Government Scientific Source

Promega Corporation is a leader in providing innovative solutions and?technical support?to the life sciences industry. The company’s product portfolio of over 4,000 products support a range of life science work across areas such as cell biology; DNA, RNA and protein analysis; drug development; human identification and molecular diagnostics.

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Enzymes for Cell Lysis

Life Science Buffers

Restriction Enzymes Aa - Bz

$0~$10(0)
$10~$25(5)
$25~$50(15)
$50~$75(25)
$75~$100(67)
$100~$200(102)
$200~$300(75)
$300~$400(80)
$400~$500(46)
$500~$750(83)
$750~$1000(50)
$1000~$5000(106)
$5000~$10000(29)
$10000~$50000(26)
$50000~$100000(2)
$100000~$3000000(5)

Accession Number

NP_005347

Animal Derivative

None

Recombinant from E. Coli

ADP

Bovine serum or albumin

Chicken blood, AMV-RT pellets

Rabbit blood for/or lysate

Blood product, mouse

Bovine derived product

Additional description

Green

Yellow

Application

DNA/RNA Isolation,Viral Nucleic Acids

Beilstein Reference

3916578

Block Description

Four sets of 96 x 0.2 mL tubes or four 96-well plates

Binding Capacity

12 µg Based on specified 15 µL aliquot of supplied magnetic bead suspension = 1.5 mg beads @ 100 mg/mL

Brand

Axygen®

chemagic

Life Sciences

USA Scientific Brand

Business Unit

Cap Material

Polypropylene

Cap Style

Dome

Flat top

No cap

Caps Included

Yes

Auto Restart On Power Failure

Yes

Carton Qty

1000

125

400

Block Uniformity

Block at 55°C: ±0.3°C

Animal Origin

Bovine, Porcine

Bottom Style

Conical

Cap Color

Clear

CAS_NO

146986-50-7

30827-99-7

4294-45-5

57-66-9

3544-24-9

391210-10-9

4673-26-1

856925-71-8

9001-15-4

9073-78-3

91079-46-8

9001-12-1

9001-75-6

9031-72-5

12650-88-3

22816-60-0

26628-22-8

66701-25-5

74299-50-6

8049-47-6

9001-59-6

9013-53-0

144-48-9

20325-40-0

2304-81-6

2922-28-3

57-50-1

9001-45-0

9025-35-8

9031-11-2

9055-20-3

Certifications Compliance

Human DNA, DNase, RNase, PCR inhibitor-free

Certificate of Analysis

Yes

Chemical Substance ID

24278032

24278240

24278206

329747363

24894029

24893736

24898976

57652280

24278411

24278465

24896083

329751672

329773119

Chemical Name or Material

Pyruvate Kinase

S-Acetylthiocholine iodide

Autoclavable

Brand Name

chemagic

Capacity

0.1 mL

0.2 mL

0.5 mL

CAS Registry Number 1

1866-15-5

9001-59-6

9001-75-6

ClosureType

Hinged

Color

Amber

COLOR

Yellow

Clear

White

Green

Natural

Red

Blue

Concentration

>=3,000U/mg

100 mM

50 X

>=20?/mg

>160U/mg

10 mM

10 X

25 mM

Color

Brown

CompatibleBuffer

10x Buffer Tango

10x FastDigest Buffer/FastDigest Green Buffer

Unique Buffer (10x Buffer AarI)

Unique Buffer (10x Buffer SacI)

10x Buffer G

Content And Storage

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl2 solution

Both Phusion Green HF and GC Buffers provide 1.5

Contains:
• 10 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 2 x 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 10 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (suffic

Content AndStorage

Includes:
• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

S

The RevertAid RT Kit contains:
• RevertAid Reverse Transcriptase
• RiboLock RNase

Contains:
• 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 µL reactions.

Contains:
• 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 2 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (sufficient f

G?° 4 x 1.25 mL 2X Phire Hot Start II PCR Master Mix
G?° 1 x 0.5 mL DMSO
G?° 4 x 1.25 mL Water, nuclease free

Store at -20°C.

