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Cytiva, now with the life sciences business from Pall Corporation, is a global biotechnology leader dedicated to helping customers discover and commercialize the next generation of therapeutics. Cytiva brings dedicated technical expertise and a broad portfolio of tools, technologies, and services that enable the development, manufacture, and delivery of transformative medicines to patients.

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dNTPs

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Filtering Funnels

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Nonsterile Syringe and Syringeless Filters

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Ion Chromatography IC Columns

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Freezers

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Proteinogenic Amino Acids

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Life Science Water

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Protein Affinity Purification Reagents and Kits

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Qualitative Filter Paper

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Brooms and mops and brushes and accessories

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Goods Buffers

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Ion Chromatography (IC) Autosamplers and Injectors

Liquid Chromatograph (LC) Autosamplers and Injectors

Sponges

Tubes for Blood Collection

Additional Assays
Gamma GlobulinAlkaline PhosphataseLactate DehydrogenaseSGPTSGOTpHAlbuminBlood Urea NitrogenCreatinineTotal ProteinTotal BilirubinSodiumPotassiumCalciumChlorideInorganic PhosphorusOsmolalityTIBCPercent SaturationGlucoseIgG
Lactate DehydrogenaseSGPTSGOTpHTotal ProteinAlbuminBlood Urea NitrogenCreatinineTotal BilirubinSodiumPotassiumCalciumChlorideInorganic PhosphorusOsmolalityIronTIBCPercent SaturationGlucoseIgGGamma Globulin
Gamma GlobulinAlkaline PhosphataseLactate DehydrogenaseSGPTSGOTpHAlbuminBlood Urea NitrogenCreatinineTotal BilirubinSodiumPotassiumCalciumChlorideInorganic PhosphorusIronTIBCPercent SaturationGlucoseIgG
Gamma GlobulinAlkaline PhosphataseLactate DehydrogenaseSGPTSGOTpHTotal ProteinAlbuminBlood Urea NitrogenCreatinineTotal BilirubinSodiumPotassiumCalciumChlorideInorganic PhosphorusOsmolalityIronTIBCIgG
Progesterone Magnesium Insulin Selenium IgG Cholesterol Potassium HDL Calcium LDL Chloride Triglycerides Copper Gamma Globulin Glucose Phospholipids Osmolality Alkaline Phosphatase Iron Lactate Dehydrogenase TIBC SGPT Percent Saturation SGOT B1 B2 B6 Niac
Progesterone Sodium Testosterone Potassium Cortisol Calcium Corticosterone Chloride FSH Inorganic Phosphorus LH Glucose Insulin Albumin T3 Blood Urea Nitrogen T4 Creatinine TSH Total Bilirubin Calcitonin 17 Beta Estradiol Estrone Alkaline Phosphatase Lact
With: L-glutamine
Gamma GlobulinAlkaline PhosphataseLactate DehydrogenaseSGPTSGOTpHTotal ProteinAlbuminBlood Urea NitrogenCreatinineTotal BilirubinSodiumPotassiumCalciumChlorideInorganic PhosphorusOsmolalityIronTIBCPercent Iron SaturationGlucoseIgG
InsulinGamma GlobulinAlkaline PhosphataseLactate DehydrogenaseSGPTSGOTpHAlbuminBlood Urea NitrogenCreatinineTotal BilirubinSodiumPotassiumCalciumChlorideInorganic PhosphorusIronTIBCPercent SaturationGlucoseIgG
ProgesteroneInsulinCholesterolHDLLDLTriglyceridesGamma GlobulinPhospholipidsAlkaline PhosphataseLactate DehydrogenaseSGPTSGOTB1B2B6NiacinBiotinRetinolCaroteneTocopherolB12FolateVitamin C1,25-dihydroxy vitamin DpHTotal ProteinAlbuminBlood Urea NitrogenCrea
Additives
With: HEPES Without: L-glutamine
With: L-glutamine Without: Sodium bicarbonate
Without: Calcium, magnesium
With: L-glutamine, EBSS
With: Porcine trypsin, HBSS, EDTA Without: Calcium magnesium
Without: L-glutamine
Animal Origin
Bovine, Porcine
Application
