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Volume

200µL

Accession Number

NP_005347

Auto Restart On Power Failure

Yes

Block Uniformity

Block at 55°C: ±0.3°C

Bottom Style

Conical

Brand

Life Sciences

Twist

Axygen®

Binding Capacity

12 µg Based on specified 15 µL aliquot of supplied magnetic bead suspension = 1.5 mg beads @ 100 mg/mL

CAS_NO

146986-50-7

856925-71-8

2304-81-6

39450-01-6

9003-98-9

50-07-7

9001-45-0

163795-75-3

9001-12-1

71519-72-7

9001-15-4

91079-46-8

Block Description

Four sets of 96 x 0.2 mL tubes or four 96-well plates

Cap Material

Polypropylene

Capacity

0.2 mL

Chemical Substance ID

329773119

329751672

329798458

Certificate of Analysis

Yes

Color

Colorless

Clear

White

Brown

Concentration

50 X

1 million copies/microliter

10 X

>=20?/mg

10 mM

>160U/mg

100 mM

Content AndStorage

• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
• 10 mM dNTP Mix

Store at -5 to -30°C.

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
Both Phusion HF and GC Buffers provide 1.5 mM MgCl2 in the final

Contains:
• 4 x 1.25 mL DreamTaq Green PCR Master Mix (2X), which includes DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
• 4 x 1.25 mL Nuclease-free water

Store at -20-°C.

Contains:
• 1 mL Phusion Blood Direct PCR Master Mix (2X)
• 40 µL Universal Control Primer Mix
• 0.5 mL 50 mM EDTA
• 1.5 mL 50 mM MgCl2
• 0.5 mL DMSO
• 1.25 mL Water, nuclease-free
• 50 µg O'GeneRul

Contains:
• 4 x 1.25 mL DreamTaq Hot Start Green PCR Master Mix (2X)
• 4 x 1.25 mL Nuclease-free water

Store at -5 to -30°C.

Includes:

• 2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration)
• Water, nuclease-free
• DMSO

Store at -20°C.

Contains:
• 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 2 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (sufficient f

Includes:
• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

S

Contains:
• 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 µL reactions.

G?° 4 x 1.25 mL 2X Phire Hot Start II PCR Master Mix
G?° 1 x 0.5 mL DMSO
G?° 4 x 1.25 mL Water, nuclease free

Store at -20°C.

The RevertAid RT Kit contains:
• RevertAid Reverse Transcriptase
• RiboLock RNase

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 ++M Forward GAPDH Primer, 10 ++M Reverse GAPDH

CompatibleBuffer

10x Buffer G

10x Buffer Tango

10x FastDigest Buffer/FastDigest Green Buffer

Unique Buffer (10x Buffer AarI)

Unique Buffer (10x Buffer SacI)

Concentration

Color of florescence

Orange

Far-Red

Country of Origin

Content And Storage

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl2 solution

Both Phusion Green HF and GC Buffers provide 1.5

Contains:
• 10 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 2 x 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 10 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (suffic

Depth Cm

Depth In

Enzyme

AdeI

Eco147I

Eco81I

PagI

Psp1406I

Xba I

Xho I

Eco31I

EcoR I

Kpn2I

Bst1107I

MunI

AarI

BcuI (SpeI)

BglI

Dpn I

AatII

PacI

BpiI

Hind III

MssI

BshTI

Bgl II

Esp3I (BsmBI)

Mph1103I

Pst I

Sca I

Kpn I

KspAI

LguI (SapI)

Nhe I

Not I

Pfl23II

SacI

MreI (Sse232I)

Nde I

XmaJI

BpiI (BbsI)

Pvu I

Pvu II

Fidelityvs Taq

1 X

Components

20µg Mass spec grade lyophilized Trypsin/Lys-C Mix, 500µL Resuspension Buffer

For Use With Application

Used to breaks phosphodiester bonds, catalyze the hydrolysis of various Nucleotide polyphosphates

Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases

For the routine subcloning of PCR products

In-solution Protein digestion, in-solution digestion of proteolytically resistant Proteins

PCR product analysis

For PCR application

For tissue dissociation combined with other Enzymes such as Elastase, Trypsin/Papain

Used to lyse E. coli for preparation of plasmid DNA from transformed cell cultures

Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)

Fluorescence Wavelength Max_17

570 nm [m]

670 nm [m]

