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Cytiva, now with the life sciences business from Pall Corporation, is a global biotechnology leader dedicated to helping customers discover and commercialize the next generation of therapeutics. Cytiva brings dedicated technical expertise and a broad portfolio of tools, technologies, and services that enable the development, manufacture, and delivery of transformative medicines to patients.

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Covid-19 Items

Made in USA

Life Science

Microbiology

Molecular Biology Reagents and Kits

Nucleic Acid Introduction Reagents

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Additives

With: Porcine trypsin, HBSS, EDTA Without: Calcium magnesium

Application

Purification of proteins and peptides in microliter scale

Suitable for use in ECL, western blotting applications. ELISA Immunocytochemistry

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

For the rapid purification of DNA for a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labeled nucleotides from DNA labeling reactions

Application_4

Other

Average Particle Size

700 nm diameter

400 nm diameter

Application_19

High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation.

Brand

Life Sciences

Certificate of Analysis

Yes

Chemical Stability

The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules

Color

colorless

Column Capacity

< 1 mg/mL

Dimensions_1

132 mm (diameter)

Dimensions_29

132 mm (diameter)

Dimensions_33

132 mm (diameter)

Estimated Shelf Life from Manufacture Date

24 months

12 months

For Use With

Fraction collector F9-T

Biacore 8K+, Biacore 8K, Biacore S200, Biacore T200 and Biacore 4000 SPR systems

Biacore 8K+, Biacore 8K, Biacore T200, Biacore S200, and Biacore 4000 SPR systems

The kit is designed for Biacore 8K+ and Biacore 8K SPR systems.

Biacore SPR systems (except for Biacore 4000). The volume supplied in Biotin CAPture Reagent limits use with Biacore 4000, and methodology support for using the reagent with this system is not available.

Fraction collector F9-R

Flow Rate

0.01 to 0.50 mL/min

Field245

Plasmid confers resistance to 100 Î¼g/ml ampicillin

Format

Liquid

Format

Circle

Format_6

Buffer

Circle

Guidance

The kit contains 1 x Piston 1 x Seal 1 x Spring *The wetted parts in the piston kit are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100 % Ethyl acetate, 100 % Hexane and 100 % Tetrahydrofura

Includes

10x reaction buffer: 200 mM Tris-HCl, pH 8.0, 100 mM MgCl2; Dilution buffer: 50 mM Tris-HCl, pH 8.0

Indicator

Pottassium Iodide

Phenolpthalein

Inhibitor

Pefabloc TH benzamidine, AEBSF, APMSF, antithrombin III, Antipain, Î±1-antitrypsin, aprotinin, chymostatin, hirudin, leupeptin, PMSF

Input Volume

100 Âµl solution or up to 900 mg agarose

Kit Contents

Ready-To-Go GenomiPhi HY cakes, 3 Ã— 8-well strips; 2x Denaturation buffer, 1 Ã— 2.6 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps

Ready-To-Go GenomiPhi V3 cakes, 3 Ã— 8-well strips; 2x Denaturation buffer, 1 Ã— 1.2 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps

Sample Buffer (white cap) 5 Ã— 0.5 ml; Reaction Buffer (Blue cap) 5 Ã— 0.5 ml; Enzyme mix (yellow cap) 5 Ã— 20 Âµl; Positive control DNA (pUC19, 2 ng/Âµl) 1 Ã— 50 Âµl

Sample Buffer (white cap) 1 Ã— 0.5 ml; Reaction Buffer (Blue cap) 1 Ã— 0.5 ml; Enzyme mix (yellow cap) 1 Ã— 20 Âµl; Positive control DNA (pUC19, 2 ng/Âµl) 1 Ã— 50 Âµl

Sample Buffer 1 x 225 Âµl; Reaction Buffer 1 x 225 Âµl; Enzyme Mix 1 x 25 Âµl; Control DNA (Lambda), 10 ng/Âµl 1 x 20 Âµl

Series S Sensor Chip CAP, Biotin CAPture Reagent, Regeneration Stock 1, Regeneration Stock 2

TempliPhi premix 1 x 20 ml; Denature buffer 10 mM Tris-HCl, pH 8.2, 0.5 mM EDTA, 1 x 20 ml; Positive control pUC19 DNA 1 x 10 Î¼l, 2 Î¼g/ml

BIAnormalizing solution (70%), 90 mL

Ready-To-Go GenomiPhi V3 cakes, 5 Ã— 96-well plate; 2x Denaturation buffer, 5 Ã— 1.2 ml; Control DNA (Lambda), 500 ng; 5 x 12 Strips of 8 domed caps

BIAtest solution, with HBS-EP, 65 mL
BIAnormalizing solution (70%), 90 mL
BIAdesorb solution 1, 2 Ã— 500 mL
BIAdesorb solution 2, 2 Ã— 500 mL
Series S Maintenance Chip
Note: Due to the Biocidal Products Regulation, the BIAdisinfectant solut

Biotin CAPture Reagent, 50 Î¼g/mL in HBS-EP buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% Surfactant P20), 3.4 mL.

