RiboMAX Large Scale RNA Production System-SP6, 1 system

The RiboMAX Large Scale RNA Production Systems consistently produce 2-5mg/ml of RNA in a 1ml reaction, about 10- to 20-fold more RNA than is produced with the standard Riboprobe System transcription reaction. The RiboMAX System reactions differ from those of the Riboprobe Systems in three primary ways: a HEPES (pH 7.5) buffer is used rather than a Tris-HCl (pH 7.9) buffer; rNTP and magnesium concentrations are elevated at levels appropriate for either SP6 or T7 RNA polymerase; and inorganic pyrophosphatase is included in the reaction. RNAs synthesized with the RiboMAX System perform better for in vitro translation in rabbit reticulocyte translation systems than RNA synthesized by standard methods. The reduction of components inhibitory to translation may be advantageous for other applications requiring biologically active RNA. Because the RiboMAX Systems produce large quantities of RNA, these systems are not recommended for the generation of high-specific-activity RNA probes. Note: Use of the RiboMAX System for production of capped transcripts requires separate purchase of the Ribo m7G Cap Analog (Cat. P1711).