The PG-Seq™ Rapid kit v2 has been developed to analyze picogram quantities of DNA (single/multi-cells or low template DNA) from an embryo biopsy for preimplantation genetic testing.
Updated, streamlined, simple PGT protocol
From DNA to data, the PG-Seq™ Rapid kit v2 includes all reagents required for cell lysis, whole genome amplification, indexing along with the PG-Find™ analysis software for automatic calling of aneuploidy and copy number variants.
Accurate Copy Number Detection
Extensively tested using over 100 cell lines and genomic DNA samples with known ploidy. Tested from whole chromosome aneuploidies down to segmental aberrations 7Mb in size with 30pg of genomic DNA or 5 cell fibroblast samples, representative of a trophectoderm biopsy. See PG-Seq™ Rapid kit v2 app note for more information.
Flexible Kit Format
The PG-Seq™ Rapid kit v2 has 48 rxns Two sets of 48 unique indexes are available, allowing up to 96 sample multiplexing (or up to 384 sample multiplexing available by custom request). Larger 40 µL WGA PCR 1 reaction volume enable options for alternative downstream processing. High WGA PCR 1 yield of 2-4 µg total DNA with only 23 cycles of PCR.