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 ++M Forward GAPDH Primer, 10 ++M Reverse GAPDH

• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
• 10 mM dNTP Mix

Store at -5 to -30°C.

Contains:
• 200 µL Phire Hot Start II DNA Polymerase
• 5 x 1.25 mL 2X Phire Plant Tissue PCR Buffer (includes dNTPs and MgCl2)
• 40 µL Universal Control Primer mix (25 µm each)
• 2 x 5 mL Dilution Buffer

Store at -2

Contains:
• 1 mL Phusion Blood Direct PCR Master Mix (2X)
• 40 µL Universal Control Primer Mix
• 0.5 mL 50 mM EDTA
• 1.5 mL 50 mM MgCl2
• 0.5 mL DMSO
• 1.25 mL Water, nuclease-free
• 50 µg O'GeneRul

Contains:
• 4 x 1.25 mL PCR Master Mix (2X), which includes:
— Taq DNA polymerase (0.05 U/µL),
— reaction buffer,
— 4 mM MgCl2, and
— 0.4 mM of each dNTP
• 4 x 1.25 mL Nuclease-free water

Store a

Contains:
• 4 x 1.25 mL DreamTaq Hot Start Green PCR Master Mix (2X)
• 4 x 1.25 mL Nuclease-free water

Store at -5 to -30°C.

Contains:
• 4 x 1.25 mL DreamTaq Green PCR Master Mix (2X), which includes DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
• 4 x 1.25 mL Nuclease-free water

Store at -20-°C.

Includes:
• Phusion DNA Polymerase, 2 U/µL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl2

Both Phusion Green HF and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
Both Phusion HF and GC Buffers provide 1.5 mM MgCl2 in the final

Includes:

• 2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration)
• Water, nuclease-free
• DMSO

Store at -20°C.

Country of Origin

Depth Cm

Depth In

DetectionMethod

SYBR

EC Number

232-468-9

232-748-0

232-864-1

232-870-4

247-852-1

277-809-2

205-630-1

220-867-0

222-586-9

232-582-9

243-737-5

200-664-3

232-585-5

200-344-3

232-606-8

232-616-2

232-629-3

232-973-4

235-747-3

293-434-7

Disclaimer

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006)

EINECS Number

232-577-1

217-474-1

DOT Information

Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: TOXIC SOLIDS, ORGANIC, N.O.S.

Enzyme

AarI

BcuI (SpeI)

BglI

Bst1107I

Dpn I

Eco31I

EcoR I

Kpn2I

MunI

BshTI

Eco47III

MreI (Sse232I)

Nde I

Psp1406I

Xba I

Xho I

XmaJI

AdeI

Bgl II

BpiI (BbsI)

Eco147I

Eco52I

Eco81I

Esp3I (BsmBI)

Mph1103I

PagI

Pst I

Pvu I

Pvu II

Sca I

AatII

BpiI

Hind III

Kpn I

KspAI

LguI (SapI)

MauBI

MssI

Nhe I

Not I

PacI

Pfl23II

SacI

Expression System

Baculovirus

Features and Benefits 1

Highest Specificity: Selectively digests dsDNA and DNA in DNA-RNA hybrids; virtually inactive towards ssDNA or RNA.

FidelityvsTaq

2 X

52X

25 X

52 X

Electrical

Universal, 100 to 240V, 50 to 60 Hz

For Use With Application

Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases

For tissue dissociation combined with other Enzymes such as Elastase, Trypsin/Papain

Used to breaks phosphodiester bonds, catalyze the hydrolysis of various Nucleotide polyphosphates

Used to lyse E. coli for preparation of plasmid DNA from transformed cell cultures

Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)

Used as a proteolytic enzyme; used to break down the proteins into their components; i.e.; peptides and aminoacids

Features and Benefits 3

Thermostable: Able to withstand high temperatures without denaturation or loss in activity.

Features and Benefits 2

Premium Purity: RNase-free, protease-free, and animal-free.

