NEXTFLEX Cas9-gRNA Globin Depletion Enzyme (Human) - 250 units

The NEXTFLEX™ Cas9-gRNA Globin Depletion Enzyme (Human) is a standalone, flexible CRISPR-Cas9 module designed to remove globin-derived sequences from human blood RNA-seq libraries. It can be dropped into existing bulk or single-cell RNA-seq workflows at the double-stranded DNA stage, reducing hemoglobin background, so a greater share of reads is directed toward biologically informative transcripts and improved transcriptome coverage. Supplied as a pre-complexed Cas9-guide RNA mixture, the NEXTFLEX Cas9-gRNA Globin Depletion Enzyme (Human) contains 30 guide RNAs targeting the HBA1, HBA2, HBB, and HBD loci. A unit is defined as the amount required to deplete 1 - 10 ng of target dsDNA. Developed to be highly cost-competitive and widely compatible, it delivers efficient globin depletion without locking you into a particular library prep kit.