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Chromatography

Chromatography Consumables

Reagents and Buffers

Chromatography Media

$0~$10(0)
$10~$25(0)
$25~$50(182)
$50~$75(52)
$75~$100(17)
$100~$200(73)
$200~$300(53)
$300~$400(21)
$400~$500(27)
$500~$750(40)
$750~$1000(29)
$1000~$5000(99)
$5000~$10000(8)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

Absorbance

0.02 AU max. at 330nm

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

For purification of DNA from small molecules by gel filtration

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

Application_4

Chromatography system

Cell isolation

Chromatography system|Manual

Autoignition Temp

800 °F

~800 °F

Bed Volume

5 mL

1 mL

320

Assay

=99%

99.8%

Application_5

Chromatography system|Manual

Bed Height

25 mm

600 mm

30 mm

100 mm

Capacity

1.7 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

0.8 meq/mL by wetted bed volume

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

4.7 meq/g by dry weight

=1.1 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

Average sample loading capacity

400 mg

50 mg

Bed Dimensions

16 x 100 mm

7 x 25 mm

16 x 25 mm

26 x 600 mm

16 x 30 mm

10 x 100 mm

6.4 x 100 mm

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

Bead type

Mag Sepharose Magnetic Beads

Assay Percent Range

99.8% min by GC

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

At least 10 mg histidine-tagged protein/ml sedimented medium.

BioProcess resin

yes

Boiling Point

189°C

2230 C

CAS Registry Number 1

67-68-5

Applications

ion chromatography: suitable

Binding Capacity/ml Chromatography Medium

At least 10 mg histidine-tagged protein

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

> 25 mg horse liver GST/mL resin

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

CAS_NO

1344-28-1

11128-95-3

69011-19-4

126850-14-4

69011-20-7

9004-54-0

9003-70-7

9041-35-4

9050-97-9

112945-52-5

39389-20-3

39339-85-0

63181-98-6

65546-95-4

112926-00-8

1343-88-0

104219-63-8

60177-39-1

62610-50-8

65099-79-8

69011-15-0

7631-86-9

Chemical Stability

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Stable in all commonly used buffer systems

Commonly used aqueous buffers

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Stable in all commonly used aqueous buffers

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Chemical Substance ID

24854187

329756032

24863799

24865203

24852837

24854823

329759181

329759183

329759193

329759187

329768201

329768868

24857354

24867159

24854191

24854645

24857358

329752001

329763462

329752189

24854194

24857352

329763461

329759188

24852120

24854190

ChEBI

CHEBI:28262

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Stable in commonly used aqueous buffers, 1 M NaOH

Chem AlertStorageSymbol

Gray

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

COLOR

Blue

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Chromatography technique

Antibody affinity chromatography.

Group specific affinity

Ion Exchange Chromatography

Antibody affinity chromatography

GST-tagged protein purification

Hydrophobic Interaction Chromatography

Histidine-tagged protein purification

Affinity coupling

Density Max.

1.078 g/ml

1.2 g/ml

1.135 g/ml|

Color

Colorless to slightly yellow

White

Gold/Brown

Colorless

Column i.d.

6.4

26 mm

16 mm

Chemical Name or Material

Dimethylsulfoxide

Concentration

1 N in NaOH (Regenerant concentration)

Decomposition Information

>190°C

Column volume

1 mL

8 mL

Composition

nitrogen content, =1.0%

Density

1.100g/cmÂ³

Flow Rate

< 20 mL/min

< DarcyÂ´s Law

Recommended: 0.5 to 2.0 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 5 ml/min

2 ml/min - 10 ml/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

>10 ml/min

>60 ml/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

< 20 mL/min |< 20 mL/min

1-4ml/min

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 ml/min

> 4 ml/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

Flow Velocity

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

‰¤ 600 cm/h|‰¤ 600 cm/h

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

< 150 cm/h

<15 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 450 cm/h

< 450 cm/h|< 450 cm/h

< 500 cm/h

30-150cm/h

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

Exclusion Limit Small Organics

5 x 10Â³

Estimated Shelf Life from Manufacture Date

8 years

5 years

3 years

Field14

50Â°-65Â°

EC Number

215-681-1

238-878-4

215-691-6

231-545-4

Endotoxin Activity Max.

2 EU/ml

0.12 EU/mg

< 0.12 EU/ml

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

Exclusion Limit Peptides

4 x 10Â³

Format_6

Bulk resin

Prepacked column

Prepacked columns

Empirical Formula

O2Si

MgO3Si

Form

powder or crystals

powder

fine powder

beads

fine crystals

Formula

O2Si

Service & Support

MgO₃Si

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

SiO₂

(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃

Al2O3

Flashpoint (°C)

> 240 C

Formula Weight

78.13

100.39

60.08

Feature

ferrule type packed column

Flash Point

87°C (188.6°F)

Format

matrix material

Grade

for column chromatography

high-purity grade

high-purity grade (Davisil Grade 62)

high-purity grade (Davisil Grade 923)

high-purity grade (Davisil Grade 643)

