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Your source for innovative, indispensable lab equipment, lab supplies, and services. View their wide selection of products for scientific research and education.

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Covid-19 Items

Made in USA

Life Science

Proteomics

Protein Chemistry Reagents and Kits

Cell Lysis and Protein Extraction

Enzymes for Cell Lysis

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Color

Off-white

Content AndStorage

Includes
• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution

Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X

Contains:
• 40 -µL DreamTaq Hot Start DNA Polymerase (5 U/-µL)
• 1.25 mL 10X DreamTaq Buffer (includes 20 mM MgCl2)

Store at -5 to -30°C.

G?° 10 x 1.25 mL Phusion Master Mix with HF Buffer (2X) (provides 1.5 mM MgCl2 in final reaction)
G?° 2 x 500 ++L 100% DMSO

Store at -20°C.

G?° 2 x 1.25 mL Phusion Master Mix with HF Buffer (2X) (provides 1.5 mM MgCl2 in final reaction)
G?° 1 x 500 ++L 100% DMSO

Store at -20°C.

Maxima H Minus Reverse Transcriptase (10000 Units at 200 U/µL concentration) is supplied with 5X RT Buffer (250 mM Tris-HCl (pH 8.3 at 25-°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT).
Store at -20°C.

Store at -20°C.

Contains:
• 20 x 1.25 mL DreamTaq Hot Start Green PCR Master Mix (2X)
• 20 x 1.25 mL Nuclease-free water

Store at -5 to -30°C.

Maxima H Minus Reverse Transcriptase (2000 Units at 200 U/µL concentration) is supplied with 5X RT Buffer (250 mM Tris-HCl (pH 8.3 at 25-°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT).
Store at -20°C.

• 1 x 1 mL Phusion Flash Master Mix

Store at -20°C.

Phusion GC Buffer Pack includes:
• 5X Phusion GC Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Store at -20°C.

Contains:
• 100 -µL DreamTaq Hot Start DNA Polymerase (5 U/-µL)
• 2 x 1.25 mL 10X DreamTaq Buffer (includes 20 mM MgCl2)

Store at -5 to -30°C.

Includes:
• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion GC Buffer
• 5X Phusion HF Buffer
• dNTP mix (10 mM each)
• MgCl2 solution (50 mM)
• Control template (λ DNA)
• 1.3 kb primers (4 µm eac

Upon receipt, store at -20°C in a nonfrost-free freezer. Product shipped with ice.

Store at -20°C

Contains:
• 20 x 1.25 mL DreamTaq Green PCR Master Mix (2X), which includes DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
• 20 x 1.25 mL Nuclease-free water

Store at -20-°C.

Maxima H Minus Reverse Transcriptase (provided as 4 x 10000 Units at 200 U/µL concentration) is supplied with 5X RT Buffer (250 mM Tris-HCl (pH 8.3 at 25-°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT).
Store at -20°C.

Store below -20°C.

Content And Storage

Uracil-DNA glycolase (1 U/µL) and 10X Reaction Buffer. Store at -20°C.

Phusion HF Buffer Pack (F-518L) includes:
• 5X Phusion HF Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO

Store at -20°C.

G?° 10 x 1.25 mL Phusion Master Mix with GC Buffer (2X) (provides 1.5 mM MgCl2 in final reaction)
G?° 2 x 500 ++L 100% DMSO

Store at -20°C.

• 1 x 1 mL Phusion Flash Master Mix

Store at -20°C.

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
Both Phusion HF and GC Buffers provide 1.5 mM MgCl2 in the final

Concentration

100 mM

1U/µL

CompatibleBuffer

10x FastDigest Buffer/FastDigest Green Buffer

10x Buffer Tango

Color

White

Enzyme

Ssp I

Phosphatase

Methyltransferase

SfaAI

DNA Ligase

Dpn I

DNase

SgsI

Uracil-DNA Glycosylase

EcoR I, Bgl II, Nde I, Sal I, Xba I, Kpn I, Eco32I, Xho I, Not I, BamH I, Hind III, Sma I, Pst I

RNase

FidelityvsTaq

52 X

25 X

Format

Off-white lyophilized solid

Powder

Fidelityvs Taq

1 X

For Use With Application

Lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific Antigen

Form

liquid

Format

Crystallized, Lyophilized

GCRichPCRPerformance

Medium

High

Low

GCRich PCRPerformance

High

Description

PhusionG?° GC Buffer Pack

PhusionG?° HF Buffer Pack

Form

Lyophilized

Hot Start

Built-In Hot Start

Labelor Dye

Unconjugated

Overhang

3'-A

Blunt

Quantity

4 x 10000U

500 x 50uL Rxns

500U (2U/-µL)

100 reactions

100U (2U/-µL)

200 x 50-µL Rxns (500 x 20-µL Rxns)

10000U

2.5-µg (250U)

2000U

1000U

100 x 50uL Rxns

10++g (1000U)

500 U

1000 rxns

Quantity

6mL

5 x 200U

100U

10g

200 u

200U

0.5mL

100mg (>=50U/mg)

4 x 2,500U

Shipping Condition

Approved for shipment on Wet or Dry Ice

Includes

With High Fidelity buffer

With High-Fidelity buffer

With GC Buffer

Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS

2 x 1mL of 5X Reaction Buffer

Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration.

Inhibitors

Surface-active reagents such as Dodecyl Sulfate, Alcohols and Fatty Acids, Imidazole and Indole derivatives are inhibitors via formation of change-transfer complexes

MethylationSensitivity

Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive

Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive

Molecular Weight

14388

Optimal Reaction Temperature

50-55° C

Product Line

FastDigest

Maxima

EpiJET

DreamTaq

RiboLock

Phusion

Product Type

Ribonuclease A

Lysozyme, Chicken Egg White

Lysozyme

Label Type

Unlabeled

Reaction Format

SuperMix or Master Mix

Ribonuclease HActivity

None

ReactionFormat

Separate Components

Standalone

SuperMix or Master Mix

Size FinalProduct

20 kb or less

Polymerase

phi29 DNA Polymerase

Phusion Hot Start II DNA Polymerase

Phusion High-Fidelity DNA Polymerase

T4 DNA Polymerase

DreamTaq DNA Polymerase

Phusion Flash II DNA Polymerase

OptimalReactionTemperature

37° C

p H

Optimal ReactionTemperature

50-55° C

SensitivetoHeatInactivation

Yes

Reaction Speed

Fast

Reagent Type

Enzyme for Cell Lysis

OptimalReaction Temperature

37° C

ReactionSpeed

Fast

Standard

Reverse Transcriptase

Maxima H Minus

Source

Bovine pancreas

Chicken Egg

TypeIISRe

Storage Requirements

0°C

-20°C

Source

Chicken Egg White

If you are viewing this page as a nonregistered user, the price(s) displayed is list price. To view your customer-specific pricing, log in or become a registered user here.

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FEREM0821

45 results for : Enzymes for Cell Lysis : "Fisher Scientific"

Contact the GSS Team Today!
GSS-Sales@govsci.com | 800.248.8030 | www.govsci.com

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