ATP [?-32P] is a type of ATP where the gamma phosphate group is tagged with a radioactive isotope of phosphorus, phosphorus-32 (32P).
Because the gamma phosphate is the one that's most often transferred during phosphorylation reactions, the radioactive tag allows scientists to track its movement. This makes ATP [?-32P] a valuable tool for:
- Kinase assays: Kinases are enzymes that add a phosphate group to a molecule. By using ATP [?-32P], researchers can measure the activity of these enzymes by seeing how much of the radioactive phosphate is transferred to a target molecule.
- Nucleic acid labeling: It is used to label the 5' end of DNA or RNA strands, which is helpful in various molecular biology techniques like hybridization assays and sequencing.
- Studying cellular signaling pathways: Since phosphorylation is a key mechanism in many cellular signaling pathways, using ATP [?-32P] allows for the precise study of these processes.
250 µCi quantities of ATP, [?-32P]- 3000Ci/mmol 10mCi/ml EasyTides™ are available for your research.
EasyTides products are provided in a proprietary buffer that contains a dye to aid in pipetting and can be stored at 4°C (avoiding freeze-thaw cycles). BLU products are packaged in a lead-free container ("pig").