Designed for gravimetric analysis and the preparation of samples for instrumental analysis. For very fine precipitates. Slow filter paper with highest efficiency for collecting very small particles. Also used for many analytical routine methods in differe
Designed to simplify small-scale purification and screening of antibodies Purified antibody for research studies
For screening of selectivity, binding and elution conditions, as well as small scale purifications
High density culture of human immune cells
Designed for gravimetric analysis and the preparation of samples for instrumental analysis. For medium fine precipitates offering medium filtration speed. Applied in a variety of routine methods in quantitative analysis, e.g., determination of the sand co
Epifluorescence microscopy Environmental analysis Cell biology EPA testing Fuel testing Bioassays Parasitology Air analysis Water microbiology
Routine HPLC/UHPLC analysis. Composite assays. Content uniformity. Protein precipitation. Solubility testing. Dissolution testing. Sample filtration. Use with any compound that requires protection from light, such as catecholamines or vitamins.
For labelling via amine groups. CyDyes are suitable for use in applications such as fluorescence resonance energy transfer (FRET), time resolved- fluorescence resonance energy transfer (TR-FRET), and fluorescence polarization (FP)
Smoke stack gas monitoring. Soxhlet extraction. Analyzing pesticide residues. Determining oil/fat content of foods. Analysis of oil and grease in solid wastes.
The membrane is particularly effective in applications requiring higher flow rates and larger volume filtration including clarification or sterilization of aqueous solutions, particulate analysis and removal, air monitoring and microbial analysis. Other a
Industrial applications: analytical chemistry, research
Used as a protective paper for filter press cloths, as well as in processing of cocoa butter and edible oils.
Culture of human immune cells
Sample preparation Microbiological studies Filtration of aqueous solutions
Used for a wide variety of analytical routine applications in different industries like food testing or removal of carbon dioxide and turbidity from beverages.
Used for many routine analytical applications in different industries (e.g., particle separation from food extracts or filtration of solids from digested environmental samples, e.g., for ICP/AAS analysis).
Validation is recommended for specific applications such as Monoclonal antibodies, Anchorage-dependent cells, Virus production, Vaccine production, Insect cell/baculovirus
Used for a wide variety of analytical routine applications in different industries like food testing (e.g., determination of fat content) or removal of carbon dioxide and turbidity from beverages (e.g., beer analysis).
Used in sample preparation, e.g., in the beverage industry for residual sugar determination, acidic spectra, refractometric analysis, and HPLC.
Used in water pollution applications. Used for removing protein from difficult-to-filter beers. Used for determination of chlorophyll and phytoplankton residues. Used for the determination of filterable substances and the residue on ignition (dry weight).
Western, Southern and Northern Blotting
For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules
For microbiological control
Suitable for biological and clinical analysis, sterility tests, and scintillation measurements
Used as a membrane prefilter in applications such as the determination of crop protection agent residues by GC or HPLC, in cold sludge determination of beer, in soot separation before gas analyzers and as roll filters in automatic air filtration units. Hi
Used for high-throughput screening (HTS) applications
Western blot
Western blotting
Application_4
Chromatography system
Cell isolation
Chromatography system|Manual
Cell expansion
Application_5
Chromatography system|Manual
Basis Weight
140
68
70
75
80
155
85
88
44
84
Bed Dimensions
7.7 x 100 mm
16 x 25 mm
26 x 600 mm
16 x 100 mm
16 x 600 mm
7 x 25 mm
Bed Height
100 mm
25 mm
600 mm
Bed Support
Filter
Net
Bed Volume
5 mL
1 mL
5
1
20
320
4.7
Binding Capacity
115-125 µg IgG/cm² (incubation method) 11-43 µg BSA/cm² (incubation method)
150-176 µg IgG/cm² (incubation method) 20-72 µg BSA/cm² (incubation method)
173-203 µg IgG/cm²
162-180 µg IgG/cm²
Capture modes
SNOW detection mode, auto with pre-capture, manual, and time series
Capacity
Up to 100 mL
Capacity Max.
0.4
Bead type
Mag Sepharose Magnetic Beads
Binder
Inorganic binder
Binder Type
Organic
Inorganic
Brand
Life Sciences
Binding Capacity/Column
Approx. 40 mg histidine-tagged protein/ml medium
CAS
64-17-5
BioProcess resin
yes
Certifications/Compliance_16
USDA Regulations
Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities
Cellbag Bioreactor Components and Materials
Film EVA/LDPE fluid contact surface provides biological compatibility; external layers provide strength and low gas permeability Barbed ports in“ Polyethylene Luer connections - Polypropylene MCP connectors in“ Polycarbonate MCX connectors - Polycarbonate
Chemical Stability
Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl
Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.
Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons
Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to acetone, ketones, chlorinated hydrocarbons, aliphatic esters, phenol, NaOH, >10% HCl, >5% acetic acid, or strong mineral acid|å¯èinå—æ¶²ç›¸å±‚æžä½¿ç”¨çš,æ°´æin
Certificate of Analysis
Yes
Chemical Stability (Column Hardware)
Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.
Certifications/Compliance
Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities
Chromatography technique
Hydrophobic Interaction Chromatography
Multimodal ion exchange chromatography
Ion Exchange Chromatography
Antibody affinity chromatography
Group specific affinity
GST-tagged protein purification
Affinity coupling
Histidine-tagged protein purification
COLOR
Green
Red
Blue
Black
White
Color Code
Green
Red
Dark brown
Light brown
White
Column i.d.
10 mm
16 mm
7.7
50 mm
7
16
26 mm
Color
colorless
Translucent
Column Capacity
< 1 mg/mL
Diameter
100 mm
270 mm
30
320 mm
47 mm
50
50 mm
125 mm
13 mm
185 mm
47
90 mm
110 mm
240 mm
30 mm
150 mm
500 mm
Description name
Defined Fetal Bovine Serum, US Origin
Standard Fetal Bovine Serum, US Origin
Characterized Fetal Bovine Serum, Canadian Origin
FetalClone II
Dimensions
25 mm
13 mm
150mmx4m
200x200mm
26 x 34 mm
47 mm
50 mm
200mmx4m
80x90mm
Equivalent in size to 12 x 32 mm vials
H x W x D: 660 x 860 x 710
150 mm
Dimensions_1
300x600mm
47 mm
70 mm
80x90mm
Ã˜ 25 mm
Ã˜ 50 mm
150mmx4m
200mmx4m
200x200mm
300mmx4m
Ã˜ 90 mm
Ã˜ 110 mm
Ã˜ 42.5 mm
Ã˜ 47 mm
100x100mm
36 mm
9.0 cm
Ã˜ 150 mm
Ã˜ 55 mm
Dimensions_29
300mmx4m
300x600mm
47 mm
Ã˜ 50 mm
100x100mm
36 mm
9.0 cm
Ã˜ 150 mm
Ã˜ 42.5 mm
Ã˜ 55 mm
Ã˜ 47 mm
150mmx4m
200mmx4m
200x200mm
70 mm
80x90mm
Ã˜ 110 mm
Ã˜ 25 mm
Ã˜ 90 mm
Dimensions_33
300mmx4m
Ã˜ 50 mm
150mmx4m
200mmx4m
200x200mm
300x600mm
47 mm
70 mm
80x90mm
Ã˜ 110 mm
Ã˜ 25 mm
Ã˜ 90 mm
Ã˜ 150 mm
100x100mm
36 mm
9.0 cm
Ã˜ 42.5 mm
Ã˜ 47 mm
Ã˜ 55 mm
Detection
Fluorescence laser scanners and CCD imagers
DIGE approved
y
Endotoxin Concentration
< 0.005 EU/mL
â‰¤ 1.0 EU/mL
â‰¤ 25 EU/mL
â‰¤ 0.05 EU/mL
â‰¤ 10 EU/mL
For Information Only
Estimated Shelf Life from Manufacture Date
24 months
3 years
See outer packaging
2 years
12 months
18 months
6 months
Features and Benefits 3
Supports use in commercial-scale production, with a range of formats, good alkaline stability, high DBC, and regulatory support file.
Field of Application
Bulk bacterial removal (General) Sediment analysis (Water microbiology and analysis)
Filling Volume
540
Filtration Area
5.7
1.3
20.4
4.2
Filtration Media
Polytetrafluoroethylene (PTFE)
Regenerated Cellulose (RC)
Cellulose Acetate (CA)
Polyvinylidene Difluoride (PVDF)
Filtration Speed
Medium
Slow
Filtration Speed (Herzberg)
Approx. 70
Approx. 77.5
30
300 ml
375
50
Approx. 50
70
