GenomiPhi V3 Ready-To-Go DNA Amplification Kit, 24 Reactions
Representative amplification of the whole genome.
Preformulated, predispensed, singledose, lyophilized cakes for rapid reaction setup and greater reproducibility.
Quick and simple automationfriendly protocol; no thermal cycler required.
Shipping and storage at room temperature provides easy access and reduced carbon footprint.
Yields highquality DNA (e.g. for highthroughput genotyping and DNA archival).
illustra ReadyToGo GenomiPhi HY DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from GE Healthcare. It contains all of the components necessary for whole genome amplification by isothermal strand displacement amplification. The reaction mixture is provided as predispensed, singledose, lyophilized cakes in either strips of 8 tubes, or 96well plates.
Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity. The average product length is over 1NA kb.
The illustra ReadyToGo GenomiPhi HY DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 4NA to 6NA µg DNA can be achieved in four hours from only 1NA ng of genomic DNA input. The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual.
GenomiPhi amplified DNA is suitable for various applications such as genotyping, cloning, sequencing, and DNA archival.