CytoTox-Glo(TM) Cytotoxicity Assay

CytoTox-Glo Assay is a luminescent cytotoxicity assay to measure the relative number of dead cells in cell populations. Using a luminogenic cell-impermeant peptide substrate, the assay measures the extracellular activity of a distinct intracellular protease activity (dead-cell protease) when the protease is released from membrane-compromised cells. The liberated product is measured as glow-type luminescence generated by Ultra-Glo Recombinant Luciferase provided in the assay reagent. The amount of luminescence directly correlates with the percentage of cells undergoing cytotoxic stress. With the addition of a lysis reagent (provided), the CytoTox-Glo Assay also can deliver the luminescent signal associated with the total number of cells in each assay well. Calculate viability by subtracting the luminescent dead-cell signal from the total luminescent value, thus normalizing assay data to cell number and mitigating assay interferences that may lead to erroneous conclusions.