PhenoVue Multi-organelle Staining Kit includes five PhenoVue probes to visualize nuclei, mitochondria, lysosomes, actin, and lipid droplets. These organelles play crucial roles in various cellular processes and interact with each other in intricate ways to maintain cell homeostasis and carry out specific cellular processes.
The combination of specific, bright and photostable PhenoVue dyes enables the ability to perform a 5-plex experiment with maximum spectral separation and a significantly reduced spectral overlap.
Each PhenoVue probe has been extensively validated and carefully optimized for excellent batch-to-batch reproducibility.
The PhenoVue Multi-organelle Staining Kit offers a straightforward and user-friendly protocol to facilitate the experimental procedure. Avoiding the use of living cells, it provides a convenient alternative method for phenotypic screening approaches such as the well-established cell painting protocol.
Kit Components / Quantity:
- PhenoVue Hoechst 33342 Nuclear Stain / 1 vial of 70 µL (50000x)
- PhenoVue 493 Lipid Stain / 1 vial of 30 µL (4000x)
- PhenoVue Fluor 400LS - Phalloidin / 1 vial of 30 µL (400X)
- PhenoVue anti-HSP60 antibody 100X / 1 vial of 100 µL (100x)
- PhenoVue anti-LAMP1 antibody 100X / 1 vial of 100 µL (100x)
- PhenoVue Fluor 555 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed / 1 vial of 100 µL (100x)
- PhenoVue Fluor 647 - Goat Anti-Rat Antibody Highly Cross-Adsorbed / 1 vial of 50 µL (200x)
- PhenoVue Dye Diluent A (5X) / 1 vial - 8 mL (5X)