Approx. 25
Approx. 140
Approx. 375
Approx. 55
Approx. 60
Approx. 80
Filtration Time to Herzberg
140
Flow Direction
Flow should enter from the inlet
If there is a prefilter, it is located on the inlet side and flow should follow arrows.
Supported bidirectionally. Certain applications may require orientation, i.e., vents. Reverse flow only for low pressure applications.
Bidirectional
Flow Rate
< 1 ml/min|< 1 ml/min
< 20 ml/min|< 20 ml/min
2 ml/min - 10 ml/min|2 ml/min - 10 ml/min
< 20 mL/min
< DarcyÂ´s Law
130 mm/30 min
< 4 mL/min
< 4 ml/min|< 4 ml/min
< 5 ml/min|< 5 ml/min
Flow Velocity
< 450 cm/h|< 450 cm/h
< 500 cm/h|< 500 cm/h
< 600 cm/h|< 600 cm/h
< 150 cm/h|< 150 cm/h
< 300 cm/h|< 300 cm/h
<1800 cm/h
10 cm/h - 50 cm/h
15 cm/h|15 cm/h
< 150 cm/h
For Use With
AKTA avant 150
Biacore 8K+, Biacore 8K, Biacore T200, Biacore S200, and Biacore 4000 SPR systems
Biacore X100, Biacore 3000, and Biacore C SPR systems|Biacore X100ãinBiacore 3000 å’Œ Biacore C ç³»ç»Ÿ
For Use With AKTA start
Dye
Cy5
Endotoxin
Daily sample release requires < 0.125 EU/ml endotoxin
Features and Benefits 2
Specific Fc region interaction enables removal of product-related impurities from asymmetric bispecific antibodies (bsAbs) with protein A binding knocked out on one Fc region.
Field245
Plasmid confers resistance to 100 Î¼g/ml ampicillin
Format_6
Prepacked columns
Circles
Liquid
Prepacked column
Bulk resin
Circle
Roll
Folded
Sheets
For Membrane Butler
Yes
No
Features and Benefits 1
Allows elution at pH ~5, reducing acid-induced aggregation and loss of target antibody.
Field14
50Â°-65Â°
Filtrate Vessel Material
Polycarbonate (PC)
Filter Support
Sieve (frit as accessory)
For use with_31
AKTA go protein purification system
Format
Folded
Roll
Sheets
Circle
Circles
Liquid
Frequency
50/60
Format
Powder
Gel Dimension
130 x 3 x 0.5 mm
70 x 3 x 0.5 mm
180 x 3 x 0.5 mm
240 x 3 x 0.5 mm
Grade
230
Characterized
Defined
Grade 0858 Â½
Grade 1
Grade 91
Grade GF 6
Grade GF 92
Grade GF/A
Grade Shark Skin
8
Grade 589/2
Grade 595
Grade 597 Â½
Grade 598 Â½
Grade 602 h
Grade 604
Grade GMF 150
Standard
1
3MM Chr
41
603 g
934-AH RTU
Grade 4
Grade 50
Grade 520b
Grade 597 Optima
Grade 602 h Â½
Grade QM-A
Specialty filtered
201
Grade 1573 Â½
Grade 595 Â½
Grade 597
Grade GF/C
Grid Color
White
Plain
White/black
Black
Guidance
Strip length: 13 cm Instruction supplied with the gel
Carrier for high resolution isoelectric focusing (IEF)
Strip length: 18 cm Instruction supplied with the gel
Strip length: 24 cm Instruction supplied with the gel
Supplied with the strip holder: 6 x Strip holder cover 1 x 60 ml Strip holder cleaning solution Instructions
*The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100 % Ethyl acetate, 100 % Hexane and 100 % Tetrahydrofuran.
Instructions and specification supplied with the buffer
Storage: Room temperature, maximum 20Â°C, without any contact with heat and radioactive sources. The film can also be stored at 4Â°C
Strip length: 11 cm Instruction supplied with the gel
Strip length: 7 cm Instruction supplied with the gel
Supplied with the strip holder: 1 x Strip holder cover 1 x 60 ml Strip holder cleaning solution Instructions
Heat Inactivate
No
Yes
Height
629 mm
400 mm
Grade
Grade 1 Chr
603
Grade 589/3
GB003
Grade 934-AH
Grade GF 10
Grade_8
Grade 589/2
Grade 589/1
Hemoglobin Concentration
For Information Only
â‰¤ 10 mg/dL
â‰¤ 20 mg/dL
â‰¤ 25 mg/dL
Hold-up Volume of Membrane
<20 Î¼l
<5 Î¼l
<10 Î¼l
Grade Type
Standard
Wet Strengthened
Folded (Prepleated)
Grid Dimension
3.1 mm
plain
3.1
Green Items
No
Housing
Polycarbonate (PC)
Polypropylene (PP)
Pigment free polypropylene (PP)
Humidity
20% to 70% (no dew condensation)
Hydrophilic
Yes
Hydrophobic
No
Yes
Image Output
Gray scale 16-bit .TIF, color Image .JPG, gray scale .JPG
IgG levels
Typically less than 2 Î¼g/mL
Includes
Instructions supplied with the concentractors
1 sensor chip
Installation instructions and port positioning label
3 sensor chips
Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.
Connectors for high- and low-pressure chromatography equipment and instructions.
Injections per vial/well
Max. 9
Inlet Connector
Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing
Female luer lock
Female national pipe thread (FNPT)
Inlet id
0.75 mm (0.029 in)
1 mm (0.04 in)
1.0 mm (0.04 in)
1.55 mm (0.06 in)
Inner Packaging
N/A
Single film bag
Ion Exchanger Type
Strong anion exchanger
Weak cation
Strong cation exchanger
Weak anion exchanger
Ionic Capacity
4-5 mmol/g
3-4 mmol/g
In-line Reference Subtraction
Automatic
Labelling Chemistries
Amine groups
Inner Diameter
22 mm
33 mm
1 mm (0.039 in)
25 mm
26 mm
28 mm
30 mm
10 mm
Length
123 mm
1.5 m
140 mm
60 mm
80 MM
2 m
94 mm
100 mm
90 mm
Irradiated With Lid
No
Licensing
For licensing information, see the Licensing Statements page in the About Us section.
For licensing information, see the Licensing Statements page in the About Us section.
Lifetime
1 ns
<0.3 ns
Ligand
Butyl
Phenyl
Quaternary amine
Alkali-stabilized Protein A-derived (E. coli)
Diethylaminoethyl
Glutathione
Carboxymethyl
Sulfonate group
Sulphopropyl
IMAC immobilized with Ni
Native Protein A
Protein G
Ligand Coupling Method
Cyanogen bromide activation
Epoxy activation
long, hydrophilic spacer arms
Dextran surface extenders
Ether linkages and hydrophilic spacer arm
Includes
N/A
Interface
12.1-inch high-resolution built-in touch screen
Input Voltage
100 to 240 V
Material
Borosilicate glass
Cellulose Acetate
Clear Polystyrene (PS)
Glass Microfiber
Mixed Cellulose Ester (MCE)
Natural Polypropylene (PP)
Polyamide (PA)
Polypropylene (PP)
Absorbent paper and laminated polyethylene
Cellulose
Cellulose Nitrate
Fluoro rubber
Nitrocellulose
Polytetrafluoroethylene (PTFE)
PVDF
Silicone
Stainless steel
High-quality cotton linters
Polycarbonate (PC)
Borosilicate glass fibers
Ethylene propylene diene (EPDM) Polyetheretherketone (PEEK)
Ethylenetetrafluoroethylene (ETFE)
High-purity quartz (SiOâ‚‚)
Nitrocellulose (NC)
Perfluor rubber (PFR) Polyetheretherketone (PEEK) Zirconia Ruby
Polyester (PE)
Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)
Polyetheretherketone (PEEK)
Polyvinylidene Difluoride (PVDF)
PTFE
Reinforced Nitrocellulose (NC)
Interleaving Paper
No
Yes
Matrix
Cross-linked agarose, 4%, spherical
Cross-linked agarose, 6%
Highly cross-linked agarose, 6%
Agarose, 4%
cross-linked agarose, 6%, spherical
Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.
Cross-linked dextran
Polystyrene/divinylbenzene
Composite of cross-linked agarose and dextran
Highly cross-linked agarose
Highly cross-linked agarose with dextran surface extender
Highly cross-linked agarose, spherical
Max power
250
Maximum pressure over packed bed
4.0 MPa (40 bar, 580 psi)
Licensing_22
For licensing information, see the Licensing Statements page in the About Us section.
Kit Contents
Room-temperature stable beads containing ~ 2.5 units of recombinant PuReTaq DNA polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction buffer. When a bead is reconstituted to a 25 Î¼L final volume, the concentration of each dNTP is 200 Î¼M in10
Ligand
Recombinant Protein A (E.coli)
Ligand_7
Protein L
Protein A
Protein A, alkali stabilized
Protein G
Max recommended temperature
800 Â°C
550 Â°C
Membrane Type
ME 26
ME 28
OE 67
TE 38
ME 25
OE 66
ME25
RC55
RC58
ME 24
ME 27
Plain
Matrix
Highly cross-linked 4% agarose
Paramagnetic, spherical, highly cross-linked agarose particles
6% Cross-Linked Agarose
High-flow agarose
Max shelf life
36 months
Method
PP method
Metal Ion Capacity
~15 Âµmol Ni²⁺/ml medium
Multi-Step Purification
Yes, (optional)
Molar Extinction Coefficient
150000 M^-1cm^-1
Nominal Air Flow Rate
2.6 s/100 ml/inÂ²
4 s/100 ml/inÂ²
6.7 s/100 ml/inÂ²
4.3 s/100 ml/inÂ²
6 s/100 ml/inÂ²
13 s/100 ml/inÂ²
3.7 s/100 ml/inÂ²
6.3 s/100 ml/inÂ²
Nominal Ash Content
0.01%
0.02%
0.06%
Nominal Basis Weight
100
87
92
64
65
75
95
53
85
96
Nominal Thickness
190
210
115
435
180
200
205
