GSK3 Beta Kinase Enzyme System

Full-length rhGSK3 beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3 beta is a serine-threonine protein kinase that was originally identified as the kinase that phosphorylates and inhibits glycogen synthase. GSK3 beta is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin, modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK3 beta into CHO cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau at all sites investigated with phosphorylation-dependent anti-tau antibodies. Kinase: GSK3 beta, 10ug (Human, recombinant full-length). MW: ~73kDa. Substrate: GSK3 Substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE); derived from human muscle glycogen synthase 1 (amino acid 636-661). Reaction Buffer, DTT. GSK3 beta NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/2932/.