Format

Lyophilized Powder

0.2mL Tube with hinged cap

Solid

Format

Crystalline Powder

Liquid

Formula

C₃₆H₇₁NO₃

C₁₅H₁₈N₄O₅

Na₂O₃PNHC(=NH)N(CH₃)CH₂COOH · 4H₂O

C14H21N3O · 2HCl · H2O

Formula Weight

975.15 Daltons

949.11 Daltons

Expression System

Baculovirus

GCRichPCRPerformance

Low

High

Medium

DetectionMethod

SYBR

For Use With Equipment

7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus System, StepOne System

EC Number

231-791-2

232-606-8

232-667-0

232-582-9

254-457-8

293-434-7

232-585-5

FidelityvsTaq

2 X

25 X

52X

52 X

Form

liquid

Export control classification number

EAR99

Extinction Coefficient in Methanol Max

150000 M-1cm-1

250000 M-1cm-1

GCRich PCRPerformance

High

Medium

Functional Testing

Each batch of PuReTaq Ready-To-Go PCR Beads is tested in a real-time PCR assay to ensure its ability to generate a specific PCR product with Î» DNA; and with negative control samples (untemplated reactions) to ensure the lowest possible levels of either e

Gel Compatibility

Acrylamide Gels, Agarose Gels

Gene Alias

LCK

Hot Start

Built-In Hot Start

Hazardous

Yes

GeneID Entrez

3932

Height Cm

53.5

Height In

21.0625

Heated Lid Temperature

35 to 115°C, or off

Harmonized Code US

3822005090

Heated Lid Pressure

Adjustable

Includes

1.2µg pGEM-T easy vector (50ng/µL), 12µL control insert DNA (4ng/µL), 200µL rapid ligation Buffer (2X), 100U T4 DNA Ligase

Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25µL final volume, the concentration of each dNTP

Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL); 5X Phusion Green HF buffer (provides 1.5 mM MgCl2 in the final 1X concentration); 5X Phusion Green GC buffer (provides 1.5 mM MgCl2 in the final 1X concentration); DMSO; 50mM MgCl2 solution

2 x 1250µL GoTaq Green MasterMix, 2X, 2 x 1250µL Nuclease free Water, blue dye and a yellow dye, GoTaq DNA Polymerase, dNTPs, MgCl2 and reaction Buffers in a single tube

2X Phire Hot Start II PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease free; DMSO

2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease-free; DMSO

Includes Labelor Dye

Yes

Kinase Group

Tyrosine Kinases

Max Heating Rate

3.4°C per second

MDL Number

MFCD00132129

MFCD02259139

MFCD00130830

MFCD00130918

MFCD00284715

MFCD00130876

MFCD08460907

MFCD00150192

MFCD00130629

Methylation Sensitivity

Not CpG methylation-sensitive, dcm methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive

Min Temperature C

Molecular Weight

334.33

0.0

292.33

327.14

565.95

115000

60.6 kDa

No of Reactions

Polymerase

Phusion Hot Start II DNA Polymerase

Phusion Hot Start II High-Fidelity DNA Polymerase

Phire Hot Start II DNA polymerase

Phusion U Hot Start DNA Polymerase

DreamTaq DNA Polymerase

Taq DNA Polymerase

Phusion Blood II DNA Polymerase

Protein Tag

His Tag

Quantity

100 x 20Î¼L rxns

200U

5 x 1,200U

5 x 1mL

125U

300µL

800µL

200µL

400µL (400 reactions)

100mg

2,000U

50µL

100µL

250 x 50Î¼L rxns

1 Kit

300µL (300 reactions)

3kg

500mg

25U

800μL (800 reactions)

Quantity

100U

4 x 1mL (4 x 100 -µmol of 100mM solution)

100 rxn

1000U

100 x 50µL rxns

100U (2U/-µL)

100 Units

4 x 0.25mL (4 x 25 -µmol of 100mM solution)

500U (2U/µL)

200 x 50µL rxns

500U

1250 x 50++L rxns

20µg

100 Reactions

1mL (Total: 40-µmol)

20 reactions

50 Reactions

200 rxns

Radioactive

FALSE

SensitivetoHeatInactivation

Yes

Size

20 reactions

1,000 reactions

100u

20ml

1 system

10,000u

1,000 preps

20microg

250 preps

40micromol

25micromol

1,000microl

200micromol each

25micromol each

5,000u

2,500u

250 preps/100 extractors

10µmol each

12.5ml

50 preps/25 extractors

500 reactions

100 reactions

200 reactions

50 preps

5ml

9 x 2microg

40 reactions

40µmol each

200microl

25ml

500u

Storage Requirements

-30 to -10°C

−30 to −10°C

<0°C

Ambient temperature in pouch with desiccant

-20°C

4°C

Type IISRe

Inhibitors

Gluctathione, Cysteine and Ascorbic Acid

Cysteine, EDTA or o-phenanthroline, alpha2-macroglobulin - a large plasma glycoprotein, alpha2-macroglobulin, alpha1-antitrypsin

