pF3K WG (BYDV) Flexi(R) Vector 20ug

Flexi Vector System is a simple, powerful, directional cloning method for protein-coding sequences. Based on 2 rare-cutting restriction enzymes, SgfI & PmeI, it provides rapid, efficient and high-fidelity transfer of protein-coding regions between Flexi Vectors without resequencing. pF3A/K WG Vectors are designed with special sequences for maximal cell-free protein expression in a specific system. They contain sequences from barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest. The BYDV elements interact with each other, form a closed loop and act synergistically to stimulate translation in wheat germ extract, bypassing mRNA cap and polyadenylation dependencies. Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during subcloning. The Flexi System replaces the barnase gene with your insert. These vectors cannot be cultured in normal laboratory strains of E. coli without an insert.