Sample Buffer 1 x 0.9 ml; Reaction Buffer 1 x 0.9 ml; Enzyme Mix 1 x 100 Âµl; Control DNA (Lambda), 10 ng/Âµl 1 x 20 Âµl

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Material

Ethylene propylene diene (EPDM) Aluminium oxide (Al oxide)

Nitrocellulose (NC)

Ligand_7

Protein A

Order code

SH30236.02

SV30031.01

SH30042.01

Order Information

Custom options of the product, including larger pack sizes, are available. Please contact your local sales representative or Technical Support.

Custom options of the product are available. Please contact your local sales representative or Technical Support to request., Larger pack sizes available on request; please inquire.

Order E. coli BL21 separately.

Pack size

100 mg

24 reaction

25 reactions

50 columns

500 reactions

1 pack

1 piece

100 mL

2000 reactions

500 units

90 mL

100 reactions

20 columns

500 mL

1 kit

100 purifications

480 reactions

50 pieces

Parameter

Ab Buffer Kit

Pore Size

0.45

Product Size Avg

> 10 kb

Quantity

Reaction time1

16-18 h

Recognition Site

PreScission Protease

Sample Type

PCR mixtures, enzyme reactions, DNA solutions, agarose gel slices

Sample weight1

1 pgâ€“10 ng of purified plasmid or M13 DNA; bacterial or M13 liquid cultures, colonies, plaques or stocks

Purity

The enzyme is purified to apparent homogeneity and is free of all contaminating endonucleases, exonucleases and ribonucleases

Reaction Time

4-18 h depending on starting material

1.5-2 h

4 h

Sample Size

50 bp-10 kb

Sample Volume

25 to 50 Î¼l

< 100 Î¼l

50 Î¼l

< 1 mL

< 2.5 mL

Sample Weight

10 ng of genomic DNA or non-purified cell lysates

10 ng of genomic DNA; or non-purified cell lysates

1 pgâ€“10 ng of purified plasmid or M13 DNA; bacterial or M13 liquid cultures, colonies, plaques or stocks

Scale

Lab

Select Product

Reel, phenophthalein

Stability_25

The TempliPhi premix is stable at -70Â°C for 6 months from the date of manufacture. The denature buffer and positive control DNA are stable at -70Â°C or -15Â°C to -30Â°C for 12 months from the date of manufacture

Storage Conditions

2Â°C to 8Â°C

Store all products at +2 Â°C to 8 Â°C except BIAdesorb solution 1, which should be stored at room temperature (SDS precipitates at low temperature).

The product can be stored for 2 months or less at -20Â°C. For storage longer than 2 months, store at -70Â°C Thaw components on ice and maintain at 0â€“4Â°C during handling. DO NOT warm above 4Â°C prior to amplification

-20Â°C

4â€“8Â°C, 20% Ethanol

Store at +2 Â°C to 8 Â°C

Store Denature buffer and control DNA at -70Â°C or -15Â°C to -30Â°C. Store TempliPhi premix at -70Â°C.Do not store TempliPhi premix at -15Â°C to -30Â°C. Thaw the premix on ice and maintain at 0â€“4Â°C during handling. Do not allow premix to warm above 4Â°

Store the kit at ambient temperature. Once opened, completely reseal the pouch containing the Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended. Store recons

2 â€“ 8Â°C, except Regeneration Stock 1, which may be stored at room temperature to avoid precipitatoin.

Store the kit at -70Â°C. The enzyme mix must be stored at -70Â°C; all other components may be stored at -20Â°C. Thaw components on ice and maintain at 0Â°C to 4Â°C during handling.

Sterilization

0.1 Âµm sterile filtered

Storage

4Â°C, TE buffer (10 mM Tris/HCl, 1mM EDTA, pH 7.6)

Store frozen

4 to 30Â°C

Storage conditions

4–8°C, 20% Ethanol

Storage Temperature

Room Temperature

Supplied As

Lyophilized powder

Type

Reel

Vector

pGEX-4T-1, pGEX-4T-2, pGEX-4T-3, pGEX-2TK

Wetted Part

yes

Unit Definition

One unit of enzyme cleaves >= 90% of 100 Î¼g of a test GST fusion protein when incubated in 1 Ã— PBS at 22Â°C for 16 h.

Yield

PCR 10 Âµl elution volume-65%, PCR 50 Âµl elution volume-82%, 300 mg agarose 10 Âµl elution volume-57%, 300 mg agarose 50 Âµl elution volume-91%

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Sera-Mag SpeedBead Streptavidin-Blocked 3 µm Magnetic Particles, 1mL

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