Correct buffer conditions are essential for optimal cell lysis and extraction of proteins. The composition of the phospho / total protein lysis buffer 2 has been carefully optimized for the quantification of protein expression and phosphorylation. You may refer to the lysis buffer compatibility table to find out which assay works best with buffer 2.
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays