NanoBRET(TM) BRD4/Histone H3.3 Interaction Assay

Bromodomain (BRD)-containing proteins are critical components of nuclear protein complexes involved in recruitment of chromatin-modifying enzymes and transcriptional regulation of acetylated chromatin. The protein:protein interaction of BRD-containing proteins with acetylated histones is an important method of epigenetic regulation critical for cell health and development and of great interest for drug targeting because dysfunction in BRD modulation was implicated as a critical event in disease formation. The NanoBRET Bromodomain BRD4/Histone Interaction Assays enable interaction studies of BRD-containing proteins with full-length histones in the context of natural chromatin. In addition to the full-length BRD protein, the BRD fragment alone is included for users that may want to understand the interaction of this isolated domain. NanoBRET technology is dependent upon energy transfer from a luminescent donor (NanoLuc luciferase) to a fluorescent acceptor (HaloTag NanoBRET 618 Ligand).