Over the past few years, SNAP-Tag technology combined with TR-FRET has paved the road to the development of many non-radioactive, no-wash binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag and subsequently labeling them with Terbium. PT8HALONEO is a backbone containing the gene for the HALO tag, a promoter a neomycin resistance gene and MCS (Multiple Cloning Site).
Tag-lite assays offer many features and applications over traditional receptor/ligand binding assays:
- Non-radioactive
- Homogeneous and filtration-free
- Ready-to-use kits and reagents for binding assays
- Does not alter the receptor pharmacology
- Peer-reviewed, validated technology
- Thorough kinetic studies with true Kd and Ki values, association (Kon) and dissociation (Koff) rate constants
- Screening and profiling of biologics and large molecules