NEXTFLEX Cas9-gRNA Mito Depletion Enzyme (Human) - 50 units

The NEXTFLEX™ Cas9-gRNA Mito Depletion Enzyme (Human) is a standalone, flexible CRISPR-Cas9 module that removes mitochondrial derived sequences from human NGS libraries. It can be added at the double-stranded DNA stage of existing ATAC-seq, DNA-seq, bulk RNA-seq, or single-cell workflows, reducing mitochondrial background so more reads are directed on nuclear genes, regulatory elements, and other loci of interest. Supplied as a pre-complexed Cas9–guide RNA mixture, the NEXTFLEX Cas9-gRNA Mito Depletion Enzyme (Human) contains 657 guide RNAs targeting 17 mtDNA-encoded genes and selected nuclear mitochondrial pseudogenes. One unit is defined as the amount required to deplete 1–10 ng of target double-stranded DNA library of at least 400 bp. Designed for broad compatibility, it delivers efficient mitochondrial depletion across diverse human library types without locking you into a particular library preparation kit or sequencing platform