- 10 ng – 5 µg total starting RNA
- Magnetic bead-based protocol
- No organic solvents or precipitation step required
- Optimized for use with 10 ng – 5 µg of total RNA as starting material
- Versions are available for automated and manual isolation of the mRNA transcriptome
- Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes, thereby eliminating the need for precipitation. These beads have been tested with the?NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.