steadylite plus™ is a homogeneous, high sensitivity luciferase reporter gene assay system quantitation of firefly luciferase activity in mammalian cells. steadylite plus was designed to provide an extended signal half-life of more than 4 hours, making it detection system of choice batch processing systems where a large number of processed plates can wait in stacks before measurement. steadylite plus is suitable 96-, 384-, or 1536-well microplate formats, and is ideal for:
- High throughput assays employing a batch processing methodology
- Any experimental design where detection is delayed, requiring a strong, steady, and long-lived luminescent signal
As with all members of Revvity's Lites suite of luciferase detection reagents, steadylite plus offers superior performance in an easy-to-use format, providing following benefits:
- Convenient One-Step Processing: No washes required - simply mix, incubate and read
- Enhanced Stability: All lites reagents are designed extended storage at 2-8°C
- No DTT: Unlike most luciferase detection reagents, lites products do not contain DTT, eliminating toxic hazards and need hood work
- Excellent Z' Values: All lites products offer robust, sensitive performance, with high signal-to-background ratios and excellent Z' values