Information
This kit is recommended for users who:
- Want to size select to progressively deplete short fragments (<25 kb) in their sample
- Want to enrich their libraries for long fragments without using any external third-party reagents
The Short Fragment Eliminator (EXP-SFE001) is a new expansion pack that is recommended for users who want to size select high molecular weight (HMW) gDNA to deplete short fragments (<25 kb) using size selective precipitation. The kit contains Short Fragment Eliminator (SFE) buffer which progressively removes fragments up to 25 kb, with fragments under 10 kb being almost completely removed in a quick and simple workflow.
Read lengths observed when HMW gDNA was prepared using the Ligation Sequencing Kit with and without size selection (EXP-SFE001 treatment).
Note: This kit is designed to remove short DNA fragments and requires samples to be predominantly made up of HMW DNA (>48 kb). If there are large amounts of short fragments in your sample, this will result in a low yield at the end of the prep and a low sequencing output.
The Short Fragment Eliminator kit properties
| Feature | Property |
|---|
| Associated kits | All DNA sequencing kits |
| Pack size | 30 reactions |
| Kit chemsitry | Compatible with all DNA kits |
| Stability | Shipped at 2–25°C
Long-term storage 18-25°C
Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer |
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.