HPLC

Standard

high-purity grade (Davisil Grade 646)

high-purity grade (7754)

high-purity grade (Davisil Grade 633)

certified reference material

high-purity grade (Davisil Grade 636)

nuclear grade

technical grade

H-Codes

H372

H319

H373

H371

Intrinsic Viscosity

~ 17

IOD-Codes

Ion Exchanger Type

Strong anion exchanger

Weak anion

Weak anion exchanger

Weak and strong anion

Multimodal weak cation exchanger

Strong cation exchanger

Weak cation exchanger

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand

Cibacron Blue

Human IgG

Quaternary amine

Calmodulin

Strep-Tactin XT

Sulphopropyl

Carboxymethyl

Alkaline-stabilized protein A-derived (E.coli).

Diethylaminoethyl

Porcine heparin

Recombinant protein A (E.coli)

Native protein A

Alkali-stabilized Protein A-derived (E. coli)

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Ligand Coupling Method

Epoxy activation

Ether linkages and hydrophilic spacer arm

Epoxy

Maleimide activation

Dextran surface extenders

Ether linkages

Cyanogen bromide activation

Triazine

Reductive Amination

Ligand_7

Butyl, octyl, phenyl

Protein L

Protein A

Protein A, alkali stabilized

Protein G

MDL Number

MFCD00011232

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00211881

MFCD00132707

MFCD00132771

MFCD00132712

MFCD00132722

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00132718

MFCD00132726

MFCD00145831

MFCD03096474

MFCD00084428

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00145586

MFCD00145841

MFCD03791384

Melting Point

18.4°C

Health Hazard 2

WARNING!

Operating Flow Rate, Max

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

< 4 mL/min

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

1.0 mL/min

2.6 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

Operating Flow Rate, Recommended

0.5 mL/min

0.50 mL/min

0.04 mL/min

0.075 mL/min

0.80 mL/min

0.75 mL/min

1.3 mL/min

0.30 mL/min

Optical Activity

7.0 (5% in slurry)

~7 (5% in H 2 O, suspension)

6.5 (5% in slurry)

Order Information

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

For research use only.

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

Percoll PLUS is for research use only.

HiLoad columns are prepacked XK columns.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Osmolality

288-310 mOsm/kg

276-298 mOsm/kg

322-344 mOsm/kg

< 30 mOsm/kg

P-CODES

P260

P314

P305 + P351 + P338

Pack size

500 mL

1 L

10 mL

200 mL

25 mL

500 g

6 x 500 mL

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 columns

6 mL

2 x 1 ml 10% slurry

50 mL

1.7 mL

100 mL

125 mL

25 g

1.5 g

5 Ã— 1 mL

6 x 1 L

1 Ã— 4.7 mL

10 g

2 x 1 mL

15 g

100 g

5 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

20 mL

300 mL

Packaging Layout

500 g in poly drum

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

100, 500 g in poly bottle

5, 25 kg in fiber drum

10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles

500 g in poly bottle

2.5 kg in poly drum

1 ft3 in fiber drum

25 kg in fiber drum

25, 500 g in poly bottle

2.5, 5, 25 kg in fiber drum

Particle Size

60 Âµm

24-44 Âµm

60 Î¼m-200 Î¼m

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

Particle size distribution, dry

50 â€“ 150 Âµm

20 â€“ 80 Âµm

Particle Size in Methanol Max

163

Particle size, d_50V

~90 Î¼m

~100 Î¼m

~34 Âµm

~34 Î¼m

~85 Âµm

~90 Âµm

34 Âµm

~ 8.6 Âµm

~50 Âµm

~75 Âµm

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

pH stability, CIP

2â€“13

2â€“13.7

3â€“12

1â€“14

2â€“14

3â€“13

2â€“12

3â€“10

4â€“13

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

2â€“10

3â€“14

Pore Size

0.7-0.85 cm 3 /g pore volume (approx.)

110 Å pore size

60 Å mean pore size

0.30 mL/g pore volume

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

0.5 mL/g pore volume

0.7-0.9 cm 3 /g pore volume

30 Å

~0.55 mL/g pore volume

0.75 cm 3 /g pore volume

150 Å

90 Å mean pore size

~0.8 cm 3 /g pore volume

Pressure/Flow Specification

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

Residueafter Evaporation

5ppm max.

Signal Word

Danger

Warning

Stability

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Stokes Radius

~ 10

Storage

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2-8C

2 to 8Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

2 to 8Â°C, 100% Isopropanol

Identification

Pass Test

Synonym

DMSO

Group To be Coupled

Primary amines

-NHâ‚‚

Theoretical Plates

> 42 000 m^-1

5000 m-1

> 48 000 m^-1

> 13000 m-1

> 43 000 m^-1

Type of ion exchanger

Strong anion exchanger (quaternary ammonium)

Strong cation exchanger (methyl sulfonate)

Includes

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Connectors for high- and low-pressure chromatography equipment and instructions.

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Ionic Capacity

0.18-0.24 mmol Cl^-/ml medium

0.29-0.35 mmol Cl^-/ml medium

0.18-0.25 mmol H⁺/ml medium

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.13-0.17 mmol/ml

0.15-0.20 mmol H⁺/mlÂ

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

Ligand

Recombinant GammaBind G type 3 lacking albumin-binding region

Ligand Density

3 mg GammaBind G, type 3/ml medium

~ 5 mg/mL resin

3 mg Protein A/ml medium

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

Approx. 13 Î¼mol/ml

Linear Formula

MgO3Si

SiO2

(C10H12 · C10H10 · C8H8 · C3H9N)X

(CH₃)₂SO

(C10H12 · C10H10 · C8H8)x

Material

Plastic/Stainless Steel

N/A

Matrix

6% Cross-Linked Agarose

Highly cross-linked agarose with dextran surface extender

Matrix

Composite of cross-linked agarose

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Composite of cross-linked agarose and dextran

Highly cross-linked 4% agarose

Highly cross-linked 6% agarose

agarose, 6%, spherical

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Highly cross-linked agarose, spherical.

cross-linked, 6% agarose particles

Highly cross-linked agarose

Rigid, cross-linked agarose, spherical

Cross-linked agarose, 4%

4% Agarose

4% cross-linked agarose

cross-linked agarose, 4%, spherical

2% agarose

Cross-linked dextran

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Ligand Concentration

4.5-8 mg gelatin/ml drained resin

~25 Âµmol phenyl/mL resin

~45 Âµmol phenyl/mL resin

~4mg heparin/mL resin

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Molecular Weight

0.0

60.08

78.129

101.96

100.39

Operating pH

0 - 7

0 - 14

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Osmolality

< 25 mOsm/kg

Partical Size Dry

20 Âµm-50 Âµm

40 Âµm-120 Âµm

18 Âµm-111 Âµm

50 Âµm-150 Âµm

>100 Âµm

>20 Âµm

pH ligand fully charged

Above 6

Below 9

< 9

Entire pH range

Pictogram

GHS07

GHS08

Particle size distribution, wet

90â€“280 Âµm

15â€“88 Âµm

100â€“300 Âµm

20â€“80 Âµm

55â€“165 Âµm

87â€“510 Âµm

Particle Size in Methanol Min

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Purity

99.8%

>=99%

Quantity

50 g

100 g

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Sample Volume

400 to 1000 Î¼l

<= 13 ml

4 to 50 ÂµL

25 to 500 ÂµL

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

<= 5 ml

100 to 300 Î¼l

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

IUPAC Name

methylsulfinylmethane

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

S-Codes

26-36

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Pack Size

Packaging

Amber Glass

Percent Purity

≥99.9%

pH Range

5.5â€“7.5

8.5â€“9.5

8.9â€“9.9

pH stability, operational

3â€“10

2â€“10

pH 6 to 10

2â€“12

3 to 12

3â€“11

3â€“13

2â€“9

4â€“13

4â€“9

2â€“13

4â€“12

3â€“12

3â€“9

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Liquid

Physical State

liquidSuspension

Liquid

solid

Pressure/flow characteristics

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Storage Conditions

4â€“30Â°C

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

20% ethanol at 4Â°C to 30Â°C

Surface Area

380 m 2 /g

500 m 2 /g

~30 m 2 /g

250-350 m 2 /g

530 m 2 /g

175-225 m 2 /g

~500 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

289 m 2 /g

480 m 2 /g

550 m 2 /g

300 m 2 /g

470-530 m 2 /g

=480 m 2 /g (approx.)

430-530 m 2 /g

Swelling factor

~1.7

Quality

monodispersed

dry

wet

regenerated

Molecular Formula

C2H6OS

Resin Type

MabSelect

Sepharose CL-4B

Capto

Sepharose Fast Flow

Sepharose High Performance

RTECS Number

VV7310000

VV7315000

Synonyms

Dowex(R) 1X8-200 Ion Exchange Resin

XAD16N

Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous

Amberlite(R) IR120 hydrogen form

Silica gel 110 NH2

Activated magnesium silicate; Magnesium silicate

Silica gel

pH Stability Cleaning-in-Place (CIP)

1 to 14

Recommended maximum operational flow velocity

300 cm/h

R-CODES

48/20

36/37/38

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

>1600 C

Recommended Flow Rate

5 ml/min

1 ml/min

Refractive Index

1.4770 to 1.4800 (at 25°C)

Water

0.04% max.

Quantity

500 g

SMILES

CS(=O)C

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

WGK

nwg

PurityGrade Notes

Mobile phase for HPLC applications

Pub Chem CID

679

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Purity GradeNotes

Mobile phase for HPLC applications

Scale

Lab|Process development

Lab

Lab|Process development|Process

Lab|Process

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Vapor Density

>1 (vs air)

<1 (vs air)

Viscosity

1.98 mPa/s at 25Â°C

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

System

Sepax C-Pro|Sefia|Sepax 2

Viscosity Max

Titratable Acid

0.001mEq/g max.

Wetted Part

Well Volume

8.5

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