Government Scientific Source

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$0~$10(0)

$10~$25(0)

$25~$50(7)

$50~$75(24)

$75~$100(34)

$100~$200(90)

$200~$300(78)

$300~$400(51)

$400~$500(35)

$500~$750(58)

$750~$1000(38)

$1000~$5000(60)

$5000~$10000(6)

$10000~$50000(8)

$50000~$100000(0)

$100000~$3000000(0)

Absorbance

0.04 max. at 210nm

Anhydrousor Hydrate

Anhydrous

Antibiotic ResistanceBacterial

Ampicillin (AmpR)

Additional Information

Vapor Pressure: 97mbar at 20°C

Brand

Life Sciences

Axygen®

CAS Registry Number 1

75-05-8

9007-49-2

Average Particle Size

400 nm diameter

700 nm diameter

Boiling Point

81°C

Application_19

High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation.

Assay Percent Range

>=99.9 %

BacterialorYeastStrain

Not Included

Chemical Name or Material

Glycogen

Acetonitrile

ChEBI

CHEBI:38472

Chem AlertStorageSymbol

Red

Certificate of Analysis

Yes

CAS_NO

57-55-6

Color

Colorless

Green

Description

Collect, stabilize, process, transport, and archive blood samples with Whatman Non-Indicating FTA Classic Cards.

MagJET Separation Rack, 12 x 1.5mL tubes

Bacterial/Yeast Strain: DH10B

Chemical Substance ID

24848147

Cloning Method

Restriction Enzyme ⁄ MCS, Blunt PCR

Concentration

4mg/mL

Density

0.781g/cm-?

Content AndStorage

• Lysis Solution, 40 mL
• Precipitation Solution (10X), 8 mL
• 1.2 M sodium chloride, 10 mL

Store at room temperature.

Cleanup Symbol

Solvent

For Use With Application

Clean sample cuts from FTA/FTA Elute cards

Cloning (Blunt PCR, Restriction Enzyme G?° MCS)

Purifies linear and circular DNA from 200 to 50,000bp

Efficient removal of unincorporated dNTPs, salts and Enzymes

Forensics.Transgenic identification.Transfusion medicine/HLA typing.Plasmid screening.Food and agriculture testing.Drug discovery.Genomics.STR analysis.Animal identification.Whole genome amplificationMolecular biology.

Isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples

PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription

Rapid and efficient purification of DNA and Oligonucleotides

Automated genomic DNA extraction from whole blood samples collected in EDTA, ACD or heparin tubes

Break hard/brittle samples

Isolate genomic DNA from whole blood, cultured cells, yeast, mouse tissue or numerous other cell sources

Sample Preparation; Sample Lysis; Nucleic Acid Isolation & Purification; Molecular Biology; Gene Expression; Microbiological Analysis; Gene Function; Gene Regulation

Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR

Isolation of DNA from white Blood cells, tissue culture cells, animal tissue, plant tissue, yeast and gram positive and gram negative bacteria

Ideal for applications requiring multiple paramagnetic isolations of biomolecules

Country of Origin

DOT Information

DOT Class 3, : Flammable Liquid

EC Number

200-338-0

For Use With Equipment

FastPrep-24; FastPrep 120; FastPrep-96; SuperFastPrep-1

Magnetic Stand

Wizard genomic DNA purification kit

Wizard SV Gel and PCR cleanup system

Direct STR amplification

Harris Micro Punch

Maxwell 16 and 16 MDx instruments

MagneSphere and PolyATtract system components

Format

Spin or Vacuum

Powder

Four sample areas

Tube(s)

96 and 384-well plate

Formula Weight

41.05

Decomposition Information

>500°C

Form

liquid

Export control classification number

EAR99

Estimated Shelf Life from Manufacture Date

24 months

Formula

Service & Support

CH₃CH(OH)CH₂OH

Fluorescence Background

Pass Test

Grade

DNA Synthesis

HAZ

Hazardous

Yes

Health Hazard 2

DANGER!

