The DELFIA® Enhancement Solution is an acidic chelating detergent solution intended for use in the quantitative determination of Eu3+/Sm3+ when using a time-resolved fluoroimmunoassay. The solution dissociates Eu3+/Sm3+ from solid phase bound Eu-labeled antibodies or proteins during a time period of a few minutes to form a homogeneous and highly fluorescent micellar chelate solution. The solution allows highly sensitive Eu3+/Sm3+ measurements to be made when using the time-resolved fluorometer. The DELFIA® Enhancement Solution is also used as part of Tb3+/Dy3+ measurement together with DELFIA® Enhancer.
Because DELFIA TRF chelates are not intrinsically fluorescent, this solution (an acidic chelating detergent solution) is added to the microplate wells after a wash step to develop the fluorescence signal. This provides highly sensitive Eu and/or Sm measurements. Suitable for use in all multianalyte assays.