pNLF1-secN [CMV/Hygro] Vector

The NanoLuc protein fusion vectors enable simple generation of N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. The pNLF Vector series uses using traditional cloning with a multiple cloning site (MCS) to generate N- or C-terminal fusions to the full-length Nluc protein with the pNLF1-N or pNLF1-C [CMV/Hygro] Vectors or attach secreted Nluc to the N-terminus of the protein of interest with pNLF1-secN [CMV/Hygro] Vector. Also make NanoLuc protein fusions with the pF Vector series: Generate N- or C-terminal Nluc fusion proteins using the Flexi Vector Cloning System—a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, that provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence.