Acetyl-CoA carboxylase (ACC 1/2) is an important regulator of fatty acid metabolism. By catalyzing the carboxylation of acetyl-CoA to malonyl-CoA, ACC serves as a novel biomarker or drug target in diabetes and obesity research. Phosphorylation by AMPK at Ser-79 inhibits ACC enzymatic activity. This cell-based assay enables simple yet sensitive and efficient quantification of ACC 1/2 phosphorylation on Serine 79, and offers enhanced convenience over ELISA or WB assays.
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays