GDP-Glo(TM) Glycosyltransferase Assay

The GDP-Glo™ Glycosyltransferase Assay is a homogeneous, single-reagent-addition method to rapidly detect GDP formation in glycosyltransferase reactions. After the reaction, an equal volume of GDP Detection Reagent is added to simultaneously convert the GDP product to ATP and generate light in a luciferase reaction. The light generated is detected using a luminometer, and the light output is proportional to the concentration of GDP from low nM to 25microM. Luminescence can be correlated to GDP concentration by using a GDP standard curve. The assay is easy to use and highly sensitive, two features that are desirable and essential for measuring the activity of different GDP-sugar-utilizing glycosyltransferases such as fucosyltransferases. Therefore, the GDP detection assay uses less enzyme in glycosyltransferase reactions. The GDP-Glo Assay is performed in a single well of a multiwell plate and can be used to detect glycosyltransferase activity in as little as a 5microl reaction.