Nano-Glo(R) Dual-Luciferase(R) Reporter Assay

The Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System is a homogeneous reagent system to sequentially detect the activities of firefly (Photinus pyralis) and NanoLuc luciferase (Nluc) from a single sample. The firefly luciferase (Fluc) activity is measured first using ONE-Glo EX Luciferase Assay Reagent. NanoDLR Stop & Glo Reagent is added to quench the firefly signal and provide the furimazine substrate needed to measure Nluc activity. This convenient format generates stable glow-type luminescent signals for both reporters directly from cells with no lysis or cell preconditioning steps required. Potent Fluc inhibition coupled with the high-intensity Nluc luminescence create a dual assay in which both reporters have maximum sensitivity in an assay format that is both easy-to-use and flexible. The NanoDLR workflow is compatible with assays or screens in any plate size, supports batch processing, and is ideal for any luminometer with no specific filter or injector requirements.