl]hydrazinylidene]-4-oxonaphthalene-2,7-disulfonate

tetrasodium;4-amino-6-[[4-[4-[2-(8-amino-1-oxo-5,7-disulfonatonaphthalen-2-ylidene)hydrazinyl]-3-methylphenyl]-2-methylphenyl]hydrazinylidene]-5-oxonaphthalene-1,3-disulfonate

zinc(2+) 3,7-bis(ethylamino)-2,8-dimethyl-5++G??-phenothiazin-5-ylium trichloride

MDL Number

MFCD00005869

MFCD00005872

MFCD00005875

MFCD06200731

MFCD00077263

Infrared Spectrum

Authentic

Melting Point

204.0°C

>300°C

190°C

190.0°C

221.0°C

MFCD Number

MFCD00003969

MFCD00005869

MFCD00005872

MFCD00150008

MFCD00004021

MFCD00012111

MFCD00077263

MFCD00078482

Molecular Formula

C18H22Cl3N3SZn

C27H28Br2O5S

C34H24N6Na4O14S4

C27H27Br2NaO5S

C27H30O5S

C47H48N3NaO7S2

C16H24ClN3O3S

Labelor Dye

Coomassie

Lambda Max

663 to 667nm

Molecular Weight

318.39

373.90

646.366

18.02

0.0

484.19

326.11

466.592

624.384

646.36

373.896

960.793

Merck Index Reference

14,3905

15, 1455

14,6060

15, 6132

15, 9555

14,9792

CAS Registry Number 2

6487-48-5

CAS Registry Number 3

7647-14-5

CAS RN 1 Min Percentage

95.0

CAS RN 3 Max Percentage

0.8

6.5 to 7.1

3 to 4.5

Physical Form

Crystalline Powder

Green crystalline Powder

Solid

Crystal-Powder at 20°C

Fine Crystalline Powder

Molecular Weight

854.025

Name Note

dye content >60%

Pure

Odor

Odorless

Packaging

EcoSafPak

Glass Bottle

Plastic

Packaging

Poly natural

Product Size

100g

Pub Chem CID

24832074

6099

65565

6328534

6450

102183223

104827

9562061

Quantity

100g

120mL

500mL

25g

Quantity

10g

Recommended Storage

Ambient temperatures

RTECS Number

SO5600000

XP2575000

QJ6440000

SP5740000

QJ6475000

SJ7450000

SMILES

CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C(=CC4=CC(=CC(=C4C3=O)N)S(=O)(=O)[O-])S(=O)(=O)[O-])C)NN=C5C(=CC6=CC(=CC(=C6C5=O)N)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]

CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.O.O.O.[Cl-]

[Cl-].[Cl-].[Cl-].[Zn++].CCNC1=CC2=[S+]C3=CC(NCC)=C(C)C=C3N=C2C=C1C

CC1=C(C(=C(C=C1C2(C3=CC=CC=C3S(=O)(=O)O2)C4=CC(=C(C(=C4C)Br)O)C(C)C)C(C)C)O)Br

CC1=C(C=CC(=C1)C2=CC(=C(C=C2)NN=C3C=CC4=C(C3=O)C(=C(C=C4S(=O)(=O)[O-])S(=O)(=O)[O-])N)C)NN=C5C=CC6=C(C5=O)C(=C(C=C6S(=O)(=O)[O-])S(=O)(=O)[O-])N.[Na+].[Na+].[Na+].[Na+]

CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC(=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3C)C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC)C.[Na+]

CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.[Cl-]

CC1=C(C(=C(C=C1C(=C2C=C(C(=O)C(=C2C)Br)C(C)C)C3=CC=CC=C3S(=O)(=O)O)C(C)C)[O-])Br.[Na+]

CC1=CC(=C(C=C1C2(C3=CC=CC=C3S(=O)(=O)O2)C4=CC(=C(C=C4C)O)C(C)C)C(C)C)O

ChEBI

CHEBI:6872

CHEBI:86155

Synonym

Basic Blue 9

Acid Blue 90, Brilliant Blue G, CBB G-250

Bromthymol Blue

BTB

C.I. 52015; Basic Blue 9

Bromthymol Blue, sodium salt

C.I. 23850; Direct Blue 14

C.I. 23860

Thymolsulfonephthalein

Solubility Information

Soluble in water (10mg/ml at 25°C),ethanol (0.6mg/ml),and EGME (20mg/ml).

Soluble in water.

Solubility in water: 40g/L (20°C). Other solubilities: in methanol: 430g/L

Soluble in water,alcohol and chloroform; Insoluble in ether.

Specific Extinction

700 min. (596nm, in 0.01N NaOH)

Visual TransitionInterval

Pass Test

from pH 6.0 (yellow) to pH 7.6 (blue)

Acid range: from pH 1.2 (red) to pH 2.8 (yellow), alkaline range: from pH 8.0 (yellow) to pH 9.6 (blue)

TSCA

Yes

Clarity

Passes test

Decomposition Information

190°C

EC Number

210-640-4

252-169-7

Solubility

Solubility in water: insoluble. Other solubilities: soluble in diluted alkali solutions, soluble in alcohol

Solubility

Solubility in water: slightly soluble. Other solubilities: soluble in alcohol, alkaline solution

Target Molecule

Protein

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30 results for : Blue Stains and Dyes and Indicators : "Pacific Biosciences of California"

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