Item Class Description

Reagents

Number of Tests

55 reactions

OptimalReactionTemperature

37° C

Pack size

100 nmol

25 nmol

55 reactions

96 reactions

Packaging

Poly Bottle

Product Line

DreamTaq

Phire

Phusion

RevertAid

FastDigest

Maxima

Product Type

Proteinase K

Phosphodiesterase I

Trypsin/Lys-C Mix

Collagenase Type 4

Lysozyme

Memory

1000 programs max

Labelor Dye

Unconjugated

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

ReactionFormat

Standalone

Separate Components

SuperMix or Master Mix

ReactionSpeed

Fast

Standard

Sample Type

DNA, dsDNA

RNA

Kit Contents

Ready-To-Go GenomiPhi V3 cakes, 1 Ã— 96-well plate; 2x Denaturation buffer, 1 Ã— 1.2 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps

Bulk first-strand cDNA reaction mixes (5)DTT solutionpd(N)₆ primerNot I-d(T)18 bifunctional primermRNA standardRNase-free water

Room-temperature stable beads containing ~ 2.5 units of recombinant PuReTaq DNA polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction buffer. When a bead is reconstituted to a 25 Î¼L final volume, the concentration of each dNTP is 200 Î¼M in10

Label Type

Unlabeled

Shipping Temperature

-80C

ShippingCondition

Dry Ice

MethylationSensitivity

Not dam methylation-sensitive, dcm methylation-sensitive, CpG methylation-sensitive

Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not CpG methylation-sensitive, dcm methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive

Number Of Blocks

PAC Code Description

Chemagic Kits MSM I

No ofReactions

200 reactions

100

Product Gross weight

14.5

Order Information

Custom options of the product, including larger pack sizes, are available. Please contact your local sales representative or Technical Support.

Additional pack size available on request, please inquire.

Includes LabelorDye

Yes

Power Hz

50/60

Power Vac

100 to 240

Promoter

Sp6 and T7

Material

Synthetic RNA

Kinase Family

SRC Kinase Family

Overhang

3'-A

Blunt

PAC code

6814

Qty/CS

1250 / Cs

10000 / Cs

Research Category

Signal Transduction, Immunology

Other Research

Sample Weight

10 ng of genomic DNA; or non-purified cell lysates

Size

1.5mL

Mass

10 µg

Product Family

Axygen® PCR Tubes and Caps

Axygen® PCR Strip Tubes

Select Product

Multiwell Plate, 5 x 96 Reactions

0.2 ml hinged tube with cap, 96 Reactions

LabelorDye

Unconjugated

Shipping Condition

Approved for shipment on Wet or Dry Ice

Dry ice

Species

Human

Protein Subtype

Cytoplasmic Tyrosine Kinases

SufficientFor

20 reactions

100 reactions

Storage Temperature

Room Temperature

-80C

Max Gradient C

Max Temperature C

100

Purityor QualityGrade

Molecular Biology Grade

Optimal ReactionTemperature

42° C

p H

9.8 to 10.4.5

Primer

Random Primers

Source

Clostridium histolyticum

Tritirachium album

Crotalus adamanteus venom

Reaction Speed

Fast

TargetEntry

LCK

Temperature Accuracy

50°C: ±0.25°C

Min Gradient C

TypeIISRe

Yes

Width in

27.5629

Source

Egg White

One Unit Contains

1 Each

OptimalReaction Temperature

37° C

PCRMethod

qPCR

Optimal Reaction Temperature

37° C

TargetGene

lymphocyte-specific protein tyrosine kinase

Product Gross Weight Desc

Validated Application

Kinase Assay

Qty/Pk

1000 / Pk

125 / Pk

Quantum Yield in Methanol

> 0.28

> 0.15

Reaction Time

1.5-2 h

Reagent type

Fluorescent Labeling Reagents

Reverse Transcriptase

RevertAid

Protein Family

Kinases & Inhibitors

Output

USB port

Storage Buffer

50mM Acetic Acid (pH <3) Resuspension Buffer

Storage Conditions

-15 to -30Â°C

Ambient temperature in pouch with desiccant

Store the kit at ambient temperature. Once opened, completely reseal the pouch containing the Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended. Store recons

Physical State

solid

Reaction Format

SuperMix or Master Mix

Refrigerated/Frozen

Frozen

Product Size Avg

> 10 kb

Volume

100 uL

Product Type Specs

KOD Hot Start Master Mix

Protein Form

Full Length, Recombinant, Cytoplasmic

Restriction Site

BstZI, NotI and EcoRI

UoM Description

kit

Vector

pGEM-T easy vector I

Sterile

Tag Position

C-Terminal

System Compatibility

LanthaScreen® Antibody, LanthaScreen® Substrate, Z'-LYTE™, Omnia® Bulk Substrate

Width cm

UoM

KIT

Tests Per Kit

Units Each

1 unit

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