215
260
475
420
675
Operating Flow Rate, Max
1.0 mL/min
2.6 mL/min
Operating Flow Rate, Recommended
0.5 mL/min
1.3 mL/min
Order Information
Additional pack size available on request, please inquire.
BioProcess resin. Process-scale quantities are available.
Use 20-40 mM imidazole in sample and binding buffers for highest purity.
This product is produced based on a customer order. Delivery time may be longer than for standard products.
Custom options of the product, including larger pack sizes, are available. Please contact your local sales representative or Technical Support.
Caps are not required for Biacore 4000
Custom formulation, testing, packaging, and labeling are available.
Custom options of the product are available. Please contact your local sales representative or Technical Support to request., Larger pack sizes available on request; please inquire.
Order E. coli BL21 separately.
Outlet Connector
Mini-tip
Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing
Male luer
Male slip luer
Outlet id
0.75 mm (0.029 in)
1.0 mm (0.04 in)
Mycoplasma
No
Yes
Pack size
1 mg
100 g
100 pieces
100 reactions
1000 mL bottle
1000 pieces
150 pieces
20 columns
25 Âµmol
25 nmol
25 pieces
4 Ã— 96-well filter plates
400 pieces
5 L
5 mL
50 mL
500 mL
0.1 mg
1 Ã— 1 mL
1 piece
10 L
10 pieces
100 mL
20 mL
250
300 mL
4.7 mL
5 Ã— 5 mL
5 mg
6 x 100 mL
1 Ã— 4.7 mL
1 Ã— 5 mL
1 mL
10 mL
100 mmol
100 sheets
150 mL
2 x 1 mL
200 mL
25 mL
50 columns
50 L
50 sheets
500 g
6 Ã— 500 mL
6 x 500 mL
1 kit
1 L
100/PK
1000 mL
2 Ã— 5 L
4
5 Ã— 1 mL
50 pack
50 pieces
500 pieces
6 x 1000 mL
750 mL
Pack Size
10
25
6
1
1000
2
5
50
Paragraph 2 Heading
Effective purification for antibodies sensitive to low pH
Paragraph 4 Heading
High DBC benefits process economy
Particle Retention in Liquid Max
10
20
< 2
12
25
7
Particle size, d_50V
~15 Âµm
~40 Î¼m
~90 Âµm
~90 Î¼m
~ 9 Âµm
~75 Âµm
~85 Âµm
~ 8.6 Âµm
~34 Âµm
~50 Âµm
pH
7.0 to 7.2
7.0 to 7.4
5.0 to 7.0
pH Range
11-Mar
7-Apr
11-Jun
5.5â€“7.5
10-Mar
pH Stability Short Term
1(<1 day exposure)â€“14(<1 day exposure)
1â€“14
pH stability, CIP
2â€“10
2â€“13
2â€“13.7
3â€“12
3â€“14
1â€“14
2â€“14
2â€“12
4â€“9
pH stability, operational
2â€“10
2â€“13
4â€“12
2â€“12
3â€“12
3â€“9
3â€“10
2â€“9
3 to 12
3â€“11
3â€“13
4â€“13
4â€“9
Physical State
Liquid
liquidSuspension
Solid
Pore Size
1.2
1.2 Âµm
5
5 Âµm
0.02 Âµm
0.1
0.2 Âµm
0.45
0.8 Âµm
0.2
0.45 Âµm
0.8
1 Âµm
Porosity
7 Î¼m
10 Î¼m
10 or 80 Âµm
20 Î¼m
Porosity Min
66%
74%
Power Consumption
8 VA
800 VA
Approx. 0.3 kVA
Prefilter
No
Prefilter Material
100% Borosilicate Glass Fiber GMF 150 10 Âµm: 1 Âµm GF/F 0.7 Âµm
Pressure Limit
20 bar (2 MPa, 290 psi)
2 MPa
Pressure/Flow Specification
400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height
Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Non-Pyrogenic
non-reactive
Operating Pressure Max
2 bar - forward direction (29 psi - forward direction)
5.2 bar (75 psi)
40 bar (4 MPa) (580 psi)
6.9 bar (100 psi)
4.1 bar (60 psi)
Operating System
Microsoft Windows 7 Professional SP1 (x32 or x64 bit) (Note: Computer and OS is not included with the purchase of UNICORN)
Operating Temp Max
100Â°C
135Â°C
130Â°C
35Â°C
80ÂºC
130
40Â°C
Operating Temp Min
4Â°C
Operating Temperature
<180 Â°C
18Â°C to 28Â°C
<500 Â°C
4Â°C-35Â°C
4Â°C-40Â°C
Number of Ports
7
Paragraph 3 Heading
Specific interaction for the Fc region
Paragraph 5 Heading
Alkaline stability for robust resin cleaning-in-place (CIP)
Particle Size
45 Âµm-165 Âµm
45 Î¼m-165 Î¼m
24 Âµm-44 Âµm
25 Âµm-75 Âµm
Origin
U.S.
Canada
USDA
pH Gradient
Linear
Nonlinear
Paragraph 3 Body Text
MabSelect™ mild elution resin has a specific interaction with the Fc region of human IgGs. The resin can separate product-related impurities in the capture step for asymmetric bsAbs in which only one of the Fc chains includes the classical protein A-binding Fc-motif.
Paragraph 5 Body Text
