Equipment and Instruments > Genomics Equipment > DNA Sequencing Devices

NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0

Supplied by REVVITY HEALTH SCIENCES INC

  • NOVA-4203-03 | The NEXTFLEX™ 16S V4 Amplicon-Seq Kit 2.0 prepares sequencing libraries that amplify the V4 hypervariable region of the 16S rRNA gene for microbiome profiling. The two-step PCR workflow with minimal cleanups maximizes recovery and produces multiplexed amplicon libraries that do not require custom sequencing primers. Libraries are compatible with paired-end 2×300 sequencing on Illumina® MiSeq® and Element® Aviti® sequencers, using overlapping reads across V4 for accurate taxonomic assignment. Typical input is 1–50 ng of high-quality genomic DNA, enabling a fast, reliable amplicon sequencing workflow for bacterial community profiling. Choosing the V4 region provides broad, well-characterized bacterial coverage with the widely used 515F/806R primers. The resulting overlapping 2×300 reads support accurate amplicon sequence variant (ASV) calling and are highly compatible with established reference databases and analysis pipelines. The kits include up to 384 barcoded PCR II primers to improve the efficiency of your workflow through high throughput indexing and pooling. In the product listing, the “S” variants include access to 16S analysis through Cosmos-Hub, which runs a DADA2-based 16S pipeline with SILVA taxonomy to generate quality-controlled ASV tables and interactive visualizations.
  • NOVA-4203-03 | The NEXTFLEX™ 16S V4 Amplicon-Seq Kit 2.0 prepares sequencing libraries that amplify the V4 hypervariable region of the 16S rRNA gene for microbiome profiling. The two-step PCR workflow with minimal cleanups maximizes recovery and produces multiplexed amplicon libraries that do not require custom sequencing primers. Libraries are compatible with paired-end 2×300 sequencing on Illumina® MiSeq® and Element® Aviti® sequencers, using overlapping reads across V4 for accurate taxonomic assignment. Typical input is 1–50 ng of high-quality genomic DNA, enabling a fast, reliable amplicon sequencing workflow for bacterial community profiling. Choosing the V4 region provides broad, well-characterized bacterial coverage with the widely used 515F/806R primers. The resulting overlapping 2×300 reads support accurate amplicon sequence variant (ASV) calling and are highly compatible with established reference databases and analysis pipelines. The kits include up to 384 barcoded PCR II primers to improve the efficiency of your workflow through high throughput indexing and pooling. In the product listing, the “S” variants include access to 16S analysis through Cosmos-Hub, which runs a DADA2-based 16S pipeline with SILVA taxonomy to generate quality-controlled ASV tables and interactive visualizations.
Average lead time: Call for Lead Time
ADD TO CART
Need Assistance?
Jump to Section
Product Description
Specifications
Additional Information
Media
Product Description

  • Straightforward workflow: two PCR steps with minimal cleanups; no custom sequencing primers required.
  • Cleanup beads included: NEXTFLEX NGS Cleanup Beads are supplied with the kit, allowing PCR cleanups without purchasing separate beads. This reduces consumables cost and simplifies purchasing.
  • Accurate taxonomic assignment: overlapping paired-end 2×300 reads across the V4 region on Illumina MiSeq and Element Aviti sequencers support robust error correction and ASV recovery.
  • Flexible Input: validated conditions for 1–50 ng genomic DNA help maintain consistency across samples.
  • Multiplexing Options: from 12 to 384 PCRII Barcodes are available to support projects of all sizes.
  • Analysis: product variants with “S” in their SKU include Cosmos-Hub 16S analysis (DADA2 with SILVA) to generate quality-controlled ASV tables and interactive reports.
  • Automation: protocols are available for Sciclone™ NGS, NGSx, and Sciclone G3 NGSx iQ™ Workstations to automate 16S sequencing.


Use this kit when you want a dependable 16S V4 library prep that fits standard 2×300 sequencing on Illumina MiSeq or Element Aviti sequencers and hands off cleanly to downstream taxonomic analysis, including the Cosmos-Hub.

Specifications
Inventory Number:
PERNOVA420303EA
Part Number:
NOVA-4203-03
Supplier:
REVVITY HEALTH SCIENCES INC
Supplier's Lead Time:
Call for Lead Time
Quantity:
96 per EA
Contract Availability:
Country of Origin:
United States
Business Unit:
DX
Disclaimer:
For research use only. Not for use in diagnostic procedures.
Export control classification number:
EAR99
Harmonized Code EU:
38220000
Harmonized Code US:
3822190080
Item Class Description:
Reagents
One Unit Contains:
96 rxns
PAC Code:
6074
Product Gross weight:
0.2000g
Product Gross Weight Desc:
g
Radioactive:
No
Shipping Condition:
Shipped in Dry Ice
Storage Temperature:
-30 -> - 16 C
UNSPSC:
41116006
UNSPSC Code:
41116006
Additional Information

Contact the GSS Team Today!
GSS-Sales@govsci.com | 800.248.8030 | www.govsci.com

Government Scientific Source

Government Scientific Source is a leading distributor of laboratory products and equipment dedicated to serving federal, state, and local governments.

ISO 9001:2015 Certified
Copyright 2026, Government Scientific Source. All Rights Reserved.