ONE-Glo EX Luciferase Assay System, 10 x 100ml

High- or ultrahigh-throughput quantitation of firefly luciferase expression in mammalian cells is commonly performed by measuring luminescence from 96-, 384- or 1,536-well plates. The ONE-Glo EX Luciferase Assay System provides both the high sensitivity and long-lived luminescence required to batch-process multiple plates in these assay formats. The ONE-Glo EX Assay retains many of the beneficial aspects of the ONE-Glo Assay, using 5´-fluoroluciferin substrate with an add-mix-read, or homogeneous, protocol. Extending the properties of ONE-Glo Reagent, ONE-Glo EX Reagent employs a new assay chemistry to increase the stability of both the luminescence signal and the reconstituted reagent. The approximately 2-hour signal half-life provides greater flexibility in assay design. A reconstituted reagent that can be stored at room temperature for longer periods means less variability in reagent performance during long experiments or screens and less sample waste. ONE-Glo EX Reagent is the firefly luciferase detection reagent used in the Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System, allowing the same reagent to be used for single- or dual-luciferase assays.