The NEXTFLEX small RNA sequencing kit v4 uses patent-pending technology to provide a completely gel-free small RNA library preparation solution for Illumina and Element sequencing platforms.
- Completely gel-free protocol from purified miRNA or as little as 1 ng total RNA, no PAGE cleanup required
- Automation-ready on Sciclone™ G3 NGSx, Sciclone G3 NGSx iQ™, and Zephyr™ G3 NGS workstations for walk-away scalability
- Low-bias ligation plus dimer reduction chemistry improve data quality from challenging biofluids (plasma, serum, urine, CSF) and extracellular vesicles.
- Internal benchmarking shows up to a 45% increase in unique miRNA species detected from as little as 1 ng of total RNA, supporting robust differential-expression analysis and novel small RNA discovery
- Libraries ready in ~ 6 hours, supporting rapid biomarker and functional genomics studies
- Includes 384 Unique Dual Index (UDI) barcodes for high-throughput multiplexing and minimal index hopping on Illumina®/Element® platforms
- Quality assured through rigorous testing of every lot
Explore our Dharmacon™ miRNA modulation reagents to enhance or inhibit miRNA levels as part of your functional studies.