Fidelityvs Taq

1 X

For Use With Equipment

7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus System, StepOne System

Form

Liquid

Format

24 Rod Head,Automated

Format

Lyophilized powder

Solid

Crystalline Powder

Tube

Formula

C14H21N3O · 2HCl · H2O

C8H8Cl2N2S • HCl

C₃₆H₇₁NO₃

C₅H₅N₅ · HCl

C12H22O11

C16H14F3IN2O4

Service & Support

Formula Weight

289.18

GCRich PCRPerformance

Medium

High

GCRichPCRPerformance

Medium

Low

High

Gel Compatibility

Acrylamide Gels, Agarose Gels

Functional Testing

Each batch of PuReTaq Ready-To-Go PCR Beads is tested in a real-time PCR assay to ensure its ability to generate a specific PCR product with Î» DNA; and with negative control samples (untemplated reactions) to ensure the lowest possible levels of either e

Gene Alias

LCK

GeneID Entrez

3932

Harmonized Code EU

38221900

Hazardous

Yes

Health Hazard 1

H301+H311-H315-H319-H335

Hamonized Tariff Code/Schedule B Number

3822.19.00.00

Harmonized Code US

3822190080

Heated Lid Pressure

Adjustable

Height In

21.0625

height inch

Height Cm

53.5

Hot Start

Built-In Hot Start

Export control classification number

EAR99

Includes

2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease-free; DMSO

2X Phire Hot Start II PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease free; DMSO

Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL); 5X Phusion Green HF buffer (provides 1.5 mM MgCl2 in the final 1X concentration); 5X Phusion Green GC buffer (provides 1.5 mM MgCl2 in the final 1X concentration); DMSO; 50mM MgCl2 solution

Includes Labelor Dye

Yes

Includes LabelorDye

Yes

Inhibitors

Cysteine, EDTA or o-phenanthroline, alpha2-macroglobulin - a large plasma glycoprotein, alpha2-macroglobulin, alpha1-antitrypsin

Gluctathione, Cysteine and Ascorbic Acid

Kit Contents

Room-temperature stable beads containing ~ 2.5 units of recombinant PuReTaq DNA polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction buffer. When a bead is reconstituted to a 25 Î¼L final volume, the concentration of each dNTP is 200 Î¼M in10

Bulk first-strand cDNA reaction mixes (5)DTT solutionpd(N)₆ primerNot I-d(T)18 bifunctional primermRNA standardRNase-free water

Ready-To-Go GenomiPhi V3 cakes, 1 Ã— 96-well plate; 2x Denaturation buffer, 1 Ã— 1.2 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps

LabelorDye

Unconjugated

Health Hazard 2

GHS H Statement
H301-H312-H315-H319-H335
Toxic if swallowed.
Harmful in contact with skin.
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.

Labelor Dye

Unconjugated

Kinase Family

SRC Kinase Family

Kinase Group

Tyrosine Kinases

Label Type

Unlabeled

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Health Hazard 3

P261-P264b-P270-P271-P280-P301+P310-P302+P352-P304+P340-P305+P351+P338-P312-P330-P332+P313-P361-P363-P501c

Heated Lid Temperature

35 to 115°C, or off

Mass

10 µg

Max Heating Rate

3.4°C per second

MDL Number

MFCD00080335

MFCD00130486

MFCD00130542

MFCD00130629

MFCD00130830

MFCD00131557

MFCD00131731

MFCD00677817

MFCD00038402

MFCD00075782

MFCD00081305

MFCD00130876

MFCD00131789

MFCD00008028

MFCD00012488

MFCD00036749

MFCD02259139

MFCD00081946

MFCD00130623

MFCD00132962

MFCD04040933

MFCD00165549

MFCD00214165

MFCD08460907

MFCD00003536

MFCD00007989

MFCD00080261

MFCD00081840

MFCD00130774

Material

Polypropylene

Memory

1000 programs max

Methylation Sensitivity

Not CpG methylation-sensitive, dcm methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive

Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive

MethylationSensitivity

Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive

Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not dam methylation-sensitive, dcm methylation-sensitive, CpG methylation-sensitive

Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive

Not CpG methylation-sensitive, dcm methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive

Melting Point

205°C to 209°C

MFCD Number

MFCD00011819

Min Temperature C

Molecular Weight

0.0

115000

357.41

184.96

136.15

289.175

292.33

239.69

60.6 kDa

65.01

535.15

565.95

149.10

171.59

285.36

314.55

Molecular Formula

C7H16INOS

Molecular Weight

317.21

No ofReactions

200 reactions

Order Information

Custom options of the product, including larger pack sizes, are available. Please contact your local sales representative or Technical Support.