MabSelect™ mild elution resin can maintain its DBC over many cycles, as well as withstand harsh cleaning conditions to reduce risk of bioburden incidents and carryover between batches. In a CIP lifetime study with repeated loading of mAb cell culture supernatant and cleaning with 0.25 M NaOH, the relative remaining DBC after 100 purification cycles was 82%. Even when cleaned with 0.5 M NaOH, the resin can be used for 100 cycles with more than 80% remaining DBC (shown in an accelerated alkaline stability study).
Porosity Max
84%
77%
Power
100 to 240 V, ~ 50 to 60 Hz
pH ligand fully charged
Entire pH range
pH Stability Long Term
43843
Processing
Heat Inactivated
None
Physical Form
Spray-dried powder
Number Of Wells
96
Operating Humidity
20% to 70%, RH (no dew condensation)
Operating Temp
Room temperature
Outer Diameter
1.58 mm (1/16 in)
Pressure/flow characteristics
Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).
Paragraph 1 Body Text
Avoid acid-induced aggregation in pH sensitive antibodies with MabSelect™ mild elution protein A resin. Lower aggregation means better yields and process economy in the capture step.
Paragraph 2 Body Text
Typical elution pH values in the protein A capture step are in the 3.5-4 range. However, some antibody-derived molecules aggregate at this low pH—including bsAbs (containing an Fc region), IgG4, and Fc fusion proteins. Aggregation can compromise monomeric purity and yield and can place additional pressure on subsequent polishing steps. MabSelect™ mild elution resin is part of Cytiva’s toolbox of resins designed to solve a wide range of purification challenges and reduce the risk of unnecessary disqualification of molecules due to insufficient purification.
Paragraph 4 Body Text
High dynamic binding capacity (DBC) allows high mass throughput of processed antibody per resin volume unit. It also benefits productivity and process economy. At 6 min RT, the DBC for MabSelect™ mild elution resin is 60 g/L using trastuzumab. This is lower than the DBC of MabSelect PrismA™ resin (72 g/L), but for pH-sensitive molecules, higher elution pH may be more important than DBC.
Partical Size Dry
40 Âµm-120 Âµm
Product Size Avg
> 10 kb
Properties
Fast
Fast, smooth
Medium fast, grained
Medium fast, thin
Medium to slow, wet strength, thin, creped
Solution
Very fast, wet strength, extra thick
Creped
Medium
Medium fast, thick
Slow, dense
Medium fast
Slow-medium
pH Stability Cleaning-in-Place (CIP)
1 to 14
Protein Adsorption
Low
Protein Binding
Medium
Purity
> 99% triphosphate
â‰¥ 98% triphosphate
Pump Type
Piston pump, metering type
Protection Class
IP 21
Quantity
1
Recommended Flow Rate
5 ml/min
1 ml/min
Quantum Yield
>0.15
Resin Type
Capto
Sepharose Fast Flow
MabSelect
Sepharose High Performance
Sample Volume
<= 5 ml
<= 13 ml
4 to 50 ÂµL
Sample Weight
10 ng of genomic DNA or non-purified cell lysates
Scale
Lab
Lab|Process
Lab|Process development|Process
Lab|Process development
Safety Certifications
2006/42/EC (MD), 2004/108/EC (EMC), 1999/5/EC (R&TTE)
Sample Volume Min
1 ml
Sealing
Heat-fused
Shelf Life
12 Months
24 months
18 Months
36 months
Size
500 mL
100 ml
Relative Humidity
20%-95% (non condensing)
Reaction Time
1.5-2 h
Reagent type
Fluorescent Labeling Reagents
Solubility
Insoluble in cold and hot water
It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be
Soluble in cold water and hot water
Ribbon Filter Color
White
Solvent Resistance
Medium
Specialty Type
Dialyzed
Charcoal/Dextran filtered
Stability
Stable
Sterilization
0.2 Âµm sterile filtered
Autoclave at 121ÂºC (131ÂºC max)
Autoclave at 121ÂºC (131ÂºC max) at 1 bar (15 psi) for 20 minutes
Certain filter devices have been sterilized. Capsule may be autoclaved at 121Â°C for 20 minutes. However, an integrity test should be performed after autoclaving.
0.1 Âµm sterile filtered
Non-sterile
0.1 Âµm sterile filtered
Autoclave at 121ÂºC
Can be autoclaved at 121Â°C for 20 minutes (maximum 132Â°C). Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed
May be autoclaved at 121Â°C for 20 minutes. Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed after autoclavin
Sterilization Method
Filtered through sequential 100 nm pore-size rated filters; Gamma Irradiated.
Filtered using sequential 100 nm pore-size rated filters.
Filtered through sequential 40 nm pore-size rated filters.
Filtered through three sequential 100 nm pore-size rated filters.
Filtered through sequential 100 nm pore-size rated filters.
Storage
15Â° to 30Â° C
2 to 8Â°C, 20% Ethanol
2 C -8 C
2Â° to 30Â° C
4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate
4Â°C to 30Â°C, 20% Ethanol
2Â° to 8Â°C, away from light
2Â°C to 8Â°C in 20% ethanol
Sera should be stored at -10Â°C or lower. Once thawed, sera should be stored at 2Â° to 8Â°C.
15Â° to 30Â°C
2Â° to 8Â° C
2Â° to 8Â°C, away from light and moisture
4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH
4Â°C to 8Â°C
Store frozen
4 to 30Â°C
4 to 30Â°C, 0.2 M sodium acetate in 20% Ethanol
4 to 30Â°C, 20% Ethanol
4 to 8Â°C, 20% Ethanol
Storage Conditions
2 - 8Â°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20Â°C in aliquots to avoid repeat freeze thaw cycles.
-20Â°C
4 - 8Â°C, 20% Ethanol, 10% medium slurry
4ËšC to 30ËšC, 20% ethanol
-15 to -30Â°C
2 - 8Â°C
2 â€“ 8Â°C
2Â°C to 8Â°C, protected from light
4â€“30Â°C
Ambient temperature in pouch with desiccant
Store the kit at ambient temperature. Once opened, completely reseal the pouch containing the Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended. Store recons
-15 to -30Â°C, Avoid light, store in the dark.
2â€“8Â°C
4 - 30Â°C
STERILITY
Non-sterile
Sterile
Sterility_13
Nonsterile
Sterile
Storage Temperature
Freezer: - 20 C
Room Temperature
Support System
Polypropylene (PP)
Stability
Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Sterile
No
Yes
Sterility
Nonsterile
Storage Requirements
Ambient
Supporting Analysis
Qualitative
System
Sepax C-Pro|Sefia|Sepax 2
Xuri
Systems
Xuri
Tested for
BluetongueBovine AdenovirusBovine ParvovirusBovine RSVBVDVCytopathogenic AgentsHemadsorbing AgentsRabies VirusReovirus
Bluetongue Bovine Adenovirus Bovine Parvovirus Bovine RSV BVDV Cytopathogenic Agents Hemadsorbing Agents Rabies Virus Reovirus
Theoretical Plates
5000 m-1
Type
Hardened low ash
Reel
Roll
3-Piece
Integral Comparison Strip
Ashless
Typical Particle Retention in Liquid
10 Âµm
11 Âµm
1.5 Âµm
25 Âµm
1.6 Âµm
2.7 Âµm
1.2 Âµm
1.5 Î¼m
2.5 Âµm
8 Âµm
Typical Thickness
150
320
550
160
170
180
350
190
Typical Trace Element Levels
Aluminium 2.5 Âµg/g Antimony <0.5 Âµg/g Arsenic <0.5 Âµg/g Barium <0.5 Âµg/g Boron <1 Âµg/g Calcium 8.3 Âµg/g Chromium 1.5 Âµg/g Copper 2 Âµg/g Iron 12 Âµg/g Lead <0.5 Âµg/g Magnesium 4 Âµg/g Manganese <0.5 Âµg/g Mercury <0.5 Âµg/g Potassium 2.3 Âµg/g Sil
Aluminium 3.6 Âµg/g Antimony <0.5 Âµg/g Arsenic <0.5 Âµg/g Barium <0.5 Âµg/g Boron <1 Âµg/g Calcium 27.5 Âµg/g Chromium 1 Âµg/g Copper 0.9 Âµg/g Iron 13.7 Âµg/g Lead <0.5 Âµg/g Magnesium 21 Âµg/g Manganese <0.5 Âµg/g Mercury <0.5 Âµg/g Potassium 6.2 Âµg/g
Typical water flow rate
10 ml/min
105 ml/min
247 ml/min
341 ml/min
5 ml/min
57 ml/min
143 ml/min
274 ml/min
USDA Safety Testing Included
Yes
USDA Inspected
TYPE
Sheets
Volume_28
100-500 Âµl
400 Âµl - 2 ml
2-6 ml
5-20 ml
Wall Thickness
1
2 mm
1 mm
1.5
Weight Max
5.5 mg/cmÂ²
5.0 mg/cmÂ²
Weight Min
4.3 mg/cmÂ²
3.6 mg/cmÂ²
Well Bottom
Filter
Wetted Materials
Polyetheretherketone (PEEK)
Ethylenetetra fluoroethylene (ETFE)
Polyetheretherketone (PEEK), polytetrafluoroethene (PTFE)
Polyetheretherketone (PEEK)/Polytetrafluoroethene (PTFE)
Silicone
Wetted Part
yes
no
Wall Thickness Type
Single
Double
Weight
84 g/mÂ²
Vent
On inlet
Volume
400 Âµl - 2 ml
5-20 ml
100-500 Âµl
2-6 ml
Typical Pressure Drop Over the Packed Bed
~ 3 MPa
Wetted materials1
Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)
With Pad
No
Viscosity Max
15
Voltage
AC100â€”240V~, Voltage variationÂ±10%
Web Page Header
MabSelect™ mild elution protein A resin
Width
900

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