ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so monitoring ATP is a good indicator of cytotoxic, cytostatic and proliferation effects.
Our ATPlite 1step ATP luminescence assays use patented technologies to measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.
Features and benefits:
- Simple and reproducible: only one reagent addition step, no separation steps
- Suitable for both 96- and 384-well microplates
- Linear correlation between cell number and luminescent signal: up to 50,000 cells per well for 96-well microplates and 12,500 cells per well for 384-well microplates
- Designed for continuous process systems: luminescence should be measured between 0 and 30 minutes after reagent addition and plate shaking
- Homogeneous: no cell harvesting or centrifugation required
- High sensitivity: able to detect down on one cell per well
- Reduced Phenol Red dependency
- High light-output: assay can be used with less sensitive luminescence readers such as multi-label readers
- Fast: no luminescence signal stabilization time required
An alternative format of this assay is also available. The ATPlite assay has separate cell lysis and luminescent signal generation steps. This allows for a more stable signal, with half-life of at least 4 hours.