Output

USB port

Overhang

Blunt

Package Height (in Inches)

1.8

0.2

3.4

Package Length (in Inches)

0.7

1.6

Package Type

125/Bag

1000/Bag

400/Bag

10/Box

Package Width (in Inches)

1.6

0.2

Number Of Blocks

OptimalReaction Temperature

37° C

PAC Code Description

Chemagic Kits MSM I

Package Weight (in Pounds)

0.1

0.2

PCRMethod

qPCR

Physical Form

Powder

Lyophilized Powder

Physical State

Solid

Polymerase

Phire Hot Start II DNA polymerase

Phusion Hot Start II DNA Polymerase

Phusion U Hot Start DNA Polymerase

DreamTaq DNA Polymerase

Phusion Hot Start II High-Fidelity DNA Polymerase

Phusion High-Fidelity DNA Polymerase

Phusion Blood II DNA Polymerase

Power Hz

50/60

Power Vac

100 to 240

Polymerase

Taq DNA Polymerase

Number of Tests

55 reactions

OptimalReactionTemperature

37° C

Product Family

Axygen® PCR Strip Tubes

Axygen® PCR Tubes and Caps

Axygen® PCR Strip Caps

Overhang

3'-A

p H

9.8 to 10.4.5

PAC Code

6814

Product Size Avg

> 10 kb

Pack size

55 reactions

96 reactions

Package Quantity

Packaging

Poly Bottle

Product Type

Phosphodiesterase I

Catalase

Lysozyme

Proteinase K

Collagenase Type 4

T4 DNA Polymerase, LIC-Qualified

Product cubic cm

6564

Product Gross Weight Desc

Product Height (in.)

6.2

Product Line

Phire

RevertAid

DreamTaq

Phusion

FastDigest

Maxima

Percent Purity

98%

Product Type Specs

KOD Hot Start Master Mix

Protein Form

Full Length, Recombinant, Cytoplasmic

Protein Tag

His Tag

Product Width (in.)

7.1

Protein Family

Kinases & Inhibitors

Protein Subtype

Cytoplasmic Tyrosine Kinases

Qty/CS

1250 / Cs

1260 / Cs

2500 / Cs

10000 / Cs

Qty/Pk

125 / Pk

126 / Pk

500 / Pk

1000 / Pk

InChIKey

NTBLZMAMTZXLBP-UHFFFAOYSA-M

Product Cubic inch

401

Product Length (in.)

9.1

Product Size

100mg

Quantity

0.2mL (Total: 8-µmol)

100U (2U/-µL)

1mL

200 rxns

4 x 1mL (4 x 100 -µmol of 100mM solution)

100 x 50µL rxns

1000 Pack

1mL (Total: 40-µmol)

100 rxn

100 Units

1000

1kU

4 x 0.25mL (4 x 25 -µmol of 100mM solution)

50 reactions

500U (2U/µL)

1250 x 50++L rxns

200 x 50µL rxns

500U

Quantity

100 x 20Î¼L rxns

100U

125

2,500U

200µL

200U

250U

25U

5 x 1,200U

5 x 1mL

800μL (800 reactions)

250 x 50Î¼L rxns

1000U

125U

300µL

300µL (300 reactions)

3kg

400µL (400 reactions)

500mg

800µL

100µL

100mg

2,000U

20 g

5 x 1mL (Total: 200Âµmol)

