HTRF Phospho-ALK (Tyr1604) Detection Kit, 50,000 Assay Points

ALK (anaplastic lymphoma kinase) is a transmembrane tyrosine kinase belonging to the superfamily of insulin receptors. ALK regulates several pathways involved in cell survival, proliferation, and cell cycling, including the AKT/PI3K,MAPK/ERK, STAT3 and NFkB pathways.The ALK ligands, pleiotrophin (PTN) and midkine(MDK), induce ALK phosphorylation and activation on  multiple Tyrosine including Y1604. ALK deregulation is associated with  various cancers such as NSCLC, and neuroblastomas. Indeed, oncogenic ALK is driven by i) gene translocations and subsequent intracellular ALK fusion proteins (eg, EML4-ALK, NPM1-ALK, TPM3-ALK, VCL-ALK); ii) mutations (eg G263, R401, R551, P968 and E1242). Oncogenic ALK is constitutive active which results in uncontroled activation of downstream signaling pathways, leading to tumor cells survival and proliferation.