Wizard(R) HMW DNA Extraction Kit

The Wizard HMW DNA Extraction Kit isolates high-molecular-weight DNA from white blood cells, tissue culture cells, plant tissue and Gram+ and Gram- bacteria. DNA purified with this system is particularly suitable for long-read sequencing platforms when the protocol is followed closely and DNA handled carefully. Deviating from the recommended protocol, particularly the pipette-mixing or vortexing instructions, will cause mechanical shearing of HMW DNA and decrease average fragment sizes. Such DNA shearing will negatively affect performance in long-read sequencing applications. To maximize genomic DNA size, use commonly available 1,000ul wide-bore pipette tips. Using these minimizes mechanical damage that may occur during required mixing steps. While these tips will yield larger average fragment sizes, be careful during mixing steps and when handling purified DNA after rehydration. Avoid vigorous vortexing and use 200ul wide-bore tips to homogenize rehydrated DNA samples, if necessary.