Highthroughput Compatibility

Not High-throughput Compatible (Manual)

Compatible (Vacuum manifold)

Health Hazard 3

Emergency Overview
Flammable liquid and vapor. Irritating to eyes. May cause skin and respiratory tract irritation. May cause central nervous system effects. Liver and kidney injuries may occur. Use personal protective equipment. Use only unde

Harmonized Code US

3822005090

InChIKey

WEVYAHXRMPXWCK-UHFFFAOYSA-N

Includes

Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution

50 x 2mL Tubes Lysing Matrix E, 60mL Sodium Phosphate Buffer, 8mL MT Buffer, 25mL PPS Solution, 66mL Binding Matrix, 50 spin modules, 50 catch tubes, 12mL concentrated SEWS-M, 20mL DES, 200µL BBS Gel loading dye, user manual

[other]

48 Maxwell 16 MCD LEV cartridges, 50 LEV purification plungers, 50 Elution tubes, 20mL Lysis Buffer, two 1mL Proteinase K solution, 20mL Elution Buffer

100mL Cell lysis solution, 50mL Nuclei lysis solution, 25mL Protein precipitation solution, 50mL DNA rehydration solution, 250µL RNase solution

Input Amount

1-1000 cells or 1fg-10ng of input DNA

MDL Number

MFCD00064272

MFCD00166026

items per unit of measure

1 kit

1 each

Kit Contents

Sample Buffer 1 x 225 Âµl; Reaction Buffer 1 x 225 Âµl; Enzyme Mix 1 x 25 Âµl; Control DNA (Lambda), 10 ng/Âµl 1 x 20 Âµl

TempliPhi premix 1 x 20 ml; Denature buffer 10 mM Tris-HCl, pH 8.2, 0.5 mM EDTA, 1 x 20 ml; Positive control pUC19 DNA 1 x 10 Î¼l, 2 Î¼g/ml

Sample Buffer 1 x 0.9 ml; Reaction Buffer 1 x 0.9 ml; Enzyme Mix 1 x 100 Âµl; Control DNA (Lambda), 10 ng/Âµl 1 x 20 Âµl

Sample Buffer 10 x 2.25 ml; Reaction Buffer 10 x 2.25 ml; Enzyme Mix 10 x 250 Î¼l; Control DNA (Lambda) 10 ng/Î¼l 10 x 20 Î¼l

Sample Buffer (white cap) 5 Ã— 0.5 ml; Reaction Buffer (Blue cap) 5 Ã— 0.5 ml; Enzyme mix (yellow cap) 5 Ã— 20 Âµl; Positive control DNA (pUC19, 2 ng/Âµl) 1 Ã— 50 Âµl

Single Cell GenomiPhi Reaction buffer 1 x 275 Âµl, Single Cell GenomiPhi Enzyme mix 1 x 25 Âµl, Single Cell GenomiPhi Amplification mix 1 x 25 Âµl, Single Cell GenomiPhi DTT Solution 1 x 100 Âµl, Single Cell GenomiPhi Lysis buffer 1 x 100 Âµl and Single C

Sample Buffer (white cap) 1 Ã— 0.5 ml; Reaction Buffer (Blue cap) 1 Ã— 0.5 ml; Enzyme mix (yellow cap) 1 Ã— 20 Âµl; Positive control DNA (pUC19, 2 ng/Âµl) 1 Ã— 50 Âµl

Molecular Weight

76.09

0.0

41.053

PAC code

7280

7132

Pack size

500 reactions

1000 reactions

100 reactions

2000 reactions

25 reactions

Packaging

Glass Bottle

Product Family

Axygen® AxyPrep MAG PCR Clean-up Kit

Product Gross weight

0.2

0.01

Product Line

CloneJET

MagJET

GeneJET

Product Type

LEV Blood DNA Kit

System

FTA Classic Card, 4 sample areas per card

Genomic DNA

NAP-5

Lysing Matrix A (garnet)

Solution

NAP-10

RNase A Solution, 4mg/mL

Wizard DNA Cleanup System

Kit

NAP-25

Accessory

2-Position

Genomic DNA Purification Kit

PurificationTarget

Genomic DNA

Plasmid DNA

Radioactive

FALSE

Reaction time1

16-18 h

SampleType

Bacteria

Liquid Samples (e.g. Serum), Plant Samples, Cells, Tissue, Yeast, Bacteria, Blood

Bacteria, Yeast, Tissue, Cells, Buccal Samples

Size

250microl

30 reactions

500microl

50microg

100 isolations x 300microl

100u

1,000u

100microg

25/pack

250 preps

250microg

50mL

50microl

1 each

1ml

2 x 96 preps

4 x 96 preps

1 x 96 preps

12 x 75mm

125ml

150 reactions

48 preps

5 entry and 20 transfer reactions

500 reactions

90microg

100 reactions

200 preps

50 preps

500 isolations x 300microl

25 preps

100 preps

25ml

32.5microg

500u

5microg

Titratable Base

0.0006meq/g max.