50µL

Purityor QualityGrade

Molecular Biology Grade

ReactionFormat

Separate Components

SuperMix or Master Mix

Standalone

Recommended Storage

Keep cold

Store at -20°C

Pub Chem CID

74629

Research Category

Signal Transduction, Immunology

Other Research

Item Class Description

Reagents

IUPAC Name

2-acetylsulfanylethyl(trimethyl)azanium;iodide

Radioactive

ReactionSpeed

Standard

Fast

SensitivetoHeatInactivation

Yes

Sensitivity

Hygroscopic; Light sensitive

Shipping Conditions

Room Temperature

Shipping Weight(lbs)

Size

1,000µl

10,000u

100 units

1000 U

2,500 units

20 reactions

200µmol

25µmol

250 preps

25ml

500 units

500u

1,000 preps

10,000 units

20µg

40 reactions

40µmol each

5 mL

5,000u

9 × 2µg

1,000 reactions

100 reactions

100u

1mL

200 reactions

200 U

20ml

40µmol

50 Preps

5ml

10µmol each

12.5ml

2,500u

25µmol each

500 reactions

None

Size

1.5mL

50 mL

200µL

Sample Weight

10 ng of genomic DNA; or non-purified cell lysates

Select Product

0.2 ml hinged tube with cap, 96 Reactions

Multiwell Plate, 5 x 96 Reactions

SMILES

CC(=O)SCC[N+](C)(C)C.[I-]

Ship Length (in.)

Ship Width (in.)

Material

multiple

Polypropylene Co-Polymer (PPC)

Source

Crotalus adamanteus venom

Clostridium histolyticum

Tritirachium album

Bovine Liver

Max Gradient C

Max Temperature C

100

Species

Human

Storage Requirements

-20°C, Protect from moisture

4°C

<0°C

-20°C

Source

Egg White

Storage Temperature

+2 -> +25 C

Freezer: - 20 C

Room Temperature

Style

0.2 mL 96-Well PCR Plate

0.2 mL Thin-Wall

SufficientFor

20 reactions

100 reactions

STERILITY

Non-sterile

RTECS Number

FZ9865000

Sample Type

DNA, dsDNA

RNA

Shipping Condition

Approved for shipment on Wet or Dry Ice

Dry Ice

ShippingCondition

Dry Ice

Ships by Itself

YES

Min Gradient C

One Unit Contains

960 preps/kit

Optimal Reaction Temperature

37° C

Optimal ReactionTemperature

42° C

Type IISRe

TypeIISRe

Yes

Unit of Measure

Case

UNSPSC

41105506

Synonym

EC 2.7.1.40

s-acetylthiocholine iodide

Primer

Random Primers

Product Gross weight

14.5000g

Product Name

PCR Master Mix (2X)

Tests Per Kit

Product Weight (lbs)

Tube Color

Blue

Amber

Green

Natural

Red

Yellow

TargetGene

lymphocyte-specific protein tyrosine kinase

Type

Thin Wall PCR Tube

UoM Description

kit

Validated Application

Kinase Assay

Units per Case/Pack/Each

48 tip parks and 50 piercing tools

12 tubes

UoM

KIT

Volume Metric

0.2 mL

Warranty

2 years

Width in

27.5629

Reaction Format

SuperMix or Master Mix

Reaction Speed

Fast

Reaction Time

1.5-2 h

Refrigerated/Frozen

Frozen

Reverse Transcriptase

RevertAid

Shelf Life

120 Days

Shelf Life (months)

Sterile

Storage Conditions

-15 to -30Â°C

-20C

Ambient temperature in pouch with desiccant

Store the kit at ambient temperature. Once opened, completely reseal the pouch containing the Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended. Store recons

System Compatibility

LanthaScreen® Antibody, LanthaScreen® Substrate, Z'-LYTE™, Omnia® Bulk Substrate

Tag Position

C-Terminal

TargetEntry

LCK

Temperature Accuracy

50°C: ±0.25°C

TSCA

Yes

Tube Material

Polypropylene Co-Polymer (PPC)

UN Number

UN2811

UNSPSC Code

41105506

Width cm

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