Information
This kit is recommended for users who:
- want to do 16S sequencing
- are interested in genus-level bacterial identification
Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd Party Materials" tab for more detail.
The 16S Barcoding Kit 24 V14 features:
| Feature | Property |
|---|
| Preparation time | 25 minutes + PCR |
| Input requirement | 10 ng gDNA per sample |
| PCR Required | Yes |
| Fragmentation | Not required |
| Read length | Full-length 16S gene (~1.5 kb) |
| Kit chemistry | Kit 14 |
| Associated protocols | Rapid sequencing DNA - 16S Barcoding Kit 24 V14 (SQK-16S114.24) |
| Multiplexing options | • 16S Barcoding Kit 24 V14
• Flow Cell Wash Kit |
| Pack size | 6 reactions |
| Stability | Shipped at 2–8°C
Long-term storage -20°C
Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer |
The DNA is amplified by PCR using specific 16S primers (27F and 1492R) that contain 5’ tags which facilitate the ligase-free attachment of Rapid Sequencing Adapters.
This kit has been upgraded to use our Kit 14 chemistry, which includes improved modal raw read sequencing accuracies with high output on our latest nanopore: R10.4.1. The flow cell priming and sequencing reagents are also included as part of the sequencing kit and have been reformulated to be compatible with the Kit 14 chemistry upgrade and R10.4.1 nanopore. Other updates include higher capture rate of gDNA to enable lower flow cell loading amounts, and fuel fix technology, allowing users to run longer experiments without the need for fuel addition during the run.
The kit is supported by the EPI2ME wf-16S workflow, which can be used to analyse data from the 16S Barcoding Kit 24 V14 (SQK-16S114.24) protocol. Deconvolution of barcoded sequencing data is supported by Dorado and EPI2ME which classify the barcode sequence and sort reads into corresponding folders.
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.