Viscosity

0.36 cP at 20-°C

Item Class Description

Consumables

Reagents

Includes

100mL Wizard™ DNA cleanup resin, 100 Wizard™ minicolumns, 100 3cc Syringe barrels

L CMSGradientSuitability

Pass Test

No of Reactions

250 Preps

48 preps

50 preps

Melting Point

-46°C

Pub Chem CID

6342

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Quantity

10mg

Each

250 preps

1mL

50 Pack

50 Preps

100 preps

12 x 75mm

200g

48 Preps

100 Pack

Storage Conditions

Store the kit at -70Â°C. The enzyme mix must be stored at -70Â°C; all other components may be stored at -20Â°C. Thaw components on ice and maintain at 0Â°C to 4Â°C during handling.

Store the kit at -70ÂºC. The enzyme mix must be stored at -70ÂºC; all other components may be stored at -20Â°C.

Store Denature buffer and control DNA at -70Â°C or -15Â°C to -30Â°C. Store TempliPhi premix at -70Â°C.Do not store TempliPhi premix at -15Â°C to -30Â°C. Thaw the premix on ice and maintain at 0â€“4Â°C during handling. Do not allow premix to warm above 4Â°

The product can be stored for 2 months or less at -20Â°C. For storage longer than 2 months, store at -70Â°C Thaw components on ice and maintain at 0â€“4Â°C during handling. DO NOT warm above 4Â°C prior to amplification

Molecular Formula

C2H3N

Qty/Pk

1 / Pk

Recommended Storage

Keep cold

Sample weight1

1 pgâ€“10 ng of purified plasmid or M13 DNA; bacterial or M13 liquid cultures, colonies, plaques or stocks

Size

1.5mL

PAC Code Description

MFT GX & XT Reagent

MFT GX & XT Consumables

SMILES

CC#N

IUPAC Name

acetonitrile

Species

Human

Stability

This product has been designed to deliver high quality results for up to 12 months from the manufacturing date. Please refer to the expiration date on the product label.

Stability_25

The TempliPhi premix is stable at -70Â°C for 6 months from the date of manufacture. The denature buffer and positive control DNA are stable at -70Â°C or -15Â°C to -30Â°C for 12 months from the date of manufacture

Storage Temperature

Room Temperature

Reaction Time

2 to 4 hours

4 h

1.5-2 h

4-18 h depending on starting material

Refrigerated/Frozen

Refrigerated

Frozen

Sample Weight

10 ng of genomic DNA or non-purified cell lysates

1 pgâ€“10 ng of purified plasmid or M13 DNA; bacterial or M13 liquid cultures, colonies, plaques or stocks

Order Information

Custom options of the product are available. Please contact your local sales representative or Technical Support to request., Larger pack sizes available on request; please inquire.

Custom options of the product are available. Please contact your local sales representative or Technical Support to request. Larger pack sizes available on request.

Physical Form

Liquid

Powder

Product Size Avg

>10kb

> 10 kb

Storage Requirements

Dry Ice (-80°C)

Room temperature

15 to 30°C

+15C to +30C

UoM Description

each

Vector

pJET1.2/blunt

One Unit Contains

1 Each

1 Kit

Percent Purity

>=99.9%

No of Samples

Prep Scale

< 100 µg (Small-Scale) Plasmid DNA

Product Gross Weight Desc

UNSPSC Code

41105331

41106001

Vapor Pressure

97mbar at 20-°C

Water

10ppm max.

Synonym

methane, cyano

1/4 in. Zirconium Oxide Banded Satellite for Grinding

Qty/CS

1 / Cs

Quantity

5 mL

20 reactions

40 reactions

20mL

Refractive Index

1.3405 to 1.3425 (at 25°C)

Sample Size

125µL per sample area

300µL

Residueafter Evaporation

1.0ppm max.

Yield

5 to 15µg/300µL

UN Number

1648

Volume Metric Sample

125 mL

Titratable Acid

0.008mEq/g max.

UoM

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