The XFM-2X Phantom Mouse calibration tool features an outer material engineered to replicate the optical properties of biological tissue. This design ensures that users experience light scattering effects that closely resemble those observed in live subjects during imaging procedures. Additionally, the phantom’s surface coloration is designed to emulate tissue autofluorescence, providing a realistic background signal. Included with the XFM-2 are multiple plastic rods infused with distinct fluorescent dyes, allowing users to practice and refine fluorescence imaging techniques across a range of spectral profiles.