GoTaq(R) Hot Start Green Master Mix

GoTaq Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq Hot Start Polymerase, magnesium, dNTPs and buffer. Set up reactions in less than a minute at room temperature; just add your template, water and primers. GoTaq Hot Start Polymerase contains the high-performance GoTaq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94-95 degrees C for two minutes, enabling hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70 degrees C. GoTaq Hot Start Polymerase exhibits 5' --> 3' exonuclease activity. The master mixes are available in green or colorless formats. The green master mix contains a compound that increases sample density, so samples sink easily into wells of